Sample Preparation for MALDI Mass Spectrometry Using an Elastomeric Device Reversibly Sealed on the MALDI Target

2006 ◽  
Vol 78 (17) ◽  
pp. 6160-6168 ◽  
Author(s):  
Xiaoxi Chen ◽  
Anthony Murawski ◽  
Guannan Kuang ◽  
Daniel J. Sexton ◽  
William Galbraith
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Maldi Mass Spectrometry ◽  
Maldi Target

scholarly journals Sample preparation strategy for the detection of steroid-like compounds using MALDI mass spectrometry imaging: pulmonary distribution of budesonide as a case study

2021 ◽  
Author(s):  
Riccardo Zecchi ◽  
Pietro Franceschi ◽  
Laura Tigli ◽  
Davide Amidani ◽  
Chiara Catozzi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Mass Spectrometry Imaging ◽  
Maldi Mass Spectrometry ◽  
Adult Rabbit ◽  
Chronic Obstructive ◽  
Ionization Mass ◽  
Obstructive Pulmonary Disease ◽  
Imaging Protocol ◽  
Girard’S Reagent

AbstractCorticosteroids as budesonide can be effective in reducing topic inflammation processes in different organs. Therapeutic use of budesonide in respiratory diseases, like asthma, chronic obstructive pulmonary disease, and allergic rhinitis is well known. However, the pulmonary distribution of budesonide is not well understood, mainly due to the difficulties in tracing the molecule in lung samples without the addition of a label. In this paper, we present a matrix-assisted laser desorption/ionization mass spectrometry imaging protocol that can be used to visualize the pulmonary distribution of budesonide administered to a surfactant-depleted adult rabbit. Considering that budesonide is not easily ionized by MALDI, we developed an on-tissue derivatization method with Girard’s reagent P followed by ferulic acid deposition as MALDI matrix. Interestingly, this sample preparation protocol results as a very effective strategy to raise the sensitivity towards not only budesonide but also other corticosteroids, allowing us to track its distribution and quantify the drug inside lung samples. Graphical abstract

Integrated Sample Preparation and MALDI Mass Spectrometry on a Microfluidic Compact Disk

2004 ◽  
Vol 76 (2) ◽  
pp. 345-350 ◽  
Author(s):  
Magnus Gustafsson ◽  
Daniel Hirschberg ◽  
Carina Palmberg ◽  
Hans Jörnvall ◽  
Tomas Bergman
Keyword(s):  
Mass Spectrometry ◽  
Sample Preparation ◽  
Maldi Mass Spectrometry ◽  
Compact Disk
Sign in / Sign up

Export Citation Format

Share Document