scholarly journals Proton Pump Inhibitors Modulate Transport Of Doxorubicin And Its Liposomal Form Into 2D And 3D Breast Cancer Cell Cultures

2019 ◽  
Vol Volume 11 ◽  
pp. 9761-9769 ◽  
Author(s):  
Miglė Paškevičiūtė ◽  
Vilma Petrikaitė
Keyword(s):  
Breast Cancer ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cell Cultures ◽  
Proton Pump Inhibitors ◽  
Proton Pump ◽  
Liposomal Form ◽  
2D And 3D

Melatonin and vitamin D 3 increase TGF-β 1 release and induce growth inhibition in breast cancer cell cultures

2003 ◽  
Vol 110 (2) ◽  
pp. 332-337 ◽  
Author(s):  
Mariano Bizzarri ◽  
Alessandra Cucina ◽  
Maria Giovanna Valente ◽  
Fausto Tagliaferri ◽  
Valeria Borrelli ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Vitamin D ◽  
Growth Inhibition ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cell Cultures ◽  
Vitamin D 3 ◽  
Induce Growth Inhibition

Effect of CD44 Aptamer on Snail Metastasis Factor and Aggressiveness of MDA-MB-231 Breast Cancer Cell Line

2020 ◽  
Vol 21 (5) ◽  
Author(s):  
Soudabeh Kavousipour ◽  
Pooneh Mokarram ◽  
Mahdi Barazesh ◽  
Elham Arabizadeh ◽  
Vahid Razban ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Line ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Invasive Behavior ◽  
And Migration ◽  
2D And 3D ◽  
Aggressive Breast Cancer

Background: The intracellular signaling pathways stimulated by CD44/hyaluronic acid (HA) interaction play a central role in the invasion and migration of cancer cells. Epithelial-mesenchyme transition (EMT) is an important factor in cancer metastasis and migration, which can be stimulated by the snail transcription factor. Previous studies showed cells that were subjected to snail-induced EMT, characterized by a CD44high/CD24low phenotype, expressed at their surface. Objectives: The aim of this study was to assess the inhibitory effect of CD44/HA interaction on the snail expression and invasive behavior of aggressive breast cancer cell line with a high CD44 expression in 2D and 3D culture. The cell surface binding capacity of the selected aptamer was evaluated via flow cytometry assay. Methods: To test our hypothesis, we disrupted the CD44/HA interaction by DNA aptamer, which specifically binds to the Hyaluronic Acid Binding Domain (HABD) of CD44. Then, expression level of snail mRNA was evaluated in MDA-MB 231 cells, cultured in 2D and 3D conditions by real-time PCR. Furthermore, invasive behavior was evaluated, using wound healing assay. Results: The results of this study showed that CD44 aptamer reduced snail expression and invasive behavior in MDA-MB 231 cell line. In addition, our result indicated that cells cultured in 3D were more sensitive to the aptamer in comparison to those cultured in the 2D model. Conclusions: The inhibition of CD44-HA interaction, using aptamer, negatively regulates the CD44 function in aggressive breast cancer cell line with the high level of CD44 expression.

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