scholarly journals Protein utilization and amino acid digestibility of some leaf protein concentrates supplemented with amino acids in chickens.

1984 ◽  
Vol 21 (2) ◽  
pp. 89-93
Author(s):  
Suwit TERAPUNTUWAT ◽  
Iwao TASAKI
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Leaf Protein ◽  
Protein Utilization ◽  
Protein Concentrates

scholarly journals High throughput method development and optimised production of leaf protein concentrates with potential to support the agri-industry

2021 ◽  
Author(s):  
Ajay Iyer ◽  
Lisa Guerrier ◽  
Salomé Leveque ◽  
Charles S. Bestwick ◽  
Sylvia H. Duncan ◽  
...  
Keyword(s):  
Amino Acid ◽  
High Throughput ◽  
High Throughput Screening ◽  
Protein Extraction ◽  
Leaf Protein ◽  
Protein Recovery ◽  
Screening Methods ◽  
Extraction Technology ◽  
Protein Concentrates ◽  
Ethanol Precipitation

AbstractInvasive plants offer an interesting and unconventional source of protein and the considerable investment made towards their eradication can potentially be salvaged through their revalorisation. To identify viable sources, effective and high-throughput screening methods are required, as well as efficient procedures to isolate these components. Rigorous assessment of low-cost, high-throughput screening assays for total sugar, phenolics and protein was performed, and ninhydrin, Lever and Fast Blue assays were found to be most suitable owing to high reliability scores and false positive errors less than 1%. These assays were used to characterise invasive Scottish plants such as Gorse (Ulex europeans), Broom (Cystisus scoparius) and Fireweed (Chamaenerion angustifolium). Protein extraction (alkali-, heat- and enzyme assisted) were tested on these plants, and further purification (acid and ethanol precipitation, as well as ultrafiltration) procedures were tested on Gorse, based on protein recovery values. Cellulase treatment and ethanol precipitation gave the highest protein recovery (64.0 ± 0.5%) and purity (96.8 ± 0.1%) with Gorse. The amino acid profile of the purified protein revealed high levels of essential amino acids (34.8 ± 0.0%). Comparison of results with preceding literature revealed a strong association between amino acid profiles and overall protein recovery with the extraction method employed. The final purity of the protein concentrates was closely associated to the protein content of the initial plant mass. Leaf protein extraction technology can effectively raise crop harvest indices, revalorise underutilised plants and waste streams.

EFFECT OF STEAM PELLETING AND REGRINDING ON THE DIGESTIBILITY OF PROTEIN IN CEREAL GRAINS, SOYBEAN MEAL AND WHEAT BRAN BY THE RAT

1968 ◽  
Vol 48 (1) ◽  
pp. 35-39 ◽  
Author(s):  
E. M. Olsen ◽  
S. J. Slinger
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Wheat Bran ◽  
Soybean Meal ◽  
Protein Digestibility ◽  
Essential Amino Acids ◽  
Cereal Grains ◽  
Protein Utilization ◽  
Amino Acid Availability ◽  
Net Protein

The effect if steam pelleting and regrinding on digestibility of protein in corn, wheat, barley, oats, soybean meal and wheat bran was tested with rats. Percentage amino acid absorption and net protein utilization (NPU) were determined for the wheat bran. Pelleting and regrinding improved the digestibility of protein in bran but had no effect on the digestibility of protein in the other ingredients tested. Increased absorption of amino acids caused by the increased digestibility of protein in bran varied considerably for individual amino acids, being greatest for isoleucine, lysine, methionine and threonine of the essential amino acids. The improvement in protein digestibility and amino acid availability was reflected in a higher NPU.

scholarly journals Non-essential nitrogen and protein utilization in the growing rat

1990 ◽  
Vol 64 (3) ◽  
pp. 653-661 ◽  
Author(s):  
J. Heger
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Essential Amino Acid ◽  
Essential Amino Acids ◽  
Protein Utilization ◽  
Biological Value ◽  
Growing Rats ◽  
Growing Rat ◽  
Per Se

Three series of nitrogen-balance experiments were carried out on growing rats fed on purified isonitrogenous diets (16 g N/kg) to study the importance of non-essential N and the essential:total N (E:T) ratio for attaining maximum N balance (NB) and biological value (BV) of protein. Minimum dietary levels of asparagine, proline and glutamic acid required for maximum NB and BV were estimated to be 1.0, 2.0 and 5.0 g/kg respectively. In an essential amino acid-based diet, the levels of individual amino acids were successively reduced to 110% of the requirement. Reducing the level of arginine, lysine or methionine + cystine resulted in a significant increase in NB and BV while the response of rats given the isoleucine-reduced diet significantly decreased. Addition of asparagine, proline and glutamic acid in the estimated minimum amounts to an essential amino acid-based diet resulted in a significant increase in NB and BV. A further significant increase was found when the levels of arginine, lysine and methionine + cystine in the diet were reduced to 110 % of the requirement. The performance of rats fed on the latter diet was similar to that of rats given a diet with the optimum E:T ratio. It is concluded that the optimum protein utilization may be influenced by the presence of some non-essential amino acids and by the surplus of some essential amino acids rather than by the E:T ratio per se.

scholarly journals Use of precision-fed cecectomized rooster assay and digestible indispensable amino acid scores to characterize plant- and yeast-concentrated proteins for inclusion in canine and feline diets1,2

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Lauren M Reilly ◽  
Patrick C von Schaumburg ◽  
Jolene M Hoke ◽  
Gary M Davenport ◽  
Pamela L Utterback ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Protein Quality ◽  
Essential Amino Acids ◽  
Protein Sources ◽  
Protein Concentrates ◽  
Indispensable Amino Acid ◽  
Limiting Amino Acid ◽  
Adult Cats ◽  
Novel Protein

Abstract Increased consumer interest in high-quality and novel protein sources has driven the demand for the inclusion of protein-rich ingredients in companion animal diets. Novel protein concentrates, with protein contents of at least 50%, have been used to satisfy these consumer demands. However, minimal information is available regarding the macronutrient composition and protein quality of these ingredients that is needed for proper formulation of pet foods. Therefore, the objectives of this study were to determine the macronutrient and amino acid compositions, standardized amino acid digestibility according to the precision-fed rooster assay, and protein quality using digestible indispensable amino acid score (DIAAS like) of pea protein (PP), potato protein (POP), faba bean protein (FBP), soy protein concentrate (SPC), and dried yeast (DY). Precision-fed rooster assays were conducted using cecectomized roosters to calculate standardized amino acid digestibility and true metabolizable energy corrected for nitrogen (TMEn). For all five protein concentrates, all essential amino acids were highly digestible (88.0% to 96.3%, dry matter basis) with differences (P < 0.05) in only lysine, methionine, and tryptophan digestibilities. The TMEn values were highest for POP (4.22 kcal/g) and DY (3.61 kcal/g). The DIAAS-like values for adult dogs indicated that methionine was the first-limiting amino acid in all protein concentrates except POP, where the first-limiting amino acid was tryptophan. Using Association of American Feed Control Officials (AAFCO)-recommended values for adult cats, DIAAS-like values for methionine were lowest (P < 0.05) for FBP at 81.5%, with all other amino acids for all protein concentrates over 100%. The National Research Council (NRC)-recommended allowances for adult cats indicated that DIAAS-like methionine values for PP (92.7%) and FBP (73.8%) were significantly lower (P < 0.05) with these being the first-limiting amino acids, with the remaining amino acids above 100% for the other protein concentrates. The protein quality and high essential amino acid digestibility of these protein concentrates indicate that they would be viable protein sources in canine and feline diets. However, additional complementary protein sources should be included to meet the requirements of all essential amino acids.

scholarly journals The effect of amino acid imbalance on nitrogen retention (biological value) in rats

1981 ◽  
Vol 45 (1) ◽  
pp. 175-181 ◽  
Author(s):  
B. O. Eggum ◽  
K. E. Bach Knudsen ◽  
Ingeborg Jacobsen
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Nitrogen Retention ◽  
Basal Diet ◽  
Single Amino Acid ◽  
Protein Utilization ◽  
Biological Value ◽  
Egg Protein ◽  
Amino Acid Imbalance ◽  
Barley Protein

1. Three series of nitrogen balance experiments were performed with growing rats to test the effect of amino acid imbalance on protein utilization. In Expt 1 egg protein was fed as a basal diet and supplemented with one amino acid at a time in the amounts originally found in egg protein. In Expt 2 the procedure was repeated with a barley diet, and in Expt 3 egg and potato proteins were fed together in various combinations.2. Doubling the single amino acids, especially arginine, in an egg-protein diet reduced biological value (BV) markedly. Also the branched-chain and the basic amino acids reduced BV significantly. In the barley-based diets negative effects were also observed when the concentration of the single amino acid was doubled. However, it was assumed that the observed deleterious effects of amino acid excess were partly due to an exaggerated lysine deficiency in the barley protein when non-limiting amino acids were added.3. The results with various combinations of egg and potato proteins showed that as potato protein was increased true protein digestibility decreased linearly, whereas BV decreased Curvilinearly. Maximum protein utilization was obtained with egg protein alone.

scholarly journals ASSESSMENT OF NUTRIENT POTENTIAL, MINERAL CONTENT AND AMINO ACID COMPOSITION OF Thaumatococcus daniellii LEAF PROTEIN CONCENTRATES

2014 ◽  
Vol 22 (22) ◽  
pp. 61-78
Author(s):  
A. SODAMADE
Keyword(s):  
Amino Acid ◽  
Mineral Content ◽  
Amino Acid Content ◽  
Amino Acid Profile ◽  
Proximate Analysis ◽  
Leaf Protein ◽  
Crude Fibre ◽  
Analytical Technique ◽  
Protein Concentrates ◽  
Nonessential Amino Acids

Freshly harvested Thaumatococcos daniellii, was plucked and processed for its vegetable leaf protein concentrates with a view to evaluate its proximate constituents, amino acid content and mineral composition. Proximate analysis was determined using standard analytical technique. The nutrient composition of the protein concentrates revealed; the moisture content (9.94±0.01), crude fat (6.69±0.23), crude fibre (13.06 ±_0.17), crude protein (52.07 ± 0.20), ash (15.10 ± 0.13) and Carbohydrate (1.12±0.43). The mineral content of the sample indicated that Ca, Mg, K, and Na are the most abundant minerals with the following values Na;70.6±0.42,Ca;19. 70±0.28, K; 90.3±0.42, Mg; 103.9±0. 76, other minerals that were presentin the sample in trace concentration are Fe(2.00 ± 0.46), Zn (2.90± 1.06), Mn (2.50± 0.12), Cu (0.2± 0.58), Pb (0.1± 0.44), while selenium were not detected in the sample indicating that the leaf concentrate is fit for dietary consumption. The amino acid profile reveals favourable nutritional balance with the presence of essential and nonessential amino acids except that tryptophan which was believed to be predominant in animal protein was not detected.

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