Protection by Tariff Barriers and International Transport Costs

The multimodal transportation development is a priority of Ukraine's transport policy due to the unique transport and geographical position at the intersection of European and Eurasian roads, international transport corridors.However, there is no legislation on the multimodal transportation in Ukraine. Thus, it is relevant to determine the legal and organizational basis of multimodal transportation, legal status of participants in such transportation, state support for multimodal transportation and related infrastructure. Today, the transport system of Ukraine has the low level of development of transport infrastructure to ensure the proper volume of the multimodal transportation. There are not enough multimodal transport terminals in Ukraine. Due to the lack of state support and the lack of a favorable investment climate, there is no development of existing multimodal infrastructure facilities. The formation procedure of tariffs for multimodal transportation in Ukraine is imperfect. The article considers the main provisions of the draft Law of Ukraine "On Multimodal Transportation" and reveals its imperfections in the formation of a single tariff. The international experience of rates formation and the main stages of this process are studied. It is offered the methodical approach of formation of the multimodal transportation`s single tariff based on unification of key elements of technological process on which transport costs are formed. The conceptual bases of ensuring the transport infrastructure development on the basis of multimodality have been improved. It is given the author's vision of the terms "multimodal transportation", "intermodal transportation", "combined transportation".

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Development of a Lyophilization Process for Campylobacter Bacteriophage Storage and Transport

Microorganisms ◽

10.3390/microorganisms8020282 ◽

2020 ◽

Vol 8 (2) ◽

pp. 282 ◽

Cited By ~ 2

Author(s):

Lu Liang ◽

Nicholas B. Carrigy ◽

Samuel Kariuki ◽

Peter Muturi ◽

Robert Onsare ◽

...

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Relative Humidity ◽

Pathogenic Bacteria ◽

Drying Process ◽

Transport Costs ◽

Food And Agriculture ◽

Transmission Electron ◽

Freeze Dried ◽

International Transport

Bacteriophages are a sustainable alternative to control pathogenic bacteria in the post-antibiotic era. Despite promising reports, there are still obstacles to phage use, notably titer stability and transport-associated expenses for applications in food and agriculture. In this study, we have developed a lyophilization approach to maintain phage titers, ensure efficacy and reduce transport costs of Campylobacter bacteriophages. Lyophilization methods were adopted with various excipients to enhance stabilization in combination with packaging options for international transport. Lyophilization of Eucampyvirinae CP30A using tryptone formed a cake that limited processing titer reduction to 0.35 ± 0.09 log10 PFU mL−1. Transmission electron microscopy revealed the initial titer reduction was associated with capsid collapse of a subpopulation. Freeze-dried phages were generally stable under refrigerated vacuum conditions and showed no significant titer changes over 3 months incubation at 4 °C (p = 0.29). Reduced stability was observed for lyophilized phages that were incubated either at 30 °C under vacuum or at 4 °C at 70% or 90% relative humidity. Refrigerated international transport and rehydration of the cake resulted in a total phage titer reduction of 0.81 ± 0.44 log10 PFU mL−1. A significantly higher titer loss was observed for phages that were not refrigerated during transport (2.03 ± 0.32 log10 PFU mL−1). We propose that lyophilization offers a convenient method to preserve and transport Campylobacter phages, with minimal titer reduction after the drying process.

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