Cryopreservation of Network Bioactivity and Multi-Lineage Stromal Cell Differentiation Potential within Three-Dimensional Synthetic Hydrogels

2021 ◽  
Author(s):  
Sudhir Khetan
Keyword(s):  
Cell Differentiation ◽  
Stromal Cell ◽  
Three Dimensional ◽  
Differentiation Potential ◽  
Synthetic Hydrogels

scholarly journals DYRK1A Negatively Regulates CDK5-SOX2 Pathway and Self-Renewal of Glioblastoma Stem Cells

2021 ◽  
Vol 22 (8) ◽  
pp. 4011
Author(s):  
Brianna Chen ◽  
Dylan McCuaig-Walton ◽  
Sean Tan ◽  
Andrew P. Montgomery ◽  
Bryan W. Day ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Critical Role ◽  
Stem Cell Differentiation ◽  
Neural Progenitor Cell ◽  
Cellular Heterogeneity ◽  
Differentiation Potential ◽  
Glioblastoma Stem Cells ◽  
Glioblastoma Stem Cell

Glioblastoma display vast cellular heterogeneity, with glioblastoma stem cells (GSCs) at the apex. The critical role of GSCs in tumour growth and resistance to therapy highlights the need to delineate mechanisms that control stemness and differentiation potential of GSC. Dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A) regulates neural progenitor cell differentiation, but its role in cancer stem cell differentiation is largely unknown. Herein, we demonstrate that DYRK1A kinase is crucial for the differentiation commitment of glioblastoma stem cells. DYRK1A inhibition insulates the self-renewing population of GSCs from potent differentiation-inducing signals. Mechanistically, we show that DYRK1A promotes differentiation and limits stemness acquisition via deactivation of CDK5, an unconventional kinase recently described as an oncogene. DYRK1A-dependent inactivation of CDK5 results in decreased expression of the stemness gene SOX2 and promotes the commitment of GSC to differentiate. Our investigations of the novel DYRK1A-CDK5-SOX2 pathway provide further insights into the mechanisms underlying glioblastoma stem cell maintenance.

scholarly journals Effect of Uniaxial Tensile Cyclic Loading Regimes on Matrix Organization and Tenogenic Differentiation of Adipose-Derived Stem Cells Encapsulated within 3D Collagen Scaffolds

2017 ◽  
Vol 2017 ◽  
pp. 1-16 ◽  
Author(s):  
Gayathri Subramanian ◽  
Alexander Stasuk ◽  
Mostafa Elsaadany ◽  
Eda Yildirim-Ayan
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Expression Profiles ◽  
Three Dimensional ◽  
Uniaxial Tensile ◽  
Adipose Derived Stem Cells ◽  
Collagen Scaffolds ◽  
Differentiation Potential ◽  
Tenogenic Differentiation ◽  
Matrix Organization

Adipose-derived mesenchymal stem cells have become a popular cell choice for tendon repair strategies due to their relative abundance, ease of isolation, and ability to differentiate into tenocytes. In this study, we investigated the solo effect of different uniaxial tensile strains and loading frequencies on the matrix directionality and tenogenic differentiation of adipose-derived stem cells encapsulated within three-dimensional collagen scaffolds. Samples loaded at 0%, 2%, 4%, and 6% strains and 0.1 Hz and 1 Hz frequencies for 2 hours/day over a 7-day period using a custom-built uniaxial tensile strain bioreactor were characterized in terms of matrix organization, cell viability, and musculoskeletal gene expression profiles. The results displayed that the collagen fibers of the loaded samples exhibited increased matrix directionality with an increase in strain values. Gene expression analyses demonstrated that ASC-encapsulated collagen scaffolds loaded at 2% strain and 0.1 Hz frequency showed significant increases in extracellular matrix genes and tenogenic differentiation markers. Importantly, no cross-differentiation potential to osteogenic, chondrogenic, and myogenic lineages was observed at 2% strain and 0.1 Hz frequency loading condition. Thus, 2% strain and 0.1 Hz frequency were identified as the appropriate mechanical loading regime to induce tenogenic differentiation of adipose-derived stem cells cultured in a three-dimensional environment.

The support of bone marrow stromal cell differentiation by airbrushed nanofiber scaffolds

Biomaterials ◽  
2013 ◽  
Vol 34 (10) ◽  
pp. 2389-2398 ◽  
Author(s):  
Wojtek Tutak ◽  
Sumona Sarkar ◽  
Sheng Lin-Gibson ◽  
Tanya M. Farooque ◽  
Giri Jyotsnendu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Differentiation ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Marrow Stromal Cell ◽  
Nanofiber Scaffolds

Microbioreactors for Raman Microscopy of Stromal Cell Differentiation

2010 ◽  
Vol 82 (5) ◽  
pp. 1844-1850 ◽  
Author(s):  
Vishnu Vardhan Pully ◽  
Aufried Lenferink ◽  
Henk-Jan van Manen ◽  
Vinod Subramaniam ◽  
Clemens A. van Blitterswijk ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Stromal Cell ◽  
Raman Microscopy

Sex Steroids Regulate Epithelial–Stromal Cell Cross Talk and Trophoblast Attachment Invasion in a Three-Dimensional Human Endometrial Culture System

2013 ◽  
Vol 19 (9) ◽  
pp. 676-687 ◽  
Author(s):  
Hai Wang ◽  
Silvina Bocca ◽  
Sandra Anderson ◽  
Liang Yu ◽  
Bhaskara S. Rhavi ◽  
...  
Keyword(s):  
Cross Talk ◽  
Sex Steroids ◽  
Culture System ◽  
Stromal Cell ◽  
Three Dimensional

scholarly journals Tensile Loading Modulates Bone Marrow Stromal Cell Differentiation and the Development of Engineered Fibrocartilage Constructs

2010 ◽  
Vol 16 (6) ◽  
pp. 1913-1923 ◽  
Author(s):  
John T. Connelly ◽  
Eric J. Vanderploeg ◽  
Janna K. Mouw ◽  
Christopher G. Wilson ◽  
Marc E. Levenston
Keyword(s):  
Bone Marrow ◽  
Cell Differentiation ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Tensile Loading ◽  
Marrow Stromal Cell
Sign in / Sign up

Export Citation Format

Share Document