scholarly journals Accelerated Barrier Repair in Human Skin Explants Induced with a Plant-Derived PPAR-α Activating Complex via Cooperative Interactions

2021 ◽  
Vol Volume 14 ◽  
pp. 1271-1293
Author(s):  
George Majewski ◽  
John Craw ◽  
Timothy Falla
Keyword(s):  
Human Skin ◽  
Cooperative Interactions ◽  
Skin Explants ◽  
Barrier Repair

scholarly journals HIV-1 Selection by Epidermal Dendritic Cells during Transmission across Human Skin

1998 ◽  
Vol 187 (10) ◽  
pp. 1623-1631 ◽  
Author(s):  
Jeanette C. Reece ◽  
Amanda J. Handley ◽  
E. John Anstee ◽  
Wayne A. Morrison ◽  
Suzanne M. Crowe ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Human Skin ◽  
Cell Sorting ◽  
Virus Entry ◽  
Skin Explants ◽  
Skin Uptake ◽  
Virus Exposure ◽  
Epidermal Dendritic Cells ◽  
Hiv 1

Macrophage tropic HIV-1 is predominant during the initial viremia after person to person transmission of HIV-1 (Zhu, T., H. Mo, N. Wang, D.S. Nam, Y. Cao, R.A. Koup, and D.D. Ho. 1993. Science. 261:1179–1181.), and this selection may occur during virus entry and carriage to the lymphoid tissue. Human skin explants were used to model HIV-1 selection that may occur at the skin or mucosal surface. Macrophage tropic, but not T cell line tropic strains of HIV-1 applied to the abraded epidermis were recovered from the cells emigrating from the skin explants. Dermis and epidermis were separated by dispase digestion after virus exposure to determine the site of viral selection within the skin. Uptake and transmission to T cells of all HIV-1 isolates was found with the dermal emigrant cells, but only macrophage tropic virus was transferred by emigrants from the epidermis exposed to HIV-1, indicating selection only within the epidermis. CD3+, CD4+ T cells were found in both the dermal and epidermal emigrant cells. After cell sorting to exclude contaminating T cells, macrophage tropic HIV-1 was found in both the dermal emigrant dendritic cells and in dendritic cells sorted from the epidermal emigrants. These observations suggest that selective infection of the immature epidermal dendritic cells represents the cellular mechanism that limits the initial viremia to HIV-1 that can use the CCR5 coreceptor.

scholarly journals Effect of complexing lipids on cellular uptake and expression of messenger RNA in human skin explants

2020 ◽  
Author(s):  
Anna K. Blakney ◽  
Polina Deletic ◽  
Paul F. McKay ◽  
Clément R. Bouton ◽  
Marianne Ashford ◽  
...  
Keyword(s):  
Human Skin ◽  
Cellular Uptake ◽  
Messenger Rna ◽  
Skin Explants

Assessment of the biochemical effects of percutaneous exposure of sulphur mustard in an in vitro human skin system

1996 ◽  
Vol 15 (3) ◽  
pp. 237-244 ◽  
Author(s):  
CD Lindsay ◽  
P. Rice
Keyword(s):  
Inflammatory Response ◽  
Human Skin ◽  
Serine Proteases ◽  
Inflammatory Cells ◽  
Chemical Warfare Agent ◽  
Skin Damage ◽  
Sulphur Mustard ◽  
Nuclear Pyknosis ◽  
Skin Explants

1 Sulphur mustard (HD) is a potent chemical warfare agent which causes incapacitating blisters on human skin. There is no specific pretreatment nor therapy against this agent and the mechanism of dermo-epidermal cleavage is unclear. The aim of this study was to use a human skin explant system to determine the consequences of percuta neous exposure to HD. 2 Increased activities of serine proteases associated with blistering disorders in humans were detected from human skin explants after exposure to HD. The most consistent response and the highest protease activities measured were found for trypsin. This class of enzyme is therefore implicated in the dermo-epidermal separation which is associated with blistering in humans following exposure to HD. 3 An inflammatory response was observed in the skin explants exposed to HD. At low doses of HD it was characterised by the presence of neutrophils in the papillary dermis, culminating in the infiltration of the epidermis by these inflammatory cells at higher concen trations of HD. A variety of other histopathological changes in the explants was found such as focal dermo- epidermal separation, nuclear pyknosis and perinuclear vacuolation. 4 The study indicates that full thickness human skin explants can be used to investigate various aspects of the possible pathogenesis of HD-induced skin damage, in cluding the associated inflammatory response.

Cutaneous DNA delivery and gene expression in ex vivo human skin explants via wet-etch microfabricated microneedles

2005 ◽  
Vol 13 (7) ◽  
pp. 415-421 ◽  
Author(s):  
James Birchall ◽  
Sion Coulman ◽  
Marc Pearton ◽  
Chris Allender ◽  
Keith Brain ◽  
...  
Keyword(s):  
Gene Expression ◽  
Human Skin ◽  
Ex Vivo ◽  
Dna Delivery ◽  
Skin Explants ◽  
Wet Etch

scholarly journals Effect of human skin explants on the neurite growth of the PC12 cell line

2013 ◽  
Vol 22 (3) ◽  
pp. 224-225 ◽  
Author(s):  
Nicolas Lebonvallet ◽  
Jean-Pierre Pennec ◽  
Christelle Le Gall ◽  
Ulysse Pereira ◽  
Nicholas Boulais ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Skin ◽  
Pc12 Cell ◽  
Neurite Growth ◽  
Pc12 Cell Line ◽  
Skin Explants

scholarly journals Compromising human skin in vivo and ex vivo to study skin barrier repair

2019 ◽  
Vol 1864 (8) ◽  
pp. 1103-1108 ◽  
Author(s):  
T. Berkers ◽  
W.A. Boiten ◽  
S. Absalah ◽  
J. van Smeden ◽  
A.P.M. Lavrijsen ◽  
...  
Keyword(s):  
Human Skin ◽  
Ex Vivo ◽  
Skin Barrier ◽  
Barrier Repair ◽  
Skin Barrier Repair
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