scholarly journals Calcium hydroxylapatite treatment of human skin: evidence of collagen turnover through picrosirius red staining and circularly polarized microscopy

2018 ◽  
Vol Volume 11 ◽  
pp. 29-35 ◽  
Author(s):  
Nicola Zerbinati ◽  
Alberto Calligaro
Keyword(s):  
Human Skin ◽  
Collagen Turnover ◽  
Circularly Polarized ◽  
Calcium Hydroxylapatite ◽  
Polarized Microscopy ◽  
Picrosirius Red

scholarly journals Microscopic and ultrastructural evidences in human skin following calcium hydroxylapatite filler treatment

2017 ◽  
Vol 309 (5) ◽  
pp. 389-396 ◽  
Author(s):  
Nicola Zerbinati ◽  
Edoardo D’Este ◽  
Pier Camillo Parodi ◽  
Alberto Calligaro
Keyword(s):  
Human Skin ◽  
Calcium Hydroxylapatite

Optimization of Picrosirius red staining protocol to determine collagen fiber orientations in vaginal and uterine cervical tissues by Mueller polarized microscopy

2015 ◽  
Vol 78 (8) ◽  
pp. 723-730 ◽  
Author(s):  
André Nazac ◽  
Stéphane Bancelin ◽  
Benjamin Teig ◽  
Bicher Haj Ibrahim ◽  
Hervé Fernandez ◽  
...  
Keyword(s):  
Collagen Fiber ◽  
Polarized Microscopy ◽  
Staining Protocol ◽  
Picrosirius Red

Layer-Specific Distributed Collagen Fiber Orientations in Human Arteries, From Thoracic Aorta to Common Iliac

2011 ◽  
Author(s):  
Andreas J. Schriefl ◽  
Peter Regitnig ◽  
David M. Pierce ◽  
Gerhard A. Holzapfel
Keyword(s):  
Collagen Fiber ◽  
Aortic Wall ◽  
Dispersion Model ◽  
Tissue Samples ◽  
Iliac Arteries ◽  
Polarized Microscopy ◽  
Dispersion Data ◽  
Human Arteries ◽  
Thoracic And Abdominal ◽  
Picrosirius Red

We use the well established method of polarized microscopy on picrosirius red stained tissue samples and a dispersion model to quantify over 37000 measured layer-specific 3D fiber orientations of 11 human thoracic and abdominal aortas and common iliac arteries. Our results yield mean fiber directions, quantitative dispersion data and the existence of two distinct and prominent fiber families for all layers of the aortic wall.

scholarly journals Targeting Collagen Type III in Proteinuric Kidney Disease: Informing Drug Potential Using the Jaccard–Tanimoto Index

Processes ◽  
2020 ◽  
Vol 8 (8) ◽  
pp. 996
Author(s):  
Michelle Liu ◽  
Anoushka Dalvi ◽  
Sony Dalapati ◽  
Natalia Prakash ◽  
Zhijian Hu ◽  
...  
Keyword(s):  
Kidney Disease ◽  
Collagen Type ◽  
Mrna Level ◽  
Immunohistochemical Analysis ◽  
Fold Increase ◽  
Collagen Type Iii ◽  
Type Iii ◽  
Polarized Microscopy ◽  
Picrosirius Red ◽  
Bowman's Capsule

Collagenofibrotic glomerulopathy, a collagen type III kidney disease, is associated with proteinuria and accumulation ofcollagen type III in the glomerulus specifically the mesangium and/or capillary walls. The puromcyin aminonucleoside (PAN) nephropathy model was evaluated to examine the relation between COL3A1 mRNA and proteinuria. In Wistar rats administered PAN, a robust increase in proteinuria was accompanied by glomerular hypertrophy and expansion of both the Bowman’s capsule and Bowman’s space. An ~4-fold increase in renal COL3A1 mRNA was observed in the PAN cohort with urine protein exhibiting a direct (r = 0.8) and significant correlation with kidney COL3A1 mRNA level. Both Picrosirius red polarized microscopy and immunohistochemical analysis showed localization of collagen type III to the glomerular mesangium. Gene ontology-driven transcriptomic analysis reveals a robust COL3A1 network in the glomerular compartment.

scholarly journals Regulation of Collagen Turnover in Human Skin Fibroblasts Exposed to a Gadolinium-Based Contrast Agent

2009 ◽  
Vol 44 (8) ◽  
pp. 433-439 ◽  
Author(s):  
Narasimharao Bhagavathula ◽  
Marissa DaSilva ◽  
Muhammad N. Aslam ◽  
Michael K. Dame ◽  
Roscoe L. Warner ◽  
...  
Keyword(s):  
Contrast Agent ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Collagen Turnover ◽  
Human Skin Fibroblasts

A web-like network of type vi collagen in human skin is revealed by immuno-electron microscopy

1988 ◽  
Vol 46 ◽  
pp. 382-383
Author(s):  
Douglas R. Keene ◽  
Robert W. Glanville ◽  
Eva Engvall
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Human Skin ◽  
Basement Membranes ◽  
Primary Antibody ◽  
Fat Cells ◽  
Secondary Antibody ◽  
Type Vi Collagen ◽  
Dermal Matrix ◽  
Immuno Electron Microscopy

A mouse monoclonal antibody (5C6) prepared against human type VI collagen (1) has been used in this study to immunolocalize type VI collagen in human skin. The enbloc method used involves exposing whole tissue pieces to primary antibody and 5 nm gold conjugated secondary antibody before fixation, and has been described in detail elsewhere (2).Biopsies were taken from individuals ranging in age from neonate to 65 years old. By immuno-electron microscopy, type VI collagen is found to be distributed as a fine branching network closely associated with (but not attached to) banded collagen fibrils containing types I and III collagen (Fig. 1). It appears to enwrap fibers, to weave between individual fibrils within a fiber, and to span the distance separating fibers, creating a “web-like network” which entraps fibers within deep papillary and reticular dermal layers (Fig. 2). Relative to that in the dermal matrix, the concentration of type VI collagen is higher around endothelial basement membranes limiting the outer boundaries of nerves, capillaries, and fat cells (Fig. 3).

Effect of Acetone and Kerosene on Skin Ultrastructure

1972 ◽  
Vol 30 ◽  
pp. 92-93
Author(s):  
A. P. Lupulescu ◽  
H. Pinkus ◽  
D. J. Birmingham
Keyword(s):  
Electron Microscopy ◽  
Human Skin ◽  
Osmium Tetroxide ◽  
Human Epidermis ◽  
Ultrastructural Changes ◽  
Chemical Agents ◽  
Skin Ultrastructure ◽  
Volar Surface

Our laboratory is engaged in the study of the effect of different chemical agents on human skin, using electron microscopy. Previous investigations revealed that topical use of a strong alkali (NaOH 1N) or acid (HCl 1N), induces ultrastructural changes in the upper layers of human epidermis. In the current experiments, acetone and kerosene, which are primarily lipid solvents, were topically used on the volar surface of the forearm of Caucasian and Negro volunteers. Skin specimens were bioptically removed after 90 min. exposure and 72. hours later, fixed in 3% buffered glutaraldehyde, postfixed in 1% phosphate osmium tetroxide, then flat embedded in Epon.

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