scholarly journals Depletion of Primordial Germ Cells (PGCs) by X-irradiation to Extraembryonic Region of Chicken Embryos and Expression of Xenotransplanted Quail PGCs

2009 ◽  
Vol 46 (2) ◽  
pp. 136-143 ◽  
Author(s):  
Yusuke Atsumi ◽  
Shigenobu Yazawa ◽  
Fumitake Usui ◽  
Yoshiaki Nakamura ◽  
Yasuhiro Yamamoto ◽  
...  
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Chicken Embryos ◽  
X Irradiation

scholarly journals In Vivo Transfer of Foreign DNA into Primordial Germ Cells (PGCs) of Chicken Embryos

1999 ◽  
Vol 12 (4) ◽  
pp. 520-524 ◽  
Author(s):  
K. Eguma ◽  
T. Soh ◽  
M. Hattori ◽  
N. Fujihara
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Chicken Embryos ◽  
Foreign Dna

Increased proportion of donor primordial germ cells in chimeric gonads by sterilisation of recipient embryos using busulfan sustained-release emulsion in chickens

2008 ◽  
Vol 20 (8) ◽  
pp. 900 ◽  
Author(s):  
Yoshiaki Nakamura ◽  
Yasuhiro Yamamoto ◽  
Fumitake Usui ◽  
Yusuke Atsumi ◽  
Yohei Ito ◽  
...  
Keyword(s):  
Sustained Release ◽  
Germ Cell ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Primordial Germ Cell ◽  
Sesame Oil ◽  
Chicken Embryos ◽  
Whole Mount ◽  
Phosphate Buffered Saline ◽  
Transfer Study

The aim of the present study was to improve the efficiency of endogenous primordial germ cell (PGC) depletion and to increase the ratio of donor PGCs in the gonads of recipient chicken embryos. A sustained-release emulsion was prepared by emulsifying equal amounts of Ca2+- and Mg2+-free phosphate-buffered saline containing 10% busulfan solubilised in N,N-dimethylformamide and sesame oil, using a filter. Then, 75 μg per 50 μL busulfan sustained-release emulsion was injected into the yolk. To determine the depletion and repopulation of PGCs in the gonads after 6 days incubation, whole-mount immunostaining was performed. The busulfan sustained-release emulsion significantly reduced the number of endogenous PGCs compared with control (P < 0.05). Moreover, the busulfan sustained-release emulsion significantly depleted endogenous PGCs compared with other previously reported busulfan delivery systems (P < 0.05), but with less variation, suggesting that the sustained-release emulsion delivered a consistent amount of busulfan to the developing chicken embryos. The PGC transfer study showed that the proportion of donor PGCs in the gonads of busulfan sustained-release emulsion-treated embryos after 6 days incubation increased 28-fold compared with control. In conclusion, the results demonstrate that exogenous PGCs are capable of migrating and settling in gonads from which endogenous PGCs have been removed using a busulfan sustained-release emulsion.

scholarly journals Knockdown of DEAD-box helicase 4 (DDX4) decreases the number of germ cells in male and female chicken embryonic gonads

2019 ◽  
Vol 31 (5) ◽  
pp. 847
Author(s):  
Nana Aduma ◽  
Hiroe Izumi ◽  
Shusei Mizushima ◽  
Asato Kuroiwa
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Retroviral Vectors ◽  
Ovary Development ◽  
Aromatase Expression ◽  
Male And Female ◽  
Chicken Embryos ◽  
Dead Box ◽  
Related Transcription Factor ◽  
Cytochrome P450 Family

DEAD-box helicase 4 (DDX4; also known as vasa) is essential for the proper formation and maintenance of germ cells. Although DDX4 is conserved in a variety of vertebrates and invertebrates, its roles differ between species. This study investigated the function of DDX4 in chicken embryos by knocking down its expression using retroviral vectors that encoded DDX4-targeting microRNAs. DDX4 was effectively depleted invitro and invivo via this approach. Male and female gonads of DDX4-knockdown embryos contained a decreased number of primordial germ cells, indicating that DDX4 is essential to maintain a normal level of these cells in chicken embryos of both sexes. Expression of doublesex and mab-3 related transcription factor 1 (DMRT1) and sex determining region Y-box 9 (SOX9), which are involved in testis determination and differentiation, was normal in male gonads of DDX4-knockdown embryos. In contrast, expression of cytochrome P450 family 19 subfamily A member 1 (CYP19A1), which encodes aromatase and is essential for ovary development, was significantly decreased in female gonads of DDX4-knockdown embryos. Expression of forkhead box L2 (FOXL2), which plays an important role in ovary differentiation, was also slightly reduced in DDX4-knockdown embryos, but not significantly. Based on several pieces of evidence FOXL2 was hypothesised to regulate aromatase expression. The results of this study indicate that aromatase expression is also regulated by several additional pathways.

scholarly journals Intense Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Circulating Primordial Germ Cells in vitro and in vivo

2007 ◽  
Vol 44 (4) ◽  
pp. 416-425 ◽  
Author(s):  
Mitsuru Naito ◽  
Takeo Minematsu ◽  
Takashi Harumi ◽  
Takashi Kuwana
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Chicken Embryos ◽  
Gfp Gene

scholarly journals Foreign Gene Expression in the Gonads of Chimaeric Chicken Embryos by Transfer of Primordial Germ Cells Transfected in vitro by Lipofection for 24 Hours

2000 ◽  
Vol 71 (3) ◽  
pp. 308-311
Author(s):  
Mitsuru NAITO ◽  
Yuko MATSUBARA ◽  
Takashi HARUMI ◽  
Takahiro TAGAMI ◽  
Michiharu SAKURAI ◽  
...  
Keyword(s):  
Gene Expression ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Foreign Gene ◽  
Foreign Gene Expression ◽  
Chicken Embryos

scholarly journals A Drosophila toolkit for defining gene function in spermatogenesis

Reproduction ◽  
2017 ◽  
Vol 153 (4) ◽  
pp. R121-R132 ◽  
Author(s):  
N A Siddall ◽  
G R Hime
Keyword(s):  
Germ Cells ◽  
Gene Function ◽  
Primordial Germ Cells ◽  
Rapid Assessment ◽  
Model Organism ◽  
Time Frame ◽  
Individual Organism ◽  
Expression Of Genes ◽  
X Irradiation ◽  
Human Spermatogenesis

Expression profiling and genomic sequencing methods enable the accumulation of vast quantities of data that relate to the expression of genes during the maturation of male germ cells from primordial germ cells to spermatozoa and potential mutations that underlie male infertility. However, the determination of gene function in specific aspects of spermatogenesis or linking abnormal gene function with infertility remain rate limiting, as even in an era of CRISPR analysis of gene function in mammalian models, this still requires considerable resources and time. Comparative developmental biology studies have shown the remarkable conservation of spermatogenic developmental processes from insects to vertebrates and provide an avenue of rapid assessment of gene function to inform the potential roles of specific genes in rodent and human spermatogenesis. The vinegar fly, Drosophila melanogaster, has been used as a model organism for developmental genetic studies for over one hundred years, and research with this organism produced seminal findings such as the association of genes with chromosomes, the chromosomal basis for sexual identity, the mutagenic properties of X-irradiation and the isolation of the first tumour suppressor mutations. Drosophila researchers have developed an impressive array of sophisticated genetic techniques for analysis of gene function and genetic interactions. This review focuses on how these techniques can be utilised to study spermatogenesis in an organism with a generation time of 9 days and the capacity to introduce multiple mutant alleles into an individual organism in a relatively short time frame.

Laser irradiation of the chick embryo germinal crescent

Development ◽  
1971 ◽  
Vol 26 (1) ◽  
pp. 31-36
Author(s):  
Martha Fearon Mims ◽  
Robert Gilmore McKinnell
Keyword(s):  
Chick Embryo ◽  
Laser Irradiation ◽  
Germ Cells ◽  
Ruby Laser ◽  
Primordial Germ Cells ◽  
Chicken Embryos ◽  
Somite Stage ◽  
Multiple Pulses

The germinal crescent of head-fold and one-somite-stage chicken embryos was irradiated with multiple pulses of a microbeam ruby laser. Primordial germ cells were not detected in the gonad primordium of six laser-irradiated 5-day embryos; ten laser-irradiated embryos had varying numbers of primordial germ cells. Ten control embryos had gonad primordia populated with many primordial germ cells.

scholarly journals Concentration and total number of circulating primordial germ cells in Green-legged Partridgelike chicken embryos

2021 ◽  
Vol 100 (1) ◽  
pp. 319-324
Author(s):  
Agata Szczerba ◽  
Takashi Kuwana ◽  
Marek Bednarczyk
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Chicken Embryos
Sign in / Sign up

Export Citation Format

Share Document