scholarly journals Real-Time DNA Quantification of Nuclear and Mitochondrial DNA in Forensic Analysis

BioTechniques ◽  
2002 ◽  
Vol 33 (2) ◽  
pp. 402-411 ◽  
Author(s):  
Hanna Andréasson ◽  
Ulf Gyllensten ◽  
Marie Allen
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Forensic Analysis ◽  
Dna Quantification

Internal Validation of Human Mitochondrial DNA Quantification Using Real-Time PCR

2014 ◽  
Vol 59 (4) ◽  
pp. 1049-1056 ◽  
Author(s):  
Marc L. Sprouse ◽  
Nicole R. Phillips ◽  
Mark F. Kavlick ◽  
Rhonda K. Roby
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Real Time Pcr ◽  
Dna Quantification ◽  
Internal Validation ◽  
Human Mitochondrial Dna

A novel method to quantify deleted mitochondrial DNA in a real time PCR

2006 ◽  
Vol 1288 ◽  
pp. 756-758 ◽  
Author(s):  
T. Schwark ◽  
C. Fisch-Kohl ◽  
N. von Wurmb-Schwark
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Real Time Pcr ◽  
Novel Method

Quantitative Detection of Species-Specific DNA in Feedstuffs and Fish Meals

2005 ◽  
Vol 68 (6) ◽  
pp. 1217-1221 ◽  
Author(s):  
PAVEL KRCMAR ◽  
EVA RENCOVA
Keyword(s):  
Mitochondrial Dna ◽  
Real Time ◽  
Dna Sequences ◽  
Real Time Pcr ◽  
Single Species ◽  
Absolute Quantification ◽  
Quantitative Detection ◽  
Vertebrate Species ◽  
Target Species ◽  
Species Specific

A sensitive and rapid method for the quantitative detection of bovine-, ovine-, swine-, and chicken-specific mitochondrial DNA sequences based on real-time PCR has been developed. The specificity of the primers and probes for real-time PCR has been tested using DNA samples of other vertebrate species that may also be present in rendered products. The quantitative detection was performed with dual-labeled probes (TaqMan) using absolute quantification with external standards of single species meat-and-bone meals. This method facilitates the detection of 0.01% of the target species–derived material in concentrate feed mixtures and fish meals.

Real-Time PCR Detection of Ruminant DNA

2004 ◽  
Vol 67 (3) ◽  
pp. 550-554 ◽  
Author(s):  
LUIS MENDOZA-ROMERO ◽  
EDWARD L. C. VERKAAR ◽  
PAUL H. SAVELKOUL ◽  
ARNOLD CATSBURG ◽  
HENK J. M. AARTS ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Bovine Spongiform Encephalopathy ◽  
Real Time ◽  
Real Time Pcr ◽  
Spongiform Encephalopathy ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Dna Targeting ◽  
Species Origin ◽  
Semiquantitative Method

To control the spread of bovine spongiform encephalopathy, several DNA methods have been described for the detection of the species origin of meat and bone meal. Most of these methods are based on the amplification of a mitochondrial DNA segment. We have developed a semiquantitative method based on real-time PCR for detection of ruminant DNA, targeting an 88-bp segment of the ruminant short interspersed nuclear element Bov-A2. This method is specific for ruminants and is able to detect as little as 10 fg of bovine DNA. Autoclaving decreased the amount of detectable DNA, but positive signals were observed in feeding stuff containing 10% bovine material if this had not been rendered in accordance with the regulations, i.e., heated at 134°C for 3 instead of 20 min.

Quantitative real-time ARMS-qPCR for mitochondrial DNA enables accurate detection of microchimerism in renal transplant recipients

2011 ◽  
Vol 15 (8) ◽  
pp. 809-818 ◽  
Author(s):  
André Hoerning ◽  
Halime Kalkavan ◽  
Christian Rehme ◽  
Julia Menke ◽  
Karl Worm ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Renal Transplant ◽  
Real Time ◽  
Renal Transplant Recipients ◽  
Transplant Recipients ◽  
Accurate Detection

scholarly journals Effects of antibiotics on Chlamydia trachomatis viability as determined by real-time quantitative PCR

2011 ◽  
Vol 60 (4) ◽  
pp. 508-514 ◽  
Author(s):  
Olivia Peuchant ◽  
Jean Philippe Duvert ◽  
Maïthé Clerc ◽  
Sophie Raherison ◽  
Christiane Bébéar ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Real Time ◽  
Dna Quantification ◽  
Mrna Transcription ◽  
Real Time Quantitative Pcr ◽  
Dna And Rna ◽  
Rna Quantification ◽  
Additional Cell

The objective of this study was to determine the effect of antibiotics on Chlamydia trachomatis viability by using a quantitative real-time PCR assay that measured DNA replication and mRNA transcription of the structural omp1 and omp2 genes, 16S rRNA and the groEL1 gene with and without antibiotics. Ofloxacin, moxifloxacin, azithromycin and doxycycline were tested against the serovar D and L2 reference strains and a derivative mutant resistant to fluoroquinolones, L2-OFXR, obtained by in vitro selection. Using DNA quantification, the antibiotic MIC was calculated when the number of DNA copies was equal to that of the chlamydial inoculum at time zero. This method allowed the easy determination of MICs by DNA quantification of the four selected genes and gave similar results to those obtained by immunofluorescence staining without biased interpretation. By using cDNA quantification, the lowest antibiotic concentration for which no RNA was transcribed corresponded to the minimum bactericidal concentration. C. trachomatis still transcribed the16S rRNA and groEL1 genes, even at concentrations well above the MIC, showing a bacteriostatic effect for all antibiotics tested. This method allows the study of antibiotic activity on growth and viability of C. trachomatis by DNA and RNA quantification at the same time without additional cell-culture passaging.

scholarly journals Common Deletion (CD) in mitochondrial DNA of irradiated rat heart

2014 ◽  
Vol 86 (2) ◽  
pp. 685-694 ◽  
Author(s):  
RAQUEL G. SIQUEIRA ◽  
DAYSE A. DA SILVA ◽  
LUIZ D.B. DE MELO ◽  
ELIZEU F. DE CARVALHO ◽  
SAMARA C. FERREIRA-MACHADO ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Ionizing Radiation ◽  
Real Time ◽  
Real Time Pcr ◽  
Rat Heart ◽  
Wistar Rats ◽  
Post Irradiation ◽  
Male Wistar Rats ◽  
The Common ◽  
Common Deletion

The purpose of this study was to map the common deletion (CD) area in mtDNA and investigate the levels of this deletion in irradiated heart. The assays were developed in male Wistar rats that were irradiated with three different single doses (5, 10 or 15 Gy) delivered directly to the heart and the analyses were performed at various times post-irradiation (3, 15 or 120 days). The CDs area were sequenced and the CD quantified by real-time PCR. Our study demonstrated that the CD levels progressively decreased from the 3rduntil the 15th day after irradiation, and then increased thereafter. Additionally, it was observed that the levels of CD are modulated differently according to the different categories of doses (moderate and high). This study demonstrated an immediate response to ionizing radiation, measured by the presence of mutations in the CD area and a decrease in the CD levels.

Sign in / Sign up

Export Citation Format

Share Document