scholarly journals Optical measurement of action potential. Multiple-site optical recording.

1986 ◽  
Vol 26 (3) ◽  
pp. 138-143
Author(s):  
KOTARO KAMINO
Keyword(s):  
Action Potential ◽  
Optical Measurement ◽  
Optical Recording ◽  
Multiple Site

scholarly journals Characterization of impulse propagation at the microscopic level across geometrically defined expansions of excitable tissue: multiple site optical recording of transmembrane voltage (MSORTV) in patterned growth heart cell cultures.

1994 ◽  
Vol 104 (2) ◽  
pp. 287-309 ◽  
Author(s):  
S Rohr ◽  
B M Salzberg
Keyword(s):  
Action Potential ◽  
Optical Recording ◽  
Neonatal Rat ◽  
Two Dimensional ◽  
Multiple Site ◽  
Patterned Growth ◽  
Excitable Tissue ◽  
Impulse Propagation ◽  
Transmembrane Voltage ◽  
Narrow Cell

Impulse propagation across sudden expansions of excitable tissue has been shown to exhibit various forms of conduction disturbance on a macroscopic scale, ranging from small delays to unidirectional or complete conduction block. With the present study, we attempted to characterize systematically the dependence of impulse propagation on the geometry of the underlying excitable tissue on a microscopic scale by investigating the spatio-temporal pattern of transmembrane voltage changes associated with impulse propagation from a narrow cell strand to a large cell area using multiple site optical recording of transmembrane voltage (MSORTV) in conjunction with patterned growth of neonatal rat heart cells in culture. While action potential propagation was smooth in the case of funneled expansions, delays of variable size occurred during propagation into rectangular or incised expansions. Close to the abrupt expansion, which functionally represented an increased electrical load to the narrow cell strand, the delays were accompanied by marked distortions of the action potential upstroke, exhibiting, in extreme cases, an initial depolarization to 50% followed by a delayed secondary depolarization to 100% of the full-signal amplitude. These distortions, which were based on bidirectional electrotonic interactions across the transition, were maximal immediately downstream from the expansion. The maximal slowing of impulse conduction across abrupt expansions was, in agreement with recently published results obtained from two-dimensional computer simulations, always situated in the expanded region. At high stimulation rates, the delays sometimes turned into intermittent unidirectional blocks, as revealed by reverse stimulation. These blocks were always characterized by a marked abbreviation of the action potentials upstream from the region causing the block which might, in an appropriate network, facilitate reentry because of the associated shortening of the refractory period. Because the patterns were composed of cells having identical membrane properties, the results show that the local action potential shape can be modulated profoundly by the two-dimensional architecture of the underlying cell ensemble alone.

scholarly journals Optical Measurement of Action Potential in Adult Ventricular Myocytes

2011 ◽  
Vol 100 (3) ◽  
pp. 292a ◽  
Author(s):  
Qinghai Tian ◽  
Martin Oberhofer ◽  
Sandra Ruppenthal ◽  
Anke Scholz ◽  
Volker Buschmann ◽  
...  
Keyword(s):  
Action Potential ◽  
Ventricular Myocytes ◽  
Optical Measurement

scholarly journals Multiple-site optical recording of membrane potential from a salivary gland. Interaction of synaptic and electrotonic excitation.

1983 ◽  
Vol 81 (6) ◽  
pp. 887-908 ◽  
Author(s):  
D M Senseman ◽  
H Shimizu ◽  
I S Horwitz ◽  
B M Salzberg
Keyword(s):  
Salivary Gland ◽  
Electronic Excitation ◽  
Electrical Coupling ◽  
Longitudinal Direction ◽  
Optical Recording ◽  
Multiple Site ◽  
Neuronal Stimulation ◽  
High Degree ◽  
Matrix Arrays ◽  
Stimulation Of

The interaction between synaptic and electronic excitation of cells from the salivary gland of the snail Helisoma trivolvis was studied using a voltage-sensitive merocyanine dye. Linear and square photodiode matrix arrays were used to record simultaneously the response to neuronal stimulation of 15-25 separate regions of the gland. Laterally opposed acini exhibited highly synchronous electrical activity, which suggested a correspondingly high degree of electrical coupling. In the longitudinal direction, coupling appeared weaker. The onset of depolarization after neuronal stimulation was progressively delayed along the longitudinal gland axis, in agreement with the measured conduction velocity of the presynaptic nerve spike. In most instances, neuronal stimulation directly activated a regenerative gland response (action potential) at the junction between the anterior and central duct. Excitation of distal gland regions was usually mediated by electronic spread from active, more proximal gland regions. Occasionally, "collisions" between excitatory waves traveling in opposite directions were observed.

Optical Recording of Action Potentials and Other Discrete Physiological Events: A Perspective from Signal Detection Theory

Physiology ◽  
2007 ◽  
Vol 22 (1) ◽  
pp. 47-55 ◽  
Author(s):  
Lucas Sjulson ◽  
Gero Miesenböck
Keyword(s):  
Action Potential ◽  
Signal Detection ◽  
Action Potentials ◽  
Signal Detection Theory ◽  
Biological Function ◽  
Optical Recording ◽  
Trial Data ◽  
Single Trial ◽  
Optical Reporters ◽  
Potential Recording

Optical imaging of physiological events in real time can yield insights into biological function that would be difficult to obtain by other experimental means. However, the detection of all-or-none events, such as action potentials or vesicle fusion events, in noisy single-trial data often requires a careful balance of tradeoffs. The analysis of such experiments, as well as the design of optical reporters and instrumentation for them, is aided by an understanding of the principles of signal detection. This review illustrates these principles, using as an example action potential recording with optical voltage reporters.

Processing of multiple-site optical measurement data

2000 ◽  
Vol 67 (7) ◽  
pp. 634
Author(s):  
V. F. Fateev ◽  
A. P. Aleshkin
Keyword(s):  
Optical Measurement ◽  
Measurement Data ◽  
Multiple Site
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