scholarly journals The effect of environmental factors on the differential expression of miRNAs in patients with chronic obstructive pulmonary disease: a pilot clinical study

2018 ◽  
Vol Volume 13 ◽  
pp. 741-751 ◽  
Author(s):  
Peng-Fei Liu ◽  
Peng Yan ◽  
Da-Hui Zhao ◽  
Wen-Fang Shi ◽  
Song Meng ◽  
...  
Keyword(s):  
Chronic Obstructive Pulmonary Disease ◽  
Clinical Study ◽  
Environmental Factors ◽  
Differential Expression ◽  
Pulmonary Disease ◽  
Chronic Obstructive ◽  
Obstructive Pulmonary Disease ◽  
Pilot Clinical Study

Differential expression of sputum and serum autoantibodies in patients with chronic obstructive pulmonary disease

2021 ◽  
Author(s):  
Steven P Cass ◽  
Anna Dvorkin-Gheva ◽  
Yuqiong Yang ◽  
Joshua JC McGrath ◽  
Danya Thayaparan ◽  
...  
Keyword(s):  
Chronic Obstructive Pulmonary Disease ◽  
Differential Expression ◽  
Pulmonary Disease ◽  
Differentially Expressed ◽  
Chronic Obstructive ◽  
Healthy Controls ◽  
Serum Samples ◽  
Obstructive Pulmonary Disease ◽  
Autoimmune Processes ◽  
Detectable Serum

Chronic obstructive pulmonary disease (COPD) is a complex and progressive respiratory disease. Autoimmune processes have been hypothesised to contribute to disease progression; however, the presence of autoantibodies in the serum has been variable. Given that COPD is a lung disease, we sought to investigate whether autoantibodies in sputum supernatant would better define pulmonary autoimmune processes. Matched sputum and serum samples were obtained from the Airways Disease Endotyping for Personalised Therapeutics (ADEPT) study and at the Guangzhou Institute of Respiratory Health (GIRH). Samples were collected from patients with varying severity of COPD, asymptomatic smokers and healthy control subjects. IgG and IgM autoantibodies were detected in sputum and serum of all subjects in both cohorts using a broad-spectrum autoantigen array. No differences were observed in sputum autoantibodies between COPD and asymptomatic smokers in either cohort. In contrast, 16% of detectable sputum IgG autoantibodies were decreased in COPD subjects compared to healthy controls in the ADEPT cohort. Compared to asymptomatic smokers, approximately 13% of detectable serum IgG and 40% of detectable serum IgM autoantibodies were differentially expressed in GIRH COPD subjects. Of the differentially expressed specificities, anti-nuclear autoantibodies were predominately decreased. A weak correlation between increased serum IgM anti-tissue autoantibodies and a measure of airspace enlargement was observed. The differential expression of specificities varied between the cohorts. In closing, using a comprehensive autoantibody array, we demonstrate that autoantibodies are present in COPD subjects, asymptomatic smokers and healthy controls. Cohorts displayed high levels of heterogeneity, precluding the utilisation of autoantibodies for diagnostic purposes.

scholarly journals Proteome of a Moraxella catarrhalis Strain under Iron-Restricted Conditions

2020 ◽  
Vol 9 (12) ◽  
Author(s):  
Luke V. Blakeway ◽  
Aimee Tan ◽  
Ian R. Peak ◽  
John M. Atack ◽  
Kate L. Seib
Keyword(s):  
Mass Spectrometry ◽  
Chronic Obstructive Pulmonary Disease ◽  
Differential Expression ◽  
Pulmonary Disease ◽  
Moraxella Catarrhalis ◽  
Chronic Obstructive ◽  
Iron Starvation ◽  
Data Set ◽  
Obstructive Pulmonary Disease ◽  
Content Type

Moraxella catarrhalis is a leading cause of otitis media and exacerbations of chronic obstructive pulmonary disease; however, its response to iron starvation during infection is not completely understood. Here, we announce a sequential window acquisition of all theoretical fragment ion spectra mass spectrometry (SWATH-MS) data set describing the differential expression of the M. catarrhalis CCRI-195ME proteome under iron-restricted versus iron-replete conditions.

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