scholarly journals Peptide Nucleic Acid Probe Detection of Mutations in Mycobacterium tuberculosis Genes Associated with Drug Resistance

BioTechniques ◽  
2002 ◽  
Vol 32 (3) ◽  
pp. 508-514 ◽  
Author(s):  
L.E. Bockstahler ◽  
Z. Li ◽  
N.Y. Nguyen ◽  
K.A. Van Houten ◽  
M.J. Brennan ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Probe Detection ◽  
Peptide Nucleic Acid Probe ◽  
Detection Of Mutations

scholarly journals Characterization of Mutations Conferring Extensive Drug Resistance to Mycobacterium tuberculosis Isolates in Pakistan

2011 ◽  
Vol 55 (12) ◽  
pp. 5654-5659 ◽  
Author(s):  
Asho Ali ◽  
Rumina Hasan ◽  
Kauser Jabeen ◽  
Nusrat Jabeen ◽  
Ejaz Qadeer ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mycobacterium Tuberculosis ◽  
Hot Spot ◽  
East African ◽  
Content Type ◽  
Diagnostic Assays ◽  
Asian Strain ◽  
Central Asian ◽  
Extensive Drug Resistance ◽  
Detection Of Mutations

ABSTRACTThe increasing incidence of extensively drug-resistant (XDR)Mycobacterium tuberculosisin high-tuberculosis-burden countries further highlights the need for improved rapid diagnostic assays. An increasing incidence of XDRM. tuberculosisstrains in Pakistan has been reported, but drug resistance-associated mutations in these strains have not been evaluated previously. We sequenced the “hot-spot” regions ofrpoB,katG,inhA,ahpC,gyrA,gyrB, andrrsgenes in 50 XDRM. tuberculosisstrains. It was observed that 2% of rifampin, 6% of isoniazid, 24% of fluoroquinolone, and 32% of aminoglycoside/capreomycin resistance in XDRM. tuberculosisstrains would be undetected if only these common hot-spot regions were tested. The frequencies of resistance-conferring mutations were found to be comparable among all XDRM. tuberculosisstrain families present, including the Central Asian Strain, Beijing, and East African Indian genogroups and the Unique isolates. Additional genetic loci need to be tested for detection of mutations conferring fluoroquinolone, aminoglycoside, and capreomycin resistance in order to improve molecular diagnosis of regional XDRM. tuberculosisstrains.

scholarly journals Rapid Identification of Staphylococcus aureus and Methicillin Resistance by Flow Cytometry Using a Peptide Nucleic Acid Probe

2011 ◽  
Vol 49 (9) ◽  
pp. 3383-3385 ◽  
Author(s):  
Nabin K. Shrestha ◽  
Nikole M. Scalera ◽  
Deborah A. Wilson ◽  
Byron Brehm-Stecher ◽  
Gary W. Procop
Keyword(s):  
Staphylococcus Aureus ◽  
Flow Cytometry ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Methicillin Resistance ◽  
Rapid Identification ◽  
Peptide Nucleic Acid Probe

Detection of glyceraldehyde 3-phosphate dehydrogenase messenger RNA using a peptide nucleic acid probe in paraffin-embedded archival specimens

2004 ◽  
Vol 54 (4) ◽  
pp. 251-255 ◽  
Author(s):  
Makoto Hiroyasu ◽  
Shinya Akatsuka ◽  
Tomoyuki Shirase ◽  
Yoshinobu Toda ◽  
Hiroshi Hiai ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Messenger Rna ◽  
Peptide Nucleic Acid Probe ◽  
Archival Specimens

scholarly journals Identification of pathogenic bacteria in complex samples using a smartphone based fluorescence microscope

RSC Advances ◽  
2018 ◽  
Vol 8 (64) ◽  
pp. 36493-36502 ◽  
Author(s):  
Vilhelm Müller ◽  
José M. Sousa ◽  
Hatice Ceylan Koydemir ◽  
Muhammed Veli ◽  
Derek Tseng ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Pathogenic Bacteria ◽  
Fluorescence Microscope ◽  
Complex Samples ◽  
Peptide Nucleic Acid Probe ◽  
Species Specific ◽  
Specific Peptide

A smartphone-based fluorescence microscope for detection of bacteria in complex samples using a species-specific peptide nucleic acid probe.

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