scholarly journals Diagnostic accuracy of droplet digital PCR for detection of EGFR T790M mutation in circulating tumor DNA

2018 ◽  
Vol Volume 10 ◽  
pp. 1209-1218 ◽  
Author(s):  
Rui Zhang ◽  
Bojiang Chen ◽  
Xiang Tong ◽  
Ye Wang ◽  
Chengdi Wang ◽  
...  
Keyword(s):  
Diagnostic Accuracy ◽  
Digital Pcr ◽  
Circulating Tumor Dna ◽  
Droplet Digital Pcr ◽  
T790m Mutation ◽  
Tumor Dna ◽  
Egfr T790m

scholarly journals The diagnostic accuracy of circulating tumor DNA for the detection of EGFR-T790M mutation in NSCLC: a systematic review and meta-analysis

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Francesco Passiglia ◽  
Sergio Rizzo ◽  
Massimo Di Maio ◽  
Antonio Galvano ◽  
Giuseppe Badalamenti ◽  
...  
Keyword(s):  
Systematic Review ◽  
Diagnostic Accuracy ◽  
Meta Analysis ◽  
Circulating Tumor Dna ◽  
T790m Mutation ◽  
Tumor Dna ◽  
Egfr T790m

scholarly journals Limited Sensitivity of Circulating Tumor DNA Detection by Droplet Digital PCR in Non-Metastatic Operable Gastric Cancer Patients

Cancers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 396 ◽  
Author(s):  
Luc Cabel ◽  
Charles Decraene ◽  
Ivan Bieche ◽  
Jean-Yves Pierga ◽  
Mostefa Bennamoun ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Gastric Adenocarcinoma ◽  
Metastatic Gastric Cancer ◽  
Digital Pcr ◽  
Circulating Tumor Dna ◽  
Allelic Frequency ◽  
Droplet Digital Pcr ◽  
Perioperative Chemotherapy ◽  
Plasma Samples ◽  
Tumor Dna

This study was designed to monitor circulating tumor DNA (ctDNA) levels during perioperative chemotherapy in patients with non-metastatic gastric adenocarcinoma. Plasma samples were prospectively collected in patients undergoing perioperative chemotherapy for non-metastatic gastric adenocarcinoma (excluding T1N0) prior to the initiation of perioperative chemotherapy, before and after surgery (NCT02220556). In each patient, mutations retrieved by targeted next-generation sequencing (NGS) on tumor samples were then tracked in circulating cell-free DNA from 4 mL of plasma by droplet digital PCR. Thirty-two patients with a diagnosis of non-metastatic gastric adenocarcinoma were included. A trackable mutation was identified in the tumor in 20 patients, seven of whom experienced relapse during follow-up. ctDNA was detectable in four patients (N = 4/19, sensitivity: 21%; 95% confidence interval CI = 8.5–43%, no baseline plasma sample was available for one patient), with a median allelic frequency (MAF) of 1.6% (range: 0.8–2.3%). No patient with available plasma samples (N = 0/18) had detectable ctDNA levels before surgery. After surgery, one of the 13 patients with available plasma samples had a detectable ctDNA level with a low allelic frequency (0.7%); this patient experienced a very short-term distant relapse only 3 months after surgery. No ctDNA was detected after surgery in the other four patients with available plasma samples who experienced a later relapse (median = 14.4, range: 9.3–26 months). ctDNA monitoring during preoperative chemotherapy and after surgery does not appear to be a useful tool in clinical practice for non-metastatic gastric cancer to predict the efficacy of chemotherapy and subsequent relapse, essentially due to the poor sensitivity of ctDNA detection.

scholarly journals Droplet digital PCR for detection and quantification of circulating tumor DNA in plasma of head and neck cancer patients

BMC Cancer ◽  
2017 ◽  
Vol 17 (1) ◽  
Author(s):  
Joost H. van Ginkel ◽  
Manon M. H. Huibers ◽  
Robert J. J. van Es ◽  
Remco de Bree ◽  
Stefan M. Willems
Keyword(s):  
Head And Neck Cancer ◽  
Head And Neck ◽  
Cancer Patients ◽  
Neck Cancer ◽  
Digital Pcr ◽  
Circulating Tumor Dna ◽  
Droplet Digital Pcr ◽  
Tumor Dna ◽  
Detection And Quantification

scholarly journals Monitoring the HER2 copy number status in circulating tumor DNA by droplet digital PCR in patients with gastric cancer

2016 ◽  
Vol 20 (1) ◽  
pp. 126-135 ◽  
Author(s):  
Katsutoshi Shoda ◽  
Daisuke Ichikawa ◽  
Yuji Fujita ◽  
Kiyoshi Masuda ◽  
Hidekazu Hiramoto ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Copy Number ◽  
Digital Pcr ◽  
Circulating Tumor Dna ◽  
Droplet Digital Pcr ◽  
Copy Number Status ◽  
Tumor Dna

Rapid clearance of circulating tumor DNA during treatment with AZD9291 of a lung cancer patient presenting the resistance EGFR T790M mutation

Lung Cancer ◽  
2016 ◽  
Vol 91 ◽  
pp. 73-74 ◽  
Author(s):  
Audrey Vallée ◽  
Clarisse Audigier-Valette ◽  
Guillaume Herbreteau ◽  
Julien Merrien ◽  
Laurent Tessonnier ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Patient ◽  
Lung Cancer Patient ◽  
Circulating Tumor Dna ◽  
T790m Mutation ◽  
Rapid Clearance ◽  
Tumor Dna ◽  
Egfr T790m

Abstract 2408: A novel multiplex droplet digital PCR approach to KRAS mutation detection in circulating tumor DNA

2015 ◽  
Author(s):  
Alexandra Pender ◽  
Isaac Garcia-Murillas ◽  
Sareena Rana ◽  
David Gonzalez de Castro ◽  
Nicholas Turner ◽  
...  
Keyword(s):  
Kras Mutation ◽  
Mutation Detection ◽  
Digital Pcr ◽  
Circulating Tumor Dna ◽  
Droplet Digital Pcr ◽  
Tumor Dna
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