scholarly journals The Hybrid Drive: A Chronic Implant Device Combining Tetrode Arrays with Silicon Probes for Layer-Resolved Ensemble Electrophysiology in Freely Moving Mice

2021 ◽  
Author(s):  
Matteo Guardamagna ◽  
Ronny Eichler ◽  
Rafael Pedrosa ◽  
Arno Aarts ◽  
Arne Meyer ◽  
...  
Keyword(s):  
Hybrid Drive ◽  
Freely Moving ◽  
Silicon Probes

scholarly journals The Hybrid Drive: a chronic implant device combining tetrode arrays with silicon probes for layer-resolved ensemble electrophysiology in freely moving mice

2021 ◽  
Author(s):  
Matteo Guardamagna ◽  
Ronny Eichler ◽  
Rafael Pedrosa ◽  
Arno Aarts ◽  
Arne F Meyer ◽  
...  
Keyword(s):  
Oscillatory Activity ◽  
Hybrid Drive ◽  
Spatial And Temporal Scales ◽  
Large Numbers ◽  
Pyramidal Layer ◽  
Major Species ◽  
Weight Structure ◽  
Series Of Experiments ◽  
Electrophysiological Signal ◽  
Silicon Probes

Understanding the function of brain cortices requires simultaneous investigation at multiple spatial and temporal scales and to link neural activity to an animal's behavior. A major challenge is to measure within- and across-layer information in actively behaving animals, in particular in mice that have become a major species in neuroscience due to an extensive genetic toolkit. Here we describe the Hybrid Drive, a new chronic implant for mice that combines tetrode arrays to record within-layer information with silicon probes to simultaneously measure across-layer information. The flexible, open-source design allows custom spatial arrangements of tetrode arrays and silicon probes to generate areas-specific layouts. We show that large numbers of neurons and layer-resolved local field potentials can be recorded from the same brain region across weeks without loss in electrophysiological signal quality. The drive's light-weight structure (3.5 g) leaves animal behavior largely unchanged during a variety of experimental paradigms, enabling the study of rich, naturalistic behaviors. We demonstrate the power of the Hybrid Drive in a series of experiments linking the spiking activity of CA1 pyramidal layer neurons to the oscillatory activity across hippocampal layers.

scholarly journals Metal microdrive and head cap system for silicon probe recovery in freely moving rodent

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Mihály Vöröslakos ◽  
Peter C Petersen ◽  
Balázs Vöröslakos ◽  
György Buzsáki
Keyword(s):  
Temporal Dynamics ◽  
Brain Activity ◽  
Extracellular Recording ◽  
Gear System ◽  
High Yield ◽  
3D Printed ◽  
Effective Cost ◽  
Freely Moving ◽  
Procedural Instructions ◽  
Silicon Probes

High-yield electrophysiological extracellular recording in freely moving rodents provides a unique window into the temporal dynamics of neural circuits. Recording from unrestrained animals is critical to investigate brain activity during natural behaviors. The use and implantation of high-channel-count silicon probes represent the largest cost and experimental complexity associated with such recordings making a recoverable and reusable system desirable. To address this, we have designed and tested a novel 3D printed head-gear system for freely moving mice and rats. The system consists of a recoverable microdrive printed in stainless steel and a plastic head cap system, allowing researchers to reuse the silicon probes with ease, decreasing the effective cost, and the experimental effort and complexity. The cap designs are modular and provide structural protection and electrical shielding to the implanted hardware and electronics. We provide detailed procedural instructions allowing researchers to adapt and flexibly modify the head-gear system.

scholarly journals Metal microdrive and head cap system for silicon probe recovery in freely moving rodent

2020 ◽  
Author(s):  
Mihály Vöröslakos ◽  
Peter Petersen ◽  
Balázs Vöröslakos ◽  
György Buzsáki
Keyword(s):  
Temporal Dynamics ◽  
Brain Activity ◽  
Extracellular Recording ◽  
Gear System ◽  
High Yield ◽  
3D Printed ◽  
Effective Cost ◽  
Freely Moving ◽  
Procedural Instructions ◽  
Silicon Probes

High-yield electrophysiological extracellular recording in freely moving rodents provides a unique window into the temporal dynamics of neural circuits. Recording from unrestrained animals is critical to investigate brain activity during natural behaviors. The use and implantation of high-channel-count silicon probes represent the largest cost and experimental complexity associated with such recordings making a recoverable and reusable system desirable. To address this, we have designed and tested a novel 3D printed head-gear system for freely moving mice and rats. The system consists of a recoverable microdrive printed in stainless steel and a plastic head cap system, allowing researchers to reuse the silicon probes with ease, decreasing the effective cost, and the experimental effort and complexity. The cap designs are modular and provide structural protection and electrical shielding to the implanted hardware and electronics. We provide detailed procedural instructions allowing researchers to adapt and flexibly modify the head-gear system.

scholarly journals Long term recordings with immobile silicon probes in the mouse cortex

2015 ◽  
Author(s):  
Michael Okun ◽  
Matteo Carandini ◽  
Kenneth D. Harris
Keyword(s):  
Experimental Approach ◽  
Orientation Tuning ◽  
High Count ◽  
Reliable Technique ◽  
Chronic Recording ◽  
Mouse Cortex ◽  
Freely Moving ◽  
The Brain ◽  
Silicon Probes

AbstractA key experimental approach in neuroscience involves measuring neuronal activity in behaving animals with extracellular chronic recordings. Such chronic recordings were initially made with single electrodes and tetrodes, and are now increasingly performed with high-density, high-count silicon probes. A common way to achieve long-term chronic recording is to attach the probes to microdrives that progressively advance them into the brain and isolate them from mechanical forces. Here we report, however, that such microdrives are not strictly necessary. Indeed, we obtained high-quality recordings in both head-fixed and freely moving mice for several months following the implantation of immobile chronic probes. Probes implanted into the primary visual cortex yielded well-isolated single units whose spike waveform and orientation tuning were highly reproducible over time. Although electrode drift was not completely absent, at least 70% of neurons retained their waveform across days. Thus, immobile silicon probes represent a straightforward and reliable technique to obtain stable, long-term population recordings in mice, and to follow the activity of populations of well-isolated neurons over multiple days.

A Comparative study on secure routing algorithms SAODV and A-SAODV in Mobile AdHoc Networks (MANET) – The Enhancements of AODV

2012 ◽  
Vol 3 (2) ◽  
pp. 419-423
Author(s):  
JARUPULA RAJESHWAR ◽  
Dr G NARSIMHA
Keyword(s):  
Routing Protocol ◽  
Routing Protocols ◽  
Dynamic Network ◽  
Secure Routing ◽  
Mobile Adhoc Networks ◽  
Security Challenges ◽  
Basic Protocol ◽  
Freely Moving ◽  
Adhoc Networks ◽  
Secure Routing Protocols

A freely moving nodes forming as group to communicate among themselves are called as Mobile AdHoc Networks (MANET). Many applications are choosing this MANET for effective commutation due to its flexible nature in forming a network. But due to its openness characteristics it is posing many security challenges. As it has highly dynamic network topology security for routing is playing a major role. We have very good routing protocols for route discovery as well as for transporting data packers but most of them lack the feature of security like AODV. In this paper we are studying the basic protocol AODV and identify how it can be made secure. We are studying a protocol S-AODV which is a security extension of AODV which is called Secure AODV (S-AODV) and we are studying enhanced version of S-AODV routing protocol a Adaptive Secure AODV (A-SAODV). Finally we have described about the parameter to be taken for performance evaluation of different secure routing protocols

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