scholarly journals DNA Sequencing by Capillary Electrophoresis Using Short Oligonucleotide Primer Libraries

BioTechniques ◽  
1996 ◽  
Vol 20 (6) ◽  
pp. 1058-1069 ◽  
Author(s):  
M.C. Ruiz-Martinez ◽  
E. Carrilho ◽  
J. Berka ◽  
J. Kieleczawa ◽  
A.W. Miller ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Dna Sequencing ◽  
Oligonucleotide Primer ◽  
Short Oligonucleotide

Star-shaped polymers for DNA sequencing by capillary electrophoresis

2011 ◽  
Vol 1218 (20) ◽  
pp. 3037-3041 ◽  
Author(s):  
Fan Gao ◽  
Cai Tie ◽  
Xin-Xiang Zhang ◽  
Zhiqiang Niu ◽  
Xiaojin He ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Dna Sequencing

Evaluation of the Potential of a Charge-Injection Device for DNA Sequencing by Multiplexed Capillary Electrophoresis

1995 ◽  
Vol 49 (6) ◽  
pp. 825-833 ◽  
Author(s):  
Qingbo Li ◽  
Edward S. Yeung
Keyword(s):  
Capillary Electrophoresis ◽  
Dna Sequencing ◽  
Fluorescence Detection ◽  
Charge Injection ◽  
Laser Induced Fluorescence ◽  
Array Detector ◽  
Injection Device ◽  
Laser Induced Fluorescence Detection ◽  
Array Detectors ◽  
Multiplexed Capillary Electrophoresis

Despite the rapid growth in the use of imaging detectors in spectroscopy, the charge-injection device (CID) has unique features that have not been fully exploited. The advantages of the CID as a two-dimensional array detector for laser-induced fluorescence detection in highly multiplexed capillary electrophoresis are evaluated. In such a system, the CID maintains both high sensitivity and high sampling rate, which are usually difficult to achieve simultaneously with other array detectors. Applying the electronic windowing function significantly improves the scan rate and greatly reduces the volume of data generated. With 1-s exposure time and 488-nm excitation, the detection limit of the system is 10−12 M fluorescein with the device cryogenically cooled and 10−11 M fluorescein at ambient temperature. The low dark current of the CID imager allows operation at room temperature without significantly affecting sensitivity when combined with moderate laser powers. We demonstrate that the CID is well suited for high-speed, high-throughput DNA sequencing based on multiplexed capillary electrophoresis with on-column laser-induced fluorescence detection.

High-speed separations of DNA sequencing reactions by capillary electrophoresis

1990 ◽  
Vol 62 (9) ◽  
pp. 900-903 ◽  
Author(s):  
Howard. Drossman ◽  
John A. Luckey ◽  
Anthony J. Kostichka ◽  
Jonathan. D'Cunha ◽  
Lloyd M. Smith
Keyword(s):  
Capillary Electrophoresis ◽  
Dna Sequencing ◽  
High Speed
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