scholarly journals One-Step, Highly Efficient Site-Directed Mutagenesis by Toxic Protein Selection

BioTechniques ◽  
2002 ◽  
Vol 32 (6) ◽  
pp. 1266-1270 ◽  
Author(s):  
W. Xu ◽  
Y. Zhang ◽  
L.-Y. Yeh ◽  
C.R. Ruprecht ◽  
F. Wong-Staal ◽  
...  
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Toxic Protein ◽  
Highly Efficient ◽  
One Step ◽  
Protein Selection

One-step site-directed mutagenesis of viroid dimeric cDNA

2007 ◽  
Vol 145 (1) ◽  
pp. 71-75 ◽  
Author(s):  
Rafael Sanjuán ◽  
José-Antonio Daròs
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
One Step ◽  
Step Site

One-Step site-directed mutagenesis of ATM cDNA in large (20kb) plasmid constructs

2002 ◽  
Vol 20 (4) ◽  
pp. 323-323 ◽  
Author(s):  
Shaun P. Scott ◽  
Alison Teh ◽  
Cheng Peng ◽  
Martin F. Lavin
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
One Step ◽  
Step Site

A Novel PCR Site-Directed Mutagenesis to Modify Structure of Erabutoxin B

2011 ◽  
Vol 183-185 ◽  
pp. 605-610
Author(s):  
Fang Hui Wu ◽  
Yan Jun Li ◽  
Ting Song Yang ◽  
Tian Yi Ying
Keyword(s):  
Primary Structure ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Function Structure ◽  
One Step ◽  
Ld50 Value ◽  
The One ◽  
Erabutoxin B ◽  
Structure Of Proteins ◽  
Modify Structure

Objective: A convenient PCR was used for site-directed mutagenesis to modify structure of proteins or peptides, For function-structure studies of proteins or peptides. Method: the one-step PCR site-directed mutagenesis strategy can introduce mutation of gene through 5/-end of primer, which was applied on peptide Erabutoxin B (EB) to produce three mutants of EB, the activity of mutants was detected by LD50 value of mice. Result: S8Y, R33D, K47R three mutants of EB were obtained by one-step PCR, LD50 of mutants indicated that the activity of mutants decreased in different degree, the activity of R33D was nearly deprived. Conclusion: one-step PCR site-directed mutagenesis was convenien and efficient, it can be applied on restructuring the primary structure of proteins or peptides.

scholarly journals Isolation of Cry1Ab protein mutants ofBacillus thuringiensisby a highly efficient PCR site-directed mutagenesis system

1996 ◽  
Vol 145 (3) ◽  
pp. 333-339 ◽  
Author(s):  
Roberto Meza ◽  
Maria-Eugenia Nuñez-Valdez ◽  
Jorge Sanchez ◽  
Alejandra Bravo
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Cry1ab Protein ◽  
Highly Efficient ◽  
Protein Mutants

scholarly journals REPLACR-mutagenesis, a one-step method for site-directed mutagenesis by recombineering

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Ashutosh Trehan ◽  
Michał Kiełbus ◽  
Jakub Czapinski ◽  
Andrzej Stepulak ◽  
Ilpo Huhtaniemi ◽  
...  
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Step Method ◽  
One Step

One-step site-directed mutagenesis of the Kex2 protease oxyanion hole

1993 ◽  
Vol 3 (8) ◽  
pp. 498-506 ◽  
Author(s):  
Charles Brenner ◽  
Alison Bevan ◽  
Robert S. Fuller
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Oxyanion Hole ◽  
One Step ◽  
Kex2 Protease ◽  
Step Site
Sign in / Sign up

Export Citation Format

Share Document