Mesenchymal Stem Cells-Derived Exosomal H19 Promotes Trophoblast Cell Invasion and Migration by Down-Regulating Let-7b in Preeclampsia

2019 ◽  
Author(s):  
Yang Chen ◽  
Haiyan Ding ◽  
Min Wei ◽  
Wenhui Zha ◽  
Shuang Guan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Invasion ◽  
Trophoblast Cell ◽  
Invasion And Migration ◽  
And Migration

scholarly journals MSC-Secreted Exosomal H19 Promotes Trophoblast Cell Invasion and Migration by Downregulating let-7b and Upregulating FOXO1

2020 ◽  
Vol 19 ◽  
pp. 1237-1249
Author(s):  
Yang Chen ◽  
Haiyan Ding ◽  
Min Wei ◽  
Wenhui Zha ◽  
Shuang Guan ◽  
...  
Keyword(s):  
Cell Invasion ◽  
Trophoblast Cell ◽  
Invasion And Migration ◽  
And Migration

Bone Marrow Mesenchymal Stem Cells (BMSC)-Derived MicroRNA-189 Inhibits Glioma Tumorigenesis Through Suppressing Tumor Necrosis Factor-α (TNF-α)-Mediated Nuclear Factor Kappa Light Chain Enhancer of Activated B Cells (NF-κB) Signaling Pathway

2022 ◽  
Vol 12 (3) ◽  
pp. 581-587
Author(s):  
Wenxu Rao ◽  
Kang Yin
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Signaling Pathway ◽  
Inflammatory Factors ◽  
Invasion And Migration ◽  
Marrow Mesenchymal Stem Cells ◽  
And Migration

This study aims at investigating the mechanism underlying bone marrow mesenchymal stem cells (BMSC) function in glioma. Glioma cells were administered with plasmids loading NF-κB siRNA, microRNA (miRNA)-189 inhibitor, or miR-189 mimics for transfection followed by analysis of miR-189 expression by RT-qPCR, cell apoptosis by flow cytometry, cell proliferation by MTT assay,invasion and migration by Transwell assay, inflammatory factors secretion by ELISA as well as proteins expression by western blot. A mouse model of glioma was established to detect the in vivo effect of BMSCs. miR-189 was lowly expressed in glioma cell lines but enriched in BMSCs. When miR-189 was silenced, cell proliferation, invasion and migration were potentiated and apoptosis was decreased, along with enhancement of N-cadherin, Vimentin, MMP-2 and and MMP-9, and decline in Bax, cleaved casepase-3 and cleaved PARP. Silencing of NF-κB reversed the effect of miR-189 inhibitor on cell progression, accompanied with reduction of inflammatory factors. BMSCs treatment effectively promoted miR-189 expression in glioma and inactivated TNF-α/NF-κB signaling, thereby suppressing tumor growth. In conclusion, miR-189 derived from BMSC inhibits glioma progression through regulation of TNF-α/NF-κB signaling pathway.

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