Evaluation of the in Vitro Synergy of Polymyxin B-Based Combinations Against Polymyxin B -Resistant Gram-Negative Bacillus

2021 ◽  
Author(s):  
You Li ◽  
Siwei Guo ◽  
Xin Li ◽  
Yunsong Yu ◽  
Bingqian Yan ◽  
...  
Keyword(s):  
Polymyxin B ◽  
Gram Negative ◽  
Negative Bacillus ◽  
Gram Negative Bacillus

scholarly journals Effectiveness of polymyxin b immobilized fiber hemoperfusion in patients with septic shock due to Gram-negative bacillus infection: the PMXHP study

Critical Care ◽  
2015 ◽  
Vol 19 (Suppl 1) ◽  
pp. P131 ◽  
Author(s):  
N Saito ◽  
K Sugiyama ◽  
T Ohnuma ◽  
T Kanemura ◽  
M Nasu ◽  
...  
Keyword(s):  
Septic Shock ◽  
Polymyxin B ◽  
Gram Negative ◽  
Negative Bacillus ◽  
Gram Negative Bacillus

scholarly journals Efficacy of polymyxin B-immobilized fiber hemoperfusion for patients with septic shock caused by Gram-negative bacillus infection

PLoS ONE ◽  
2017 ◽  
Vol 12 (3) ◽  
pp. e0173633 ◽  
Author(s):  
Nobuyuki Saito ◽  
Kazuhiro Sugiyama ◽  
Testu Ohnuma ◽  
Takashi Kanemura ◽  
Michitaka Nasu ◽  
...  
Keyword(s):  
Septic Shock ◽  
Polymyxin B ◽  
Gram Negative ◽  
Negative Bacillus ◽  
Gram Negative Bacillus

scholarly journals Bipolar staining of gram-negative bacillus in cerebrospinal fluid

Blood ◽  
2014 ◽  
Vol 124 (18) ◽  
pp. 2892-2892
Author(s):  
Lori D. Racsa ◽  
Eileen M. Burd
Keyword(s):  
Cerebrospinal Fluid ◽  
Gram Negative ◽  
Negative Bacillus ◽  
Gram Negative Bacillus

scholarly journals Meningitis Caused by Salmonella Newport in a Five-Year-Old Child

2016 ◽  
Vol 2016 ◽  
pp. 1-4
Author(s):  
Ana De Malet ◽  
Sheila Ingerto ◽  
Israel Gañán
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Neurological Disorders ◽  
Invasive Disease ◽  
Salmonella Typhi ◽  
Gram Negative ◽  
Negative Bacillus ◽  
Salmonella Newport ◽  
Gram Negative Bacillus ◽  
The Central Nervous System

Salmonella Newport is a Gram-negative bacillus belonging to the Enterobacteria family and the nontyphi Salmonella (NTS), usually related to gastroenteritis. Main difference between NTS and Salmonella typhi is that the last one evolves to an invasive disease easier than NTS. These can progress to bacteremias in around 5% of cases and secondary focuses can appear occasionally, as in meningitis. An infection of the central nervous system is uncommon, considering its incidence in 0.6–8% of the cases; most of them are described in developing countries and mainly in childhood, especially neonates. Bacterial meningitis by NTS mostly affects immunosuppressed people in Europe. Prognosis is adverse, with a 50% mortality rate, mainly due to complications of infection: hydrocephalus, ventriculitis, abscesses, subdural empyema, or stroke. Choice antibiotic treatments are cefotaxime, ceftriaxone, or ceftazidime. The aim of this paper is to present a case of meningitis caused by Salmonella Newport diagnosed in a five-year-old girl living in a rural area of the province of Ourense (Spain), with favorable evolution and without neurological disorders.

scholarly journals Visualizing the Potential Impairment of Polymyxin B to Central Nervous System Through MR Susceptibility-Weighted Imaging

2021 ◽  
Vol 12 ◽  
Author(s):  
Ni Zhang ◽  
Lichong Zhu ◽  
Qiuhong Ouyang ◽  
Saisai Yue ◽  
Yichun Huang ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Polymyxin B ◽  
Susceptibility Weighted Imaging ◽  
Gram Negative Bacteria ◽  
Severe Toxicity ◽  
Gram Negative ◽  
The Impact

Polymyxin B (PMB) exert bactericidal effects on the cell wall of Gram-negative bacteria, leading to changes in the permeability of the cytoplasmic membrane and resulting in cell death, which is sensitive to the multi-resistant Gram-negative bacteria. However, the severe toxicity and adverse side effects largely hamper the clinical application of PMB. Although the molecular pathology of PMB neurotoxicity has been adequately studied at the cellular and molecular level. However, the impact of PMB on the physiological states of central nervous system in vivo may be quite different from that in vitro, which need to be further studied. Therefore, in the current study, the biocompatible ultra-uniform Fe3O4 nanoparticles were employed for noninvasively in vivo visualizing the potential impairment of PMB to the central nervous system. Systematic studies clearly reveal that the prepared Fe3O4 nanoparticles can serve as an appropriate magnetic resonance contrast agent with high transverse relaxivity and outstanding biosafety, which thus enables the following in vivo susceptibility-weighted imaging (SWI) studies on the PMB-treated mice models. As a result, it is first found that the blood-brain barrier (BBB) of mice may be impaired by successive PMB administration, displaying by the discrete punctate SWI signals distributed asymmetrically across brain regions in brain parenchyma. This result may pave a noninvasive approach for in-depth studies of PMB medication strategy, monitoring the BBB changes during PMB treatment, and even assessing the risk after PMB successive medication in multidrug-resistant Gram-negative bacterial infected patients from the perspective of medical imaging.

Melioidosis and glanders

2010 ◽  
pp. 768-772
Author(s):  
S.J. Peacock
Keyword(s):  
Diabetes Mellitus ◽  
Risk Factors ◽  
Northern Australia ◽  
Gram Negative ◽  
Negative Bacillus ◽  
North East ◽  
Serious Infection ◽  
The World ◽  
Gram Negative Bacillus ◽  
Sixth Decade

Melioidosis is a serious infection caused by the soil-dwelling Gram-negative bacillus Burkholderia pseudomallei. It is most commonly reported in north-east Thailand and northern Australia, but is increasingly recognized around the world. Infection is predominantly acquired through bacterial inoculation, often related to occupation, and mostly affects adults between the fourth and sixth decade who have risk factors such as diabetes mellitus and renal impairment....

scholarly journals Melioidosis with Septic Shock and Disseminated Infection in a Neutropenic Patient Receiving Chemotherapy

2020 ◽  
Vol 2020 ◽  
pp. 1-4
Author(s):  
Sitthi Sukauichai ◽  
Chantana Pattarowas
Keyword(s):  
Septic Shock ◽  
Mortality Rate ◽  
Burkholderia Pseudomallei ◽  
Neutropenic Patient ◽  
Pulmonary Nodules ◽  
Severe Infection ◽  
Gram Negative ◽  
Negative Bacillus ◽  
Gram Negative Bacillus ◽  
Chemotherapy Induced Neutropenia

Melioidosis is a bacterial infection, caused by Gram-negative bacillus, Burkholderia pseudomallei, widespread in Southeast Asia and the northern part of Australia, resulting in a high mortality rate in severe infection. However, it has rarely been reported in patients with chemotherapy-induced neutropenia. The authors described a case of melioidosis in a neutropenic patient presenting with septic shock after receiving chemotherapy. Blood and urine cultures were positive for Burkholderia pseudomallei, and CT scan showed multiple pulmonary nodules and hepatosplenic abscesses. The patient was successfully treated with antibiotics for the infection and with combined modalities for a malignancy.

A Gram-negative Bacillus Infection Causing Gill Lesions in Cultured Rockfish Sebastes schlegeli

2005 ◽  
Vol 40 (3) ◽  
pp. 143-145 ◽  
Author(s):  
Tatsuya Kobayashi ◽  
Makoto Imai ◽  
Yoshifumi Kawaguchi
Keyword(s):  
Sebastes Schlegeli ◽  
Gram Negative ◽  
Negative Bacillus ◽  
Gram Negative Bacillus

Polymyxin-B Stimulates Tumor Necrosis Factor-α Production by Human Peripheral Blood Mononuclear Cells

1998 ◽  
Vol 21 (5) ◽  
pp. 269-273 ◽  
Author(s):  
B.L. Jaber ◽  
S. Sundaram ◽  
M. Cendoroglo Neto ◽  
A.J. King ◽  
B.J.G. Pereira
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Tumor Necrosis ◽  
Mononuclear Cells ◽  
Cytokine Production ◽  
Polymyxin B ◽  
Peripheral Blood Mononuclear ◽  
Gram Negative ◽  
Blood Mononuclear Cells

Gram-negative bacterial lipopolysaccharide (LPS) is a well known stimulus for cytokine production, particularly interleukin-1 (IL-1) and tumor necrosis tactor alpha (TNFα). Polymyxin B (PMX-B) is a cationic polypeptide that binds to LPS, neutralizing its biological effects. PMX-B also disrupts gram-negative bacterial cell membrane phospholipids but is highly toxic to mammalian cells, therefore is of limited use. PMX-B is used as additive to media, as a way to handle LPS contamination. To derive benefit from the ability of PMX-B to neutralize lipid A in vivo while avoiding its systemic toxicity, PMX-B was covalently bound to polystyrene-derivative fibers, creating a hemoperfusion column (PMX-F) for the selective removal of circulating ET In vitro PMX-F hemoperfusion studies have demonstrated effective ET removal, using either the Limulus amebocyte lysate assay or TNFα production by peripheral blood mononuclear cells (PBMC) as an index of ET removal. However, the question whether PMX-B itself could stimulate human PBMC to produce cytokines has not been adequately addressed. We examined the effect of increasing concentrations of PMX-B on cytokine production by PBMC in vitro. PBMC harvested from healthy volunteers were incubated for 24 hours at 37°C with control (tissue culture media RPMI), or 5 µg/ml, 10 µg/ml, 20 µg/ml or 100 µg/ml PMX-B. At the end of 24 hours, PBMC were subjected to three freeze-thaw cycles, and total TNFα production (pg/2.5x106 PBMC) was measured by radioimmunoassay. Total TNFα production by PBMC was 163 ± 3 pg, 171 ± 9 pg, 164 ± 4 pg, 323 ± 63 pg and 331 ± 58 pg, in the control, PMX-B 5 µg/ml, 10 µg/ml, 20 µg/ml and 100 µg/ml conditions, respectively. Compared to controls (RPMI), the percentage increase in TNFα production by PBMC was 5 ± 6% (P=0.23), 1 ± 3% (P=0.45), 99 ± 40% (P=0.03) and 103 ± 36% (P=0.02) in the presence of 5 µg/ml, 10 µg/ml, 20 µg/ml and 100 µg/ml of PMX-B, respectively. Furthermore, total TNFα production correlated significantly with increasing concentrations of PMX-B (R=0.53, P=0.007). We conclude that the use of PMX-B in in vitro studies as an LPS-neutralizing agent, or in the experimental treatment of endotoxic or septic shock can lead to erroneous interpretations of cytokine production by PBMC, and should be used cautiously in in vitro systems at high concentrations.

Sign in / Sign up

Export Citation Format

Share Document