scholarly journals Stem cell-mediated osteogenesis: therapeutic potential for bone tissue engineering

2012 ◽  
pp. 47 ◽  
Author(s):  
Josh Neman ◽  
Amanda Hambrecht ◽  
Rahul Jandial ◽  
Cadry
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Therapeutic Potential

scholarly journals Pretreating mesenchymal stem cells with electrical stimulation causes sustained long-lasting pro-osteogenic effects

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4959 ◽  
Author(s):  
Maria Eischen-Loges ◽  
Karla M.C. Oliveira ◽  
Mit B. Bhavsar ◽  
John H. Barker ◽  
Liudmila Leppik
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Electrical Stimulation ◽  
Bone Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Therapeutic Potential ◽  
Osteogenic Marker ◽  
Osteogenic Activity ◽  
Osteogenic Effect

Background Electrical stimulation (ES) has a long history of successful use in the clinical treatment of refractory, non-healing bone fractures and has recently been proposed as an adjunct to bone tissue-engineering treatments to optimize their therapeutic potential. This idea emerged from ES’s demonstrated positive effects on stem cell migration, proliferation, differentiation and adherence to scaffolds, all cell behaviors recognized to be advantageous in Bone Tissue Engineering (BTE). In previous in vitro experiments we demonstrated that direct current ES, administered daily, accelerates Mesenchymal Stem Cell (MSC) osteogenic differentiation. In the present study, we sought to define the optimal ES regimen for maximizing this pro-osteogenic effect. Methods Rat bone marrow-derived MSC were exposed to 100 mV/mm, 1 hr/day for three, seven, and 14 days, then osteogenic differentiation was assessed at Day 14 of culture by measuring collagen production, calcium deposition, alkaline phosphatase activity and osteogenic marker gene expression. Results We found that exposing MSC to ES for three days had minimal effect, while seven and 14 days resulted in increased osteogenic differentiation, as indicated by significant increases in collagen and calcium deposits, and expression of osteogenic marker genes Col1a1, Osteopontin, Osterix and Calmodulin. We also found that cells treated with ES for seven days, maintained this pro-osteogenic activity long (for at least seven days) after discontinuing ES exposure. Discussion This study showed that while three days of ES is insufficient to solicit pro-osteogenic effects, seven and 14 days significantly increases osteogenic differentiation. Importantly, we found that cells treated with ES for only seven days, maintained this pro-osteogenic activity long after discontinuing ES exposure. This sustained positive osteogenic effect is likely due to the enhanced expression of RunX2 and Calmodulin we observed. This prolonged positive osteogenic effect, long after discontinuing ES treatment, if incorporated into BTE treatment protocols, could potentially improve outcomes and in doing so help BTE achieve its full therapeutic potential.

Osteon-mimetic 3D nanofibrous scaffold enhancing stem cell proliferation and osteogenic differentiation for bone regeneration

2022 ◽  
Author(s):  
Ting Song ◽  
Jianhua Zhou ◽  
Ming Shi ◽  
Liuyang Xuan ◽  
Huamin Jiang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Nanofibrous Scaffold ◽  
Hierarchical Microstructure ◽  
Stem Cell Proliferation

Scaffold microstructure is important for bone tissue engineering. Failure to synergistically imitate the hierarchical microstructure of bone component, such as osteon with concentric multilayers assembled by nanofibers, hindered the performance...

scholarly journals Stem Cell Based Bone Tissue Engineering

10.5772/33102 ◽  
2012 ◽  
Author(s):  
Lauren Vernon ◽  
Lee Kaplan ◽  
Chun-Yuh Charles
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Tissue

In Situ Bone Tissue Engineering With an Endogenous Stem Cell Mobilizer and Osteoinductive Nanofibrous Polymeric Scaffolds

2017 ◽  
Vol 12 (12) ◽  
pp. 1700062 ◽  
Author(s):  
Jong Seung Lee ◽  
Yoonhee Jin ◽  
Hyun-Ji Park ◽  
Kisuk Yang ◽  
Min Suk Lee ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Polymeric Scaffolds ◽  
Endogenous Stem Cell

scholarly journals Dual non-viral gene delivery from microparticles within 3D high-density stem cell constructs for enhanced bone tissue engineering

Biomaterials ◽  
2018 ◽  
Vol 161 ◽  
pp. 240-255 ◽  
Author(s):  
Alexandra McMillan ◽  
Minh Khanh Nguyen ◽  
Tomas Gonzalez-Fernandez ◽  
Peilin Ge ◽  
Xiaohua Yu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Gene Delivery ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
High Density ◽  
Viral Gene ◽  
Viral Gene Delivery

scholarly journals Characterization and osteogenic evaluation of mesoporous magnesium–calcium silicate/polycaprolactone/polybutylene succinate composite scaffolds fabricated by rapid prototyping

RSC Advances ◽  
2018 ◽  
Vol 8 (59) ◽  
pp. 33882-33892 ◽  
Author(s):  
Yun Gyeong Kang ◽  
Jie Wei ◽  
Ji Eun Kim ◽  
Yan Ru Wu ◽  
Eun Jin Lee ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Rapid Prototyping ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Calcium Silicate ◽  
Composite Scaffold ◽  
Composite Scaffolds ◽  
Polybutylene Succinate

A new composite scaffold consisting of mesoporous magnesium–calcium silicate (m_MCS), polycaprolactone (PCL), and polybutylene succinate (PBSu) was manufactured by a rapid prototyping technique, for stem cell-based bone tissue engineering.

Bone tissue engineering with scaffold-supported perfusion co-cultures of human stem cell-derived osteoblasts and cell line-derived osteoclasts

2017 ◽  
Vol 59 ◽  
pp. 303-311 ◽  
Author(s):  
Işıl G. Beşkardeş ◽  
Rebecca S. Hayden ◽  
Dean L. Glettig ◽  
David L. Kaplan ◽  
Menemşe Gümüşderelioğlu
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Bone Tissue Engineering ◽  
Cell Line ◽  
Bone Tissue ◽  
Human Stem Cell

Core–shell fibrous stem cell carriers incorporating osteogenic nanoparticulate cues for bone tissue engineering

2015 ◽  
Vol 28 ◽  
pp. 183-192 ◽  
Author(s):  
Jennifer Olmos Buitrago ◽  
Roman A. Perez ◽  
Ahmed El-Fiqi ◽  
Rajendra K. Singh ◽  
Joong-Hyun Kim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cell ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Core Shell ◽  
Cell Carriers
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