scholarly journals Highly Efficient Oligonucleotide Transfer into Intact Yeast Cells Using Square-Wave Pulse Electroporation

BioTechniques ◽  
1998 ◽  
Vol 25 (2) ◽  
pp. 294-296 ◽  
Author(s):  
François-Xavier Barre ◽  
Lluis M. Mir ◽  
Yann Lécluse ◽  
Annick Harel-Bellan
Keyword(s):  
Yeast Cells ◽  
Wave Pulse ◽  
Square Wave ◽  
Highly Efficient

scholarly journals Compact Square-Wave Pulse Electroporator with Controlled Electroporation Efficiency and Cell Viability

Symmetry ◽  
2020 ◽  
Vol 12 (3) ◽  
pp. 412 ◽  
Author(s):  
Voitech Stankevic ◽  
Povilas Simonis ◽  
Nerija Zurauskiene ◽  
Arunas Stirke ◽  
Aldas Dervinis ◽  
...  
Keyword(s):  
Cell Viability ◽  
Current Pulse ◽  
Pulse Generator ◽  
Yeast Cells ◽  
Load Impedance ◽  
Wave Pulse ◽  
Square Wave ◽  
Unit Design ◽  
Pulse Form ◽  
Electroporation Efficiency

The design and development of a compact square-wave pulse generator for the electroporation of biological cells is presented. This electroporator can generate square-wave pulses with durations from 3 μs up to 10 ms, voltage amplitudes up to 3500 V, and currents up to 250 A. The quantity of the accumulated energy is optimized by means of a variable capacitor bank. The pulse forming unit design uses a crowbar circuit, which gives better control of the pulse form and its duration, independent of the load impedance. In such cases, the square-wave pulse form ensures better control of electroporation efficiency by choosing parameters determined in advance. The device has an integrated graphic LCD screen and measurement modules for the visualization of the current pulse, allowing for express control of the electroporation quality and does not require an external oscilloscope for current pulse recording. This electroporator was tested on suspensions of Saccharomyces cerevisiae yeast cells, during which, it was demonstrated that the application of such square-wave pulses ensured better control of the electroporation efficiency and cell viability after treatment using the pulsed electric field (PEF).

Influence of nonuniform charging on flat of output square-wave pulse of pulse forming system

2013 ◽  
Vol 25 (5) ◽  
pp. 1320-1324
Author(s):  
樊旭亮 Fan Xuliang ◽  
刘金亮 Liu Jinliang ◽  
张瑜 Zhang Yu
Keyword(s):  
Wave Pulse ◽  
Square Wave

Incremental and decremental L- and M-cone-driven ERG responses: I Square-wave pulse stimulation

2014 ◽  
Vol 31 (4) ◽  
pp. A159 ◽  
Author(s):  
Declan McKeefry ◽  
Jan Kremers ◽  
Deepika Kommanapalli ◽  
Naveen K. Challa ◽  
Ian J. Murray ◽  
...  
Keyword(s):  
Wave Pulse ◽  
Square Wave ◽  
Pulse Stimulation

Application of square wave pulse-width-variable laser using temporal shaping

2004 ◽  
Author(s):  
Li Wang ◽  
Yu Zhao ◽  
Yong Tian ◽  
Jinfeng Zhou ◽  
Liu Huang
Keyword(s):  
Pulse Width ◽  
Wave Pulse ◽  
Square Wave

Dual square-wave pulse passively mode-locked fiber laser

2017 ◽  
Vol 56 (17) ◽  
pp. 4934 ◽  
Author(s):  
Qingchao Su ◽  
Tianshu Wang ◽  
Jing Zhang ◽  
Wanzhuo Ma ◽  
Peng Liu ◽  
...  
Keyword(s):  
Fiber Laser ◽  
Wave Pulse ◽  
Square Wave

Duty Ratio Thresholds for Regular Transmission at Human Tissue of Near-Infrared Square-Wave Pulse

2013 ◽  
Vol 333-335 ◽  
pp. 555-558
Author(s):  
Jian Wang ◽  
Shuo Guo Li
Keyword(s):  
Experimental Study ◽  
Human Tissue ◽  
Near Infrared ◽  
Transmission Mechanism ◽  
Duty Ratio ◽  
Human Hand ◽  
Light Emitting ◽  
Wave Pulse ◽  
Square Wave ◽  
Important Index

In order to understand the transmission mechanism at human tissue of near-infrared square-wave pulse (NIRSP), an experimental study of the duty ratio threshold for regular transmission at the fingertip and palm of human hand by NIRSP was carried out by using a couple of simple transmitting and receiving device with tunable duty ratios, and also the light emitting diodes (LED) both at the wavelengths of 850 nm and 940 nm. The thresholds were determined by measuring the waveforms of the changed voltages of NIRSP receiving system as a measurement of the transmission at human tissue. When the receiving waveforms were taken on the certain behavior as the emitting waveforms, the duty ratio threshold for regular transmission at human tissue of NIRSP was observed. The experimental results show that the duty ratio is an important index for the transmission at human tissue of NIRSP. And the duty ratio thresholds for regular transmission both at the fingertip and palm of NIRSP at the wavelength of 850 nm and 940 nm have been given.

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