Genome Introgression of Festuca mairei into Lolium perenne Detected by SSR and RAPD Markers

Crop Science ◽  
2003 ◽  
Vol 43 (6) ◽  
pp. 2154-2161 ◽  
Author(s):  
J. P. Wang ◽  
S. S. Bughrara ◽  
D. A. Sleper
Keyword(s):  
Lolium Perenne ◽  
Rapd Markers ◽  
Festuca Mairei

Genomic in situ hybridization (GISH) reveals high chromosome pairing affinity between Lolium perenne and Festuca mairei

Genome ◽  
2000 ◽  
Vol 43 (2) ◽  
pp. 398-403 ◽  
Author(s):  
Mingshu Cao ◽  
David A Sleper ◽  
Fenggao Dong ◽  
Jiming Jiang
Keyword(s):  
In Situ Hybridization ◽  
Lolium Perenne ◽  
Chromosome Pairing ◽  
Cytogenetic Analysis ◽  
Direct Evidence ◽  
Genomic In Situ Hybridization ◽  
Genomic Constitution ◽  
Conventional Cytogenetic Analysis ◽  
Festuca Mairei

Intergeneric hybridizations have been made between species of Lolium and Festuca. It has been demonstrated, largely through conventional cytogenetic analysis, that the genomes of the two genera are related, however, much information is lacking on exactly how closely related the genomes are between the two species. We applied genomic in situ hybridization (GISH) techniques to the F1 hybrids of tetraploid Festuca mairei with a genomic constitution of M1M1M2M2 and diploid Lolium perenne with a genomic constitution of LL. It was shown in the triploid hybrids (LM1M2) that the chromosomes of M1 and M2 from F. mairei could pair with each other, and it was further discovered that L chromosomes of L. perenne paired with M1 and M2 chromosomes. Our results showed that meiocytes of Lolium-Festuca are amenable to GISH analysis, and provided direct evidence for the hypothesis that the chromosomes of Lolium and Festuca may be genetically equivalent and that reciprocal mixing of the genomes may be possible. Key words: Lolium, Festuca, in situ hybridization, meiosis.

scholarly journals Random Amplified Polymorphic DNA in Perennial Ryegrass: A Comparison of Bulk Samples vs. Individuals

HortScience ◽  
1994 ◽  
Vol 29 (6) ◽  
pp. 624-626 ◽  
Author(s):  
Patricia M. Sweeney ◽  
T. Karl Danneberger
Keyword(s):  
Lolium Perenne ◽  
Perennial Ryegrass ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Heterogeneous Population ◽  
Arbitrary Primers ◽  
Synthetic Cultivars

The usefulness of random amplified polymorphic DNA (RAPD) in characterizing two perennial ryegrass (Lolium perenne L.) synthetic cultivars, `Accolade' and `Caravelle', was tested. Two out of 10 arbitrary primers produced three RAPD markers that distinguished bulk samples of 30 seedlings. Additional fragments were apparent when DNA from individual seedlings was amplified. Amplification products from bulk samples were not simply the sum of amplification products of individual seedlings and did not reflect all the diversity within or between the cultivars. The study illustrates the need to screen individuals to accurately evaluate the genotypic composition of a synthetic cultivar or heterogeneous population.

Cytogenetic Analysis of Festuca Species and Amphiploids between Festuca mairei and Lolium perenne

Crop Science ◽  
2003 ◽  
Vol 43 (5) ◽  
pp. 1659-1662 ◽  
Author(s):  
Mingshu Cao ◽  
Suleiman S. Bughrara ◽  
David A. Sleper
Keyword(s):  
Lolium Perenne ◽  
Cytogenetic Analysis ◽  
Festuca Mairei

Parental genome composition and genetic classifications of derivatives from intergeneric crosses of Festuca mairei and Lolium perenne

2008 ◽  
Vol 23 (2) ◽  
pp. 299-309 ◽  
Author(s):  
Jianping P. Wang ◽  
Suleiman S. Bughrara ◽  
Rouf M. A. Mian ◽  
Malay C. Saha ◽  
David A. Sleper
Keyword(s):  
Lolium Perenne ◽  
Parental Genome ◽  
Genome Composition ◽  
Intergeneric Crosses ◽  
Festuca Mairei

Techniques for preparing the Lolium perenne coleorhiza for scanning electron microscope evaluation

1992 ◽  
Vol 50 (1) ◽  
pp. 766-767
Author(s):  
Susan B.G. Debaene ◽  
John S. Gardner ◽  
Phil S. Allen
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Lolium Perenne ◽  
Morphological Study ◽  
Root Hairs ◽  
Inner Pressure ◽  
Water Potentials ◽  
Radicle Emergence ◽  
Standard Fixation ◽  
Scanning Electron

The coleorhiza is a nonvascular sheath that encloses the embryonic radicle in Poaceae, and is generally the first tissue to emerge during germination. Delicate hairlike extensions develop from some coleorhiza cells prior to radicle emergence. Similar to root hairs, coleorhiza hairs are extremely sensitive to desiccation and are damaged by exposure to negative water potentials. The coleorhiza of Lolium perenne is somewhat spherical when first visible, after which a knob forms at a right angle to the caryopsis due to inner pressure from the elongating radicle. This knob increases in length until the radicle finally punctures the coleorhiza. Standard fixation procedures cause severe desiccation of coleorhiza cells and hairs, making morphological study of the coleorhiza difficult. This study was conducted to determine a more successful process for coleorhiza preservation.

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