scholarly journals A simple high-throughput technology enables gain-of-function screening of human microRNAs

BioTechniques ◽  
2013 ◽  
Vol 54 (2) ◽  
Author(s):  
Wen-Chih Cheng ◽  
Tami J. Kingsbury ◽  
Sarah J. Wheelan ◽  
Curt I. Civin
Catalysis ◽  
2013 ◽  
pp. 172-215 ◽  
Author(s):  
Stephan A. Schunk ◽  
Natalia Böhmer ◽  
Cornelia Futter ◽  
Andreas Kuschel ◽  
Eko Prasetyo ◽  
...  

2011 ◽  
Vol 12 (5) ◽  
pp. 054101 ◽  
Author(s):  
Jonas Loskyll ◽  
Klaus Stoewe ◽  
Wilhelm F Maier

2013 ◽  
Vol 22 (2) ◽  
pp. 330-339 ◽  
Author(s):  
Patrick C. Baer ◽  
Selim Kuçi ◽  
Michael Krause ◽  
Zyrafete Kuçi ◽  
Stefan Zielen ◽  
...  

2003 ◽  
Vol 43 (3) ◽  
pp. 146-149
Author(s):  
Mitsuaki SUGAHARA ◽  
Masaki YAMAMOTO ◽  
Masashi MIYANO

2015 ◽  
Vol 9 (1) ◽  
pp. 210-215
Author(s):  
Xiaojun Kang ◽  
Cheng Yang ◽  
Xuguang Zhao ◽  
Weiwei Chen ◽  
Sifa Zhang ◽  
...  

Current genome sequencing techniques are expensive, and it is still a major challenge to obtain an individual whole-genome sequence. To reduce the cost of sequencing, this paper introduced a high-throughput sequencing strategy using a three-dimensional mixing-pools based on the cube. Following the strategy, BAC clones were injected into each vertex of the cube, and sequencing of each plane provided information about multiple clones, thereby significantly reducing the cost of sequencing. In addition, Velvet was used to assemble the sequencing data. The scaffold generated from Velvet contained a number of contigs, which were orderless. Therefore, to address this problem, a scaffold assembly algorithm based on multi-way trees was used. The algorithm used a multi-way tree to build the framework of chromosomes, and subsequently, the frame was filled to complete the scaffold assembly. This algorithm alone outperformed Velvet in the assembling of a scaffold.


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