scholarly journals Automated one-step DNA sequencing based on nanoliter reaction volumes and capillary electrophoresis

2000 ◽  
Vol 28 (15) ◽  
pp. 73e-73 ◽  
Author(s):  
H.-m. Pang
Keyword(s):  
Capillary Electrophoresis ◽  
Dna Sequencing ◽  
One Step ◽  
Reaction Volumes

scholarly journals DNA Sequencing by Capillary Electrophoresis Using Short Oligonucleotide Primer Libraries

BioTechniques ◽  
1996 ◽  
Vol 20 (6) ◽  
pp. 1058-1069 ◽  
Author(s):  
M.C. Ruiz-Martinez ◽  
E. Carrilho ◽  
J. Berka ◽  
J. Kieleczawa ◽  
A.W. Miller ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Dna Sequencing ◽  
Oligonucleotide Primer ◽  
Short Oligonucleotide

scholarly journals A Method For Parallel, Automated, Thermal Cycling of Submicroliter Samples

2001 ◽  
Vol 11 (3) ◽  
pp. 441-447
Author(s):  
Jonathan Nakane ◽  
David Broemeling ◽  
Roger Donaldson ◽  
Andre Marziali ◽  
Thomas D. Willis ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Thermal Cycling ◽  
High Throughput ◽  
Dna Analysis ◽  
Detection Efficiency ◽  
Large Fraction ◽  
Cycle Sequencing ◽  
High Throughput Dna Sequencing ◽  
Reaction Volumes ◽  
The Cost

A large fraction of the cost of DNA sequencing and other DNA-analysis processes results from the reagent costs incurred during cycle sequencing or PCR. In particular, the high cost of the enzymes and dyes used in these processes often results in thermal cycling costs exceeding $0.50 per sample. In the case of high-throughput DNA sequencing, this is a significant and unnecessary expense. Improved detection efficiency of new sequencing instrumentation allows the reaction volumes for cycle sequencing to be scaled down to one-tenth of presently used volumes, resulting in at least a 10-fold decrease in the cost of this process. However, commercially available thermal cyclers and automated reaction setup devices have inherent design limitations which make handling volumes of <1 μL extremely difficult. In this paper, we describe a method for thermal cycling aimed at reliable, automated cycling of submicroliter reaction volumes.

Star-shaped polymers for DNA sequencing by capillary electrophoresis

2011 ◽  
Vol 1218 (20) ◽  
pp. 3037-3041 ◽  
Author(s):  
Fan Gao ◽  
Cai Tie ◽  
Xin-Xiang Zhang ◽  
Zhiqiang Niu ◽  
Xiaojin He ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Dna Sequencing

Evaluation of the Potential of a Charge-Injection Device for DNA Sequencing by Multiplexed Capillary Electrophoresis

1995 ◽  
Vol 49 (6) ◽  
pp. 825-833 ◽  
Author(s):  
Qingbo Li ◽  
Edward S. Yeung
Keyword(s):  
Capillary Electrophoresis ◽  
Dna Sequencing ◽  
Fluorescence Detection ◽  
Charge Injection ◽  
Laser Induced Fluorescence ◽  
Array Detector ◽  
Injection Device ◽  
Laser Induced Fluorescence Detection ◽  
Array Detectors ◽  
Multiplexed Capillary Electrophoresis

Despite the rapid growth in the use of imaging detectors in spectroscopy, the charge-injection device (CID) has unique features that have not been fully exploited. The advantages of the CID as a two-dimensional array detector for laser-induced fluorescence detection in highly multiplexed capillary electrophoresis are evaluated. In such a system, the CID maintains both high sensitivity and high sampling rate, which are usually difficult to achieve simultaneously with other array detectors. Applying the electronic windowing function significantly improves the scan rate and greatly reduces the volume of data generated. With 1-s exposure time and 488-nm excitation, the detection limit of the system is 10−12 M fluorescein with the device cryogenically cooled and 10−11 M fluorescein at ambient temperature. The low dark current of the CID imager allows operation at room temperature without significantly affecting sensitivity when combined with moderate laser powers. We demonstrate that the CID is well suited for high-speed, high-throughput DNA sequencing based on multiplexed capillary electrophoresis with on-column laser-induced fluorescence detection.

High-speed separations of DNA sequencing reactions by capillary electrophoresis

1990 ◽  
Vol 62 (9) ◽  
pp. 900-903 ◽  
Author(s):  
Howard. Drossman ◽  
John A. Luckey ◽  
Anthony J. Kostichka ◽  
Jonathan. D'Cunha ◽  
Lloyd M. Smith
Keyword(s):  
Capillary Electrophoresis ◽  
Dna Sequencing ◽  
High Speed
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