Sex Steroids Regulate Epithelial–Stromal Cell Cross Talk and Trophoblast Attachment Invasion in a Three-Dimensional Human Endometrial Culture System

2013 ◽  
Vol 19 (9) ◽  
pp. 676-687 ◽  
Author(s):  
Hai Wang ◽  
Silvina Bocca ◽  
Sandra Anderson ◽  
Liang Yu ◽  
Bhaskara S. Rhavi ◽  
...  
Keyword(s):  
Cross Talk ◽  
Sex Steroids ◽  
Culture System ◽  
Stromal Cell ◽  
Three Dimensional

scholarly journals Sex steroids influence organizational but not functional decidualization of feline endometrial cells in a 3D culture system†

2019 ◽  
Vol 101 (5) ◽  
pp. 906-915 ◽  
Author(s):  
Kathryn Wilsterman ◽  
Xinmiao Bao ◽  
Allegra D Estrada ◽  
Pierre Comizzoli ◽  
George E Bentley
Keyword(s):  
Sex Steroids ◽  
Culture System ◽  
Three Dimensional ◽  
3D Culture ◽  
In Vitro Models ◽  
Sex Steroid ◽  
Endometrial Cells ◽  
Organizational Patterns

Abstract Successful implantation requires complex signaling between the uterine endometrium and the blastocyst. Prior to the blastocyst reaching the uterus, the endometrium is remodeled by sex steroids and other signals to render the endometrium receptive. In vitro models have facilitated major advances in our understanding of endometrium preparation and endometrial–blastocyst communication in mice and humans, but these systems have not been widely adapted for use in other models which might generate a deeper understanding of these processes. The objective of our study was to use a recently developed, three-dimensional culture system to identify specific roles of female sex steroids in remodeling the organization and function of feline endometrial cells. We treated endometrial cells with physiologically relevant concentrations of estradiol and progesterone, either in isolation or in combination, for 1 week. We then examined size and density of three-dimensional structures, and quantified expression of candidate genes known to vary in response to sex steroid treatments and that have functional relevance to the decidualization process. Combined sex steroid treatments recapitulated organizational patterns seen in vivo; however, sex steroid manipulations did not induce expected changes to expression of decidualization-related genes. Our results demonstrate that sex steroids may not be sufficient for complete decidualization and preparation of the feline endometrium, thereby highlighting key areas of opportunity for further study and suggesting some unique functions of felid uterine tissues.

scholarly journals Isolation and culture of human endometrial cells in a three-dimensional culture system

Reproduction ◽  
1994 ◽  
Vol 101 (2) ◽  
pp. 327-332 ◽  
Author(s):  
U. Bentin-Ley ◽  
B. Pedersen ◽  
S. Lindenberg ◽  
J. F. Larsen ◽  
L. Hamberger ◽  
...  
Keyword(s):  
Culture System ◽  
Three Dimensional ◽  
Endometrial Cells ◽  
Three Dimensional Culture

scholarly journals Loss of Nitric Oxide Induces Fibrogenic Response in Organotypic 3D Co-Culture of Mammary Epithelia and Fibroblasts—An Indicator for Breast Carcinogenesis

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2815
Author(s):  
Gang Ren ◽  
Xunzhen Zheng ◽  
Vandana Sharma ◽  
Joshua Letson ◽  
Andrea L. Nestor-Kalinoski ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Culture System ◽  
Epithelial Mesenchymal Transition ◽  
Precancerous Lesions ◽  
Three Dimensional ◽  
Epidemiological Studies ◽  
Mesenchymal Transition ◽  
In Vitro Response ◽  
Mammary Epithelia

Excessive myofibroblast activation, which leads to dysregulated collagen deposition and the stiffening of the extracellular matrix (ECM), plays pivotal roles in cancer initiation and progression. Cumulative evidence attests to the cancer-causing effects of a number of fibrogenic factors found in the environment, diseases and drugs. While identifying such factors largely depends on epidemiological studies, it would be of great importance to develop a robust in vitro method to demonstrate the causal relationship between fibrosis and cancer. Here, we tested whether our recently developed organotypic three-dimensional (3D) co-culture would be suitable for that purpose. This co-culture system utilizes the discontinuous ECM to separately culture mammary epithelia and fibroblasts in the discrete matrices to model the complexity of the mammary gland. We observed that pharmaceutical deprivation of nitric oxide (NO) in 3D co-cultures induced myofibroblast differentiation of the stroma as well as the occurrence of epithelial–mesenchymal transition (EMT) of the parenchyma. Such in vitro response to NO deprivation was unique to co-cultures and closely mimicked the phenotype of NO-depleted mammary glands exhibiting stromal desmoplasia and precancerous lesions undergoing EMT. These results suggest that this novel 3D co-culture system could be utilized in the deep mechanistic studies of the linkage between fibrosis and cancer.

Characterization of Adult Stem/Progenitor Cell Populations from Bone Marrow in a Three-Dimensional Collagen Gel Culture System

2012 ◽  
Vol 21 (9) ◽  
pp. 2021-2032 ◽  
Author(s):  
Silvia Claros ◽  
Noela Rodríguez-Losada ◽  
Encarnación Cruz ◽  
Enrique Guerado ◽  
José Becerra ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Culture System ◽  
Progenitor Cell ◽  
Three Dimensional ◽  
Collagen Gel ◽  
Cell Populations ◽  
Collagen Gel Culture ◽  
Gel Culture

Novel three-dimensional long-term bone marrow culture system using polymer particles with grafted epoxy-polymer-chains supports the proliferation and differentiation of hematopoietic stem cells

2011 ◽  
Vol 236 (11) ◽  
pp. 1342-1350 ◽  
Author(s):  
Yukio Hirabayashi ◽  
Yoshihiro Hatta ◽  
Jin Takeuchi ◽  
Isao Tsuboi ◽  
Tomonori Harada ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Culture System ◽  
3D Structure ◽  
Three Dimensional ◽  
Hematopoietic Cells ◽  
3D Culture ◽  
Polymer Chains ◽  
Polymer Particles ◽  
Hematopoietic Stem ◽  
3D Culture System

Hematopoiesis occurs in the bone marrow, where primitive hematopoietic cells proliferate and differentiate in close association with a three-dimensional (3D) hematopoietic microenvironment composed of stromal cells. We examined the hematopoietic supportive ability of stromal cells in a 3D culture system using polymer particles with grafted epoxy polymer chains. Umbilical cord blood-derived CD34+ cells were co-cultivated with MS-5 stromal cells. They formed a 3D structure in the culture dish in the presence of particles, and the total numbers of cells and the numbers of hematopoietic progenitor cells, including colony-forming unit (CFU)-Mix, CFU-granulocyte-macrophage, CFU-megakaryocyte and burst-forming unit-erythroid, were measured every seven days. The hematopoietic supportive activity of the 3D culture containing polymer particles and stromal cells was superior to that of 2D culture, and allowed the expansion and maintenance of hematopoietic progenitor cells for more than 12 weeks. Various types of hematopoietic cells, including granulocytes, macrophages and megakaryocytes at different maturation stages, appeared in the 3D culture, suggesting that the CD34+ cells were able to differentiate into a range of blood cell types. Morphological examination showed that MS-5 stromal cells grew on the surface of the particles and bridged the gaps between them to form a 3D structure. Hematopoietic cells slipped into the 3D layer and proliferated within it, relying on the presence of the MS-5 cells. These results suggest that this 3D culture system using polymer particles reproduced the hematopoietic phenomenon in vitro, and might thus provide a new tool for investigating hematopoietic stem cell–stromal cell interactions.

System cross-talk and three-dimensional cue issues in autostereoscopic displays

2013 ◽  
Vol 22 (1) ◽  
pp. 013032 ◽  
Author(s):  
Pei-Chia Wang ◽  
Sheue-Ling Hwang ◽  
Hsin-Ying Huang ◽  
Chih-Fei Chuang
Keyword(s):  
Cross Talk ◽  
Three Dimensional ◽  
Autostereoscopic Displays

Ho:YAG laser: intervertebral disk cell interaction using three-dimensional cell culture system

2000 ◽  
Author(s):  
Masato Sato ◽  
Miya Ishihara ◽  
Tsunenori Arai ◽  
Takashi Asazuma ◽  
Toshiyuki Kikuchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Three Dimensional ◽  
Cell Interaction ◽  
Intervertebral Disk ◽  
Cell Culture System ◽  
Ho:Yag Laser ◽  
Dimensional Cell

scholarly journals Coculture of Spermatogonia With Somatic Cells in a Novel Three-Dimensional Soft-Agar-Culture-System

2008 ◽  
Vol 29 (3) ◽  
pp. 312-329 ◽  
Author(s):  
J.-B. Stukenborg ◽  
J. Wistuba ◽  
C. M. Luetjens ◽  
M. A. Elhija ◽  
M. Huleihel ◽  
...  
Keyword(s):  
Culture System ◽  
Somatic Cells ◽  
Three Dimensional ◽  
Soft Agar ◽  
Agar Culture
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