Detection of Helicobacter pylori with clarithromycin resistance‐associated mutations using peptide nucleic acid probe‐based melting point analysis

Helicobacter ◽  
2019 ◽  
Vol 24 (5) ◽  
Author(s):  
Ji Hae Nahm ◽  
Won Kyu Kim ◽  
Yujin Kwon ◽  
Hyunki Kim
Keyword(s):  
Helicobacter Pylori ◽  
Melting Point ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Peptide Nucleic Acid Probe ◽  
Clarithromycin Resistance ◽  
Melting Point Analysis ◽  
Point Analysis

scholarly journals Peptide Nucleic Acid Probe-Based Analysis as a New Detection Method for Clarithromycin Resistance inHelicobacter pylori

Gut and Liver ◽  
2018 ◽  
Vol 12 (6) ◽  
pp. 641-647 ◽  
Author(s):  
Da Hyun Jung ◽  
Jie-Hyun Kim ◽  
Su Jin Jeong ◽  
Soon Young Park ◽  
Il-Mo Kang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Detection Method ◽  
Peptide Nucleic Acid Probe ◽  
Clarithromycin Resistance

scholarly journals Rapid and Reliable Method for Cytochrome P450 2D6 Genotyping

2002 ◽  
Vol 48 (9) ◽  
pp. 1412-1417 ◽  
Author(s):  
Ulrike M Stamer ◽  
Bettina Bayerer ◽  
Stephanie Wolf ◽  
Andreas Hoeft ◽  
Frank Stüber
Keyword(s):  
Melting Point ◽  
Real Time ◽  
Real Time Pcr ◽  
Reliable Method ◽  
Cytochrome P450 2D6 ◽  
Cyp2d6 Gene ◽  
Melting Point Analysis ◽  
Point Analysis ◽  
Allele Specific ◽  
P450 2D6

Abstract Background: Single-nucleotide polymorphisms and single-base deletions within the cytochrome P450 2D6 (CYP2D6) gene have been associated with a poor metabolizer (PM) phenotype and display a frequency of 7–10% in the Caucasian population. Methods: We developed a reliable and rapid procedure to identify five major PM-associated mutations (CYP2D6*4, *7, and *8) and deletions (CYP2D6*3 and *6) by real-time PCR with subsequent fluorometric melting point analysis of the PCR product. These polymorphisms within the CYP2D6 gene were detected by use of two primer pairs and five different pairs of hybridization probes. DNA extracted from whole blood of 323 individuals was analyzed, and results were compared with genotypes obtained by allele-specific multiplex PCR. In case of uncertain results, additional sequence analysis was performed. Results: Genotyping results by real-time PCR were 100% reliable, whereas conventional allele-specific multiplex PCR produced uncertain results for 12.1% of samples, as confirmed by sequence analysis. Costs for reagents and consumables were considerably higher for the real-time PCR technology, but labor time was reduced by 2 h compared with allele-specific PCR. The allele frequencies in the population investigated were 0.186 for allele *4, 0.026 for allele *5, 0.009 for allele *3, 0.031 for allele *6, and 0.002 for allele *8. The defective CYP2D6*7 allele was not found. In addition, three additional mutations were detected, one of them displaying a PM genotype. Conclusion: Genotyping of CYP2D6 by real-time PCR with fluorometric melting point analysis is a rapid and reliable method.

scholarly journals Rapid Identification of Staphylococcus aureus and Methicillin Resistance by Flow Cytometry Using a Peptide Nucleic Acid Probe

2011 ◽  
Vol 49 (9) ◽  
pp. 3383-3385 ◽  
Author(s):  
Nabin K. Shrestha ◽  
Nikole M. Scalera ◽  
Deborah A. Wilson ◽  
Byron Brehm-Stecher ◽  
Gary W. Procop
Keyword(s):  
Staphylococcus Aureus ◽  
Flow Cytometry ◽  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Methicillin Resistance ◽  
Rapid Identification ◽  
Peptide Nucleic Acid Probe
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