Use of genome-specific repetitive DNA sequences to monitor chromatin introgression from Festuca mairei into Lolium perenne

2001 ◽  
Vol 103 (2-3) ◽  
pp. 248-253 ◽  
Author(s):  
M. Cao ◽  
D. A. Sleper
Keyword(s):  
Lolium Perenne ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Repetitive Dna Sequences ◽  
Festuca Mairei

scholarly journals Repetitive DNA sequences near three humanβ-type globin genes

1980 ◽  
Vol 8 (15) ◽  
pp. 3319-3333 ◽  
Author(s):  
Lesley W. Coggins ◽  
G.Joan Grindlay ◽  
J.Keith Vass ◽  
Alison A. Slater ◽  
Paul Montague ◽  
...  
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
Repetitive Dna Sequences ◽  
Globin Genes

Fluorescence in situ hybridization on plant extended chromatin DNA fibers for single-copy and repetitive DNA sequences

2011 ◽  
Vol 30 (9) ◽  
pp. 1779-1786 ◽  
Author(s):  
Kun Yang ◽  
Hecui Zhang ◽  
Richard Converse ◽  
Yong Wang ◽  
Xiaoying Rong ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Repetitive Dna ◽  
Dna Sequences ◽  
Single Copy ◽  
Repetitive Dna Sequences

Wheat specific repetitive DNA sequences ? construction and characterization of four different genomic clones

1986 ◽  
Vol 72 (2) ◽  
pp. 207-210 ◽  
Author(s):  
M. Metzlaff ◽  
W. Troebner ◽  
F. Baldauf ◽  
R. Schlegel ◽  
J. Cullum
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
Repetitive Dna Sequences ◽  
Genomic Clones

scholarly journals Repetitive DNA sequences accelerate molecular cytogenetic research in plants with small chromosomes

2019 ◽  
Vol 24 (2) ◽  
pp. 82
Author(s):  
Agus Budi Setiawan ◽  
Ari Wibowo ◽  
Chee How Teo ◽  
Shinji Kikuchi ◽  
Takato Koba
Keyword(s):  
Repetitive Dna ◽  
Dna Sequences ◽  
5S Rdna ◽  
Repetitive Dna Sequences ◽  
Type I ◽  
Chromosome Identification ◽  
Metaphase Chromosomes ◽  
Homologous Chromosomes ◽  
Centromeric Sequence ◽  
Subtelomeric Sequence

Repetitive DNA sequences are highly abundant in plant genomes and are favorable probes for chromosome identification in plants. However, it is difficult to conduct studies on the details of metaphase chromosome structures in plants with small chromosomes due to their highly condensed status. Therefore, identification of homologous chromosomes for karyotyping and analyzing chromosome structures is a challenging issue for cytogeneticists without specific probes and precise chromosome stages. In this study, five repetitive DNA probes, i.e., 5S and 45S ribosomal DNAs (rDNAs), melon centromeric sequence (Cmcent), cucumber subtelomeric sequence (Type I), and microsatellite (CT)10 repeats, were used to identify primary constrictions and homologous chromosomes for karyotyping. Four and two loci of 45S rDNA were respectively observed on metaphase and pachytene chromosomes of Abelia × grandiflora. Cmcent was detected on both primary constrictions of melon pachytene and metaphase chromosomes. Furthermore, one pair of 5S rDNA signals were hybridized on melon metaphase chromosomes. Eight and two loci of 45S and 5S rDNA were respectively detected on cucumber chromosomes. Type I and (CT)10 probes were specifically hybridized on subtelomeric and interstitial regions on the chromosomes, respectively. These results suggest that repetitive DNA sequences are versatile probes for chromosome identification in plants with small chromosomes, particularly for karyotyping analyses.

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