scholarly journals Efficient micro-recovery and guanidination of peptides directly from MALDI target spots

BioTechniques ◽  
2005 ◽  
Vol 38 (2) ◽  
pp. 301-304 ◽  
Author(s):  
Steven L. Cockrill ◽  
Kelly L. Foster ◽  
Justin Wildsmith ◽  
April R. Goodrich ◽  
John G. Dapron ◽  
...  
Keyword(s):  
Maldi Target

On-MALDI-Target N-Glycan Nonreductive Amination by 2-Aminobenzoic Acid

2020 ◽  
Vol 92 (15) ◽  
pp. 10252-10256
Author(s):  
Xiaoping L Hronowski ◽  
Yan Wang ◽  
Zoran Sosic ◽  
Ru Wei
Keyword(s):  
Aminobenzoic Acid ◽  
Maldi Target

TiO2-modified MALDI target for in vitro modeling of the oxidative biotransformation of diclofenac

2020 ◽  
Vol 30 (2) ◽  
pp. 220-222
Author(s):  
Alexander Yu. Gorbunov ◽  
Konstantin A. Krasnov ◽  
Alexander A. Bardin ◽  
Olga A. Keltsieva ◽  
Vladimir N. Babakov ◽  
...  
Keyword(s):  
Maldi Target ◽  
In Vitro Modeling

Development of silicon microstructures and thin-film MALDI target plates for automated proteomics sample identifications

2001 ◽  
Vol 109 (1) ◽  
pp. 41-46 ◽  
Author(s):  
Tasso Miliotis ◽  
György Marko-Varga ◽  
Johan Nilsson ◽  
Thomas Laurell
Keyword(s):  
Thin Film ◽  
Maldi Target ◽  
Silicon Microstructures

Sample Preparation for MALDI Mass Spectrometry Using an Elastomeric Device Reversibly Sealed on the MALDI Target

2006 ◽  
Vol 78 (17) ◽  
pp. 6160-6168 ◽  
Author(s):  
Xiaoxi Chen ◽  
Anthony Murawski ◽  
Guannan Kuang ◽  
Daniel J. Sexton ◽  
William Galbraith
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Maldi Mass Spectrometry ◽  
Maldi Target

scholarly journals MALDI-target integrated platform for affinity-captured protein digestion

2014 ◽  
Vol 807 ◽  
pp. 1-8 ◽  
Author(s):  
Asilah Ahmad-Tajudin ◽  
Belinda Adler ◽  
Simon Ekström ◽  
György Marko-Varga ◽  
Johan Malm ◽  
...  
Keyword(s):  
Protein Digestion ◽  
Maldi Target ◽  
Integrated Platform

On-target trypsinization on MALDI target for MS

2005 ◽  
Vol 40 (11) ◽  
pp. 1503-1504 ◽  
Author(s):  
H. Kajiwara
Keyword(s):  
Maldi Target

MALDI-TOF mass spectrometry for antimicrobial susceptibility testing

2021 ◽  
Author(s):  
Evgeny A. Idelevich ◽  
Karsten Becker
Keyword(s):  
Mass Spectrometry ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Isotope Labeling ◽  
Antimicrobial Susceptibility Testing ◽  
Maldi Tof Ms ◽  
Maldi Target ◽  
Maldi Tof ◽  
Antibiotic Susceptibility Test ◽  
Tof Ms

The advent of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) in clinical microbiology has dramatically improved the accuracy and speed of diagnostics. However, this progress has mainly been limited to the identification of microorganisms, whereas the practical improvement of antimicrobial susceptibility testing (AST) still lags behind. MALDI-TOF MS-based approaches include the detection of selected resistance mechanisms and the universal phenotypic AST. This minireview focuses on the discussion of those MALDI-TOF MS methods that allow universal growth-based phenotypic AST. The method of minimal profile change concentrations (MPCC) is based on detecting proteome modification in presence of an antimicrobial. Using stable-isotope labeling, characteristic mass shifts in the presence of an antimicrobial indicate the incorporation of the isotopic labels, and, thus, the viability and resistance of the microorganism. For MALDI Biotyper antibiotic susceptibility test rapid assay (MBT-ASTRA), microorganisms are incubated with or without an antimicrobial, followed by cell lysis, protein extraction, and transfer of the cell lysate onto a MALDI target plate. Using the internal standard, peak intensities are correlated to the amount of microbial proteins, and the relative microbial growth is calculated. Most recent development in the field is the direct-on-target microdroplet growth assay (DOT-MGA). Here, incubation of microorganisms with antimicrobials takes place directly on spots of a MALDI target in form of microdroplets. After incubation, nutrient medium is removed by dabbing with absorptive material. Resistant microorganisms grow despite the presence of antimicrobial, and their amplified biomass is detected by MALDI-TOF MS. Finally, an outlook is provided for further assay improvements.

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