scholarly journals S10H3 Utilization of X-ray free electron laser in biophysics(State-of-the-art Techniques to Build the Coming Generation in Biophysics: Novel Approaches to Revealing Molecular Mechanisms of Cells and Proteins)

2007 ◽  
Vol 47 (supplement) ◽  
pp. S14
Author(s):  
Masayoshi Nakasako
Keyword(s):  
Free Electron ◽  
Free Electron Laser ◽  
Molecular Mechanisms ◽  
State Of The Art ◽  
X Ray ◽  
Novel Approaches ◽  
Art Techniques

scholarly journals Structural studies of P-type ATPase–ligand complexes using an X-ray free-electron laser

IUCrJ ◽  
2015 ◽  
Vol 2 (4) ◽  
pp. 409-420 ◽  
Author(s):  
Maike Bublitz ◽  
Karol Nass ◽  
Nikolaj D. Drachmann ◽  
Anders J. Markvardsen ◽  
Matthias J. Gutmann ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Structure Determination ◽  
Free Electron ◽  
Free Electron Laser ◽  
Molecular Mechanisms ◽  
Structural Information ◽  
Biologically Active ◽  
Atpase Inhibitor ◽  
X Ray ◽  
P Type

Membrane proteins are key players in biological systems, mediating signalling events and the specific transport ofe.g.ions and metabolites. Consequently, membrane proteins are targeted by a large number of currently approved drugs. Understanding their functions and molecular mechanisms is greatly dependent on structural information, not least on complexes with functionally or medically important ligands. Structure determination, however, is hampered by the difficulty of obtaining well diffracting, macroscopic crystals. Here, the feasibility of X-ray free-electron-laser-based serial femtosecond crystallography (SFX) for the structure determination of membrane protein–ligand complexes using microcrystals of various native-source and recombinant P-type ATPase complexes is demonstrated. The data reveal the binding sites of a variety of ligands, including lipids and inhibitors such as the hallmark P-type ATPase inhibitor orthovanadate. By analyzing the resolution dependence of ligand densities and overall model qualities, SFX data quality metrics as well as suitable refinement procedures are discussed. Even at relatively low resolution and multiplicity, the identification of ligands can be demonstrated. This makes SFX a useful tool for ligand screening and thus for unravelling the molecular mechanisms of biologically active proteins.

scholarly journals Undulator beamline optimization with integrated chicanes for X-ray free-electron-laser facilities

2016 ◽  
Vol 23 (4) ◽  
pp. 861-868 ◽  
Author(s):  
Eduard Prat ◽  
Marco Calvi ◽  
Romain Ganter ◽  
Sven Reiche ◽  
Thomas Schietinger ◽  
...  
Keyword(s):  
Numerical Simulations ◽  
High Power ◽  
Free Electron ◽  
Free Electron Laser ◽  
State Of The Art ◽  
X Ray ◽  
Higher Power ◽  
Longitudinal Coherence

An optimization of the undulator layout of X-ray free-electron-laser (FEL) facilities based on placing small chicanes between the undulator modules is presented. The installation of magnetic chicanes offers the following benefits with respect to state-of-the-art FEL facilities: reduction of the required undulator length to achieve FEL saturation, improvement of the longitudinal coherence of the FEL pulses, and the ability to produce shorter FEL pulses with higher power levels. Numerical simulations performed for the soft X-ray beamline of the SwissFEL facility show that optimizing the advantages of the layout requires shorter undulator modules than the standard ones. This proposal allows a very compact undulator beamline that produces fully coherent FEL pulses and it makes possible new kinds of experiments that require very short and high-power FEL pulses.

X-Ray Free Electron Laser Facility for Next Generation of Scientific Exploration and Discovery

2011 ◽  
Vol 131 (2) ◽  
pp. 68-71
Author(s):  
Etsuo FUJIWARA ◽  
Eiichi ANAYAMA ◽  
Yuichiro KATSUTA ◽  
Toshiki IZUTANI ◽  
Daichi OKUHARA ◽  
...  
Keyword(s):  
Free Electron ◽  
Free Electron Laser ◽  
Next Generation ◽  
X Ray ◽  
Scientific Exploration ◽  
Laser Facility

X-ray Free Electron Laser SACLA

2014 ◽  
Vol 134 (12) ◽  
pp. 836-839
Author(s):  
Junichi INOUE ◽  
Yuji TANAKA ◽  
Yuki MATSUMOTO ◽  
Kensuke KANDA
Keyword(s):  
Free Electron ◽  
Free Electron Laser ◽  
X Ray
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