Cost-Effective High-Speed, Three-Dimensional Live-Cell Imaging of HIV-1 Transfer at the T Cell Virological Synapse

2021 ◽  
Author(s):  
Alice Sandmeyer ◽  
Lili Wang ◽  
Wolfgang Hübner ◽  
Marcel Müller ◽  
Benjamin Chen ◽  
...  
Keyword(s):  
T Cell ◽  
Live Cell Imaging ◽  
High Speed ◽  
Cell Imaging ◽  
Three Dimensional ◽  
Cost Effective ◽  
Live Cell ◽  
Virological Synapse ◽  
Hiv 1

scholarly journals High-speed synthetic aperture microscopy for live cell imaging

2011 ◽  
Vol 36 (2) ◽  
pp. 148 ◽  
Author(s):  
Moonseok Kim ◽  
Youngwoon Choi ◽  
Christopher Fang-Yen ◽  
Yongjin Sung ◽  
Ramachandra R. Dasari ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
High Speed ◽  
Cell Imaging ◽  
Live Cell ◽  
Synthetic Aperture ◽  
Synthetic Aperture Microscopy

scholarly journals CRISPR-mediated Multiplexed Live Cell Imaging of Nonrepetitive Genomic Loci

2020 ◽  
Author(s):  
Patricia A. Clow ◽  
Nathaniel Jillette ◽  
Jacqueline J. Zhu ◽  
Albert W. Cheng
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Repetitive Sequences ◽  
Binary Sequence ◽  
Three Dimensional ◽  
Live Cell ◽  
Live Cells ◽  
Imaging Method ◽  
Genomic Locus ◽  
Time Dynamics

AbstractThree-dimensional (3D) structures of the genome are dynamic, heterogeneous and functionally important. Live cell imaging has become the leading method for chromatin dynamics tracking. However, existing CRISPR- and TALE-based genomic labeling techniques have been hampered by laborious protocols and low signal-to-noise ratios (SNRs), and are thus mostly applicable to repetitive sequences. Here, we report a versatile CRISPR/Casilio-based imaging method, with an enhanced SNR, that allows for one nonrepetitive genomic locus to be labeled using a single sgRNA. We constructed Casilio dual-color probes to visualize the dynamic interactions of cohesin-bound elements in single live cells. By forming a binary sequence of multiple Casilio probes (PISCES) across a continuous stretch of DNA, we track the dynamic 3D folding of a 74kb genomic region over time. This method offers unprecedented resolution and scalability for delineating the dynamic 4D nucleome.One Sentence SummaryCasilio enables multiplexed live cell imaging of nonrepetitive DNA loci for illuminating the real-time dynamics of genome structures.

scholarly journals Easy and cost-effective stable positioning of suspension cells during live-cell imaging

2017 ◽  
Vol 4 (4) ◽  
pp. 80 ◽  
Author(s):  
Daniel Ivanusic ◽  
Kazimierz Madela ◽  
Joachim Denner
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Cost Effective ◽  
Live Cell ◽  
Suspension Cells

scholarly journals Live cell imaging of the HIV-1 life cycle

2008 ◽  
Vol 16 (12) ◽  
pp. 580-587 ◽  
Author(s):  
Edward M. Campbell ◽  
Thomas J. Hope
Keyword(s):  
Life Cycle ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Hiv 1

scholarly journals Advances in High-Speed Structured Illumination Microscopy

2021 ◽  
Vol 9 ◽  
Author(s):  
Tianyu Zhao ◽  
Zhaojun Wang ◽  
Tongsheng Chen ◽  
Ming Lei ◽  
Baoli Yao ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
High Speed ◽  
Cell Imaging ◽  
Super Resolution ◽  
Live Cell ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Light Power ◽  
Recent Developments ◽  
Super Resolution Microscopy

Super-resolution microscopy surpasses the diffraction limit to enable the observation of the fine details in sub-cellular structures and their dynamics in diverse biological processes within living cells. Structured illumination microscopy (SIM) uses a relatively low illumination light power compared with other super-resolution microscopies and has great potential to meet the demands of live-cell imaging. However, the imaging acquisition and reconstruction speeds limit its further applications. In this article, recent developments all targeted at improving the overall speed of SIM are reviewed. These comprise both hardware and software improvements, which include a reduction in the number of raw images, GPU acceleration, deep learning and the spatial domain reconstruction. We also discuss the application of these developments in live-cell imaging.

Three-Dimensional Unstained Live-Cell Imaging Using Stimulated Parametric Emission Microscopy

2009 ◽  
Vol 48 (9) ◽  
pp. 097003 ◽  
Author(s):  
Hieu M. Dang ◽  
Takehito Kawasumi ◽  
Gen Omura ◽  
Toshiyuki Umano ◽  
Shin'ichiro Kajiyama ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Three Dimensional ◽  
Live Cell ◽  
Emission Microscopy

scholarly journals Quantitative Live-Cell Imaging of Human Immunodeficiency Virus (HIV-1) Assembly

Viruses ◽  
2012 ◽  
Vol 4 (5) ◽  
pp. 777-799 ◽  
Author(s):  
Viola Baumgärtel ◽  
Barbara Müller ◽  
Don C. Lamb
Keyword(s):  
Human Immunodeficiency Virus ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Immunodeficiency Virus ◽  
Hiv 1
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