Curcumin-Activated Mesenchymal Stem Cells Derived from Human Umbilical Cord and Their Effects on MPTP-Mice Model of Parkinson's Disease: A New Biological Therapy for Parkinson's Disease

2019 ◽  
Author(s):  
Yun-Liang Wang ◽  
Xin-Shan Liu ◽  
Shan-Shan Wang ◽  
Peng Xue ◽  
Zhi-Lei Zeng ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Stem Cells ◽  
Parkinson's Disease ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Biological Therapy ◽  
Human Umbilical Cord ◽  
Mice Model

scholarly journals Human Umbilical Cord Mesenchymal Stem Cells Infected with Adenovirus ExpressingHGFPromote Regeneration of Damaged Neuron Cells in a Parkinson’s Disease Model

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Xin-Shan Liu ◽  
Jin-Feng Li ◽  
Shan-Shan Wang ◽  
Yu-Tong Wang ◽  
Yu-Zhen Zhang ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Stem Cells ◽  
Parkinson's Disease ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Calcium Binding ◽  
Cell Model ◽  
Disease Model ◽  
Neuroblastoma Cells ◽  
Human Umbilical Cord

Parkinson’s disease (PD) is a neurodegenerative movement disorder that is characterized by the progressive degeneration of the dopaminergic (DA) pathway. Mesenchymal stem cells derived from human umbilical cord (hUC-MSCs) have great potential for developing a therapeutic agent as such. HGF is a multifunctional mediator originally identified in hepatocytes and has recently been reported to possess various neuroprotective properties. This study was designed to investigate the protective effect of hUC-MSCs infected by an adenovirus carrying theHGFgene on the PD cell model induced by MPP+ on human bone marrow neuroblastoma cells. Our results provide evidence that the cultural supernatant from hUC-MSCs expressing HGF could promote regeneration of damaged PD cells at higher efficacy than the supernatant from hUC-MSCs alone. And intracellular free Ca2+obviously decreased after treatment with cultural supernatant from hUC-MSCs expressing HGF, while the expression of CaBP-D28k, an intracellular calcium binding protein, increased. Therefore our study clearly demonstrated that cultural supernatant of MSC overexpressingHGFwas capable of eliciting regeneration of damaged PD model cells. This effect was probably achieved through the regulation of intracellular Ca2+levels by modulating of CaBP-D28k expression.

scholarly journals Therapeutic Effects of CUR-Activated Human Umbilical Cord Mesenchymal Stem Cells on 1-Methyl-4-phenylpyridine-Induced Parkinson’s Disease Cell Model

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Li Jinfeng ◽  
Wang Yunliang ◽  
Liu Xinshan ◽  
Wang Yutong ◽  
Wang Shanshan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Parkinson’S Disease ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Pc12 Cells ◽  
Umbilical Cord ◽  
Cell Model ◽  
Human Umbilical Cord ◽  
Therapeutic Effects ◽  
Proliferation And Differentiation

The purpose of this study is to evaluate the therapeutic effects of human umbilical cord-derived mesenchymal stem cells (hUC-MSC) activated by curcumin (CUR) on PC12 cells induced by 1-methyl-4-phenylpyridinium ion (MPP+), a cell model of Parkinson’s disease (PD). The supernatant of hUC-MSC and hUC-MSC activated by 5 µmol/L CUR (hUC-MSC-CUR) were collected in accordance with the same concentration. The cell proliferation and differentiation potential to dopaminergic neuronal cells and antioxidation were observed in PC12 cells after being treated with the above two supernatants and 5 µmol/L CUR. The results showed that the hUC-MSC-CUR could more obviously promote the proliferation and the expression of tyrosine hydroxylase (TH) and microtubule associated protein-2 (MAP2) and significantly decreased the expression of nitric oxide (NO) and inducible nitric oxide synthase (iNOS) in PC12 cells. Furtherly, cytokines detection gave a clue that the expression of IL-6, IL-10, and NGF was significantly higher in the group treated with the hUC-MSC-CUR compared to those of other two groups. Therefore, the hUC-MSC-CUR may be a potential strategy to promote the proliferation and differentiation of PD cell model, therefore providing new insights into a novel therapeutic approach in PD.

scholarly journals Human Umbilical Cord Matrix Stem Cells: Preliminary Characterization and Effect of Transplantation in a Rodent Model of Parkinson's Disease

Stem Cells ◽  
2006 ◽  
Vol 24 (3) ◽  
pp. 781-792 ◽  
Author(s):  
Mark L. Weiss ◽  
Satish Medicetty ◽  
Amber R. Bledsoe ◽  
Raja Shekar Rachakatla ◽  
Michael Choi ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Stem Cells ◽  
Parkinson's Disease ◽  
Umbilical Cord ◽  
Rodent Model ◽  
Human Umbilical Cord ◽  
Preliminary Characterization ◽  
Umbilical Cord Matrix

scholarly journals The Effect of MSCs Derived from the Human Umbilical Cord Transduced by Fibroblast Growth Factor-20 on Parkinson’s Disease

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Li Jinfeng ◽  
Wang Yunliang ◽  
Liu Xinshan ◽  
Wang Shanshan ◽  
Xu Chunyang ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Growth Factor ◽  
Cell Therapy ◽  
Fibroblast Growth Factor ◽  
Umbilical Cord ◽  
Human Umbilical Cord ◽  
Fibroblast Growth ◽  
Mice Model ◽  
Factor 20

Cell therapy is a potential therapeutic approach for Parkinson’s disease (PD). Mesenchymal stem cells derived from the human umbilical cord (hUC-MSCs) give priority to PD patients because of multiple advantages. The appropriate gene transduction of hUC-MSC before transplantation is a promising procedure for cell therapy. Fibroblast growth factor-20 (FGF-20) has been shown to protect dopaminergic neurons against a range of toxic insults in vitro. In this study, the hUC-MSCs were gene transduced with FGF-20, and then we transplanted them into the PD mice model. The results showed that MSC-FGF-20 treatment obviously improved the behavior of PD, accompanied by the increase of tyrosine carboxylase- (TH-) positive cell and dopamine (DA). Furtherly, immunohistochemistry disclosed that MSC-FGF-20 obviously promoted the degradation of nuclear factor-κB (NF-κB), a transcription factor that controls genes encoding proinflammatory cytokines, highly expressed in the nigrostriatal dopaminergic regions in PD patients. Therefore, MSC-FGF-20 has a potential for improving PD, closely related to the degradation of NF-κB.

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