scholarly journals Protein and amino acid digestibility, biological value of protein and energy metabolizability of some leaf protein concentrates in chickens.

1984 ◽  
Vol 21 (2) ◽  
pp. 64-74
Author(s):  
Suwit TERAPUNTUWAT ◽  
Iwao TASAKI
Author(s):  
Ajay Iyer ◽  
Lisa Guerrier ◽  
Salomé Leveque ◽  
Charles S. Bestwick ◽  
Sylvia H. Duncan ◽  
...  

AbstractInvasive plants offer an interesting and unconventional source of protein and the considerable investment made towards their eradication can potentially be salvaged through their revalorisation. To identify viable sources, effective and high-throughput screening methods are required, as well as efficient procedures to isolate these components. Rigorous assessment of low-cost, high-throughput screening assays for total sugar, phenolics and protein was performed, and ninhydrin, Lever and Fast Blue assays were found to be most suitable owing to high reliability scores and false positive errors less than 1%. These assays were used to characterise invasive Scottish plants such as Gorse (Ulex europeans), Broom (Cystisus scoparius) and Fireweed (Chamaenerion angustifolium). Protein extraction (alkali-, heat- and enzyme assisted) were tested on these plants, and further purification (acid and ethanol precipitation, as well as ultrafiltration) procedures were tested on Gorse, based on protein recovery values. Cellulase treatment and ethanol precipitation gave the highest protein recovery (64.0 ± 0.5%) and purity (96.8 ± 0.1%) with Gorse. The amino acid profile of the purified protein revealed high levels of essential amino acids (34.8 ± 0.0%). Comparison of results with preceding literature revealed a strong association between amino acid profiles and overall protein recovery with the extraction method employed. The final purity of the protein concentrates was closely associated to the protein content of the initial plant mass. Leaf protein extraction technology can effectively raise crop harvest indices, revalorise underutilised plants and waste streams.


2021 ◽  
pp. 38-40
Author(s):  
Денис Сергеевич Куликов ◽  
Валентина Андреевна Гулакова ◽  
Валентина Васильевна Колпакова ◽  
Рузалия Владимировна Уланова

Из зерна нута получены белковые концентраты пищевого и кормового назначения с массовой долей белка на сухое вещество 83,22±0,35 % и 54,22±0,46 % соответственно и сбалансированным аминокислотным составом. Protein concentrates for food and feed purposes were obtained from chickpea grains with a mass fraction of protein per dry matter of 83.22±0.35 % and 54.22±0.46 %, respectively, and a balanced amino acid composition.


Author(s):  
A. SODAMADE

Freshly harvested Thaumatococcos daniellii, was plucked and processed for its vegetable leaf protein concentrates with a view to evaluate its proximate constituents, amino acid content and mineral composition. Proximate analysis was determined using standard analytical technique. The nutrient composition of the protein concentrates revealed; the moisture content (9.94±0.01), crude fat (6.69±0.23), crude fibre (13.06 ±_0.17), crude protein (52.07 ± 0.20), ash (15.10 ± 0.13) and Carbohydrate (1.12±0.43). The mineral content of the sample indicated that Ca, Mg, K, and Na are the most abundant minerals with the following values Na;70.6±0.42,Ca;19. 70±0.28, K; 90.3±0.42, Mg; 103.9±0. 76, other minerals that were presentin the sample in trace concentration are Fe(2.00 ± 0.46), Zn (2.90± 1.06), Mn (2.50± 0.12), Cu (0.2± 0.58), Pb (0.1± 0.44), while selenium were not detected in the sample indicating that the leaf concentrate is fit for dietary consumption. The amino acid profile reveals favourable nutritional balance with the presence of essential and nonessential amino acids except that tryptophan which was believed to be predominant in animal protein was not detected.


2020 ◽  
Vol 36 (4) ◽  
pp. 49-58
Author(s):  
V.V. Kolpakova ◽  
R.V. Ulanova ◽  
L.V. Chumikina ◽  
V.V. Bessonov

The goal of the study was to develop a biotechnological process for the production of protein concentrates via bioconversion of pea flour and whey, a secondary product of starch manufacture. Standard and special methods were used to analyze the chemical and biochemical composition of protein concentrates (amino acid, carbohydrate, and fractional) of flour, whey and protein concentrates. It was established that pea flour contains 52.28-57.05% water-soluble nitrogenous substances, 23.04-25.50% salt-soluble, 2.94-4.69% alcohol-soluble compounds, 0-0.61% of soluble glutenine, 6.67-10.40% alkali-soluble glutenine and 5.96-10.86% insoluble sclerotic substances. A mathematical model and optimal parameters of the enzymatic extraction of pea protein with a yield of 65-70% were developed. Ultrasonic exposure increased the yield of nitrogenous substances by 23.16 ± 0.69%, compared with the control without ultrasound. The protein concentrate had a mass fraction of nitrogenous substances of 72.48 ± 0.41% (Nx6.25) and a complete amino acid composition. The microbial conversion by the Saccharomyces cerevisiae 121 and Geotrichum candidum 977 cultures of starch whey which remained after protein precipitation allowed us to obtain feed concentrates from biomass and culture liquid with a protein mass fraction of 61.68-70.48% (Nx6.25). Protein concentrates positively affected the vital signs of rats and their excretory products. A technological scheme was developed to test the complex pea grain and starch whey processing under pilot conditions. pea, protein concentrate, extracts, whey, bioconversion, Geotrichum candidum, Saccharomyces cerevisiae, chemical composition, amino acid composition


1977 ◽  
Vol 42 (5) ◽  
pp. 1401-1401 ◽  
Author(s):  
G. ANELLI ◽  
R. FIORENTINI ◽  
L. MASSIGNAN ◽  
C. GALOPPINI

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