Screening the Watermelon Germplasm Collection for Resistance to Papaya Ringspot Virus Type‐W

Crop Science ◽  
2002 ◽  
Vol 42 (4) ◽  
pp. 1324-1330 ◽  
Author(s):  
E. Bruton Strange ◽  
Nihat Guner ◽  
Zvezdana Pesic‐VanEsbroeck ◽  
Todd C. Wehner
Keyword(s):  
Virus Type ◽  
Germplasm Collection ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus

scholarly journals Danos causados pelo Zucchini lethal chlorosis virus (ZLCV) sobre a produção de frutos comerciais de abobrinha de moita 'Caserta'

2009 ◽  
Vol 35 (3) ◽  
pp. 223-225 ◽  
Author(s):  
José Segundo Giampan ◽  
Jorge Alberto Marques Rezende ◽  
Sônia Maria De Stefano Piedade
Keyword(s):  
Mosaic Virus ◽  
Virus Type ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Papaya Ringspot Virus

O ZLCV é um tospovírus encontrado com freqüência causando severos danos em cucurbitáceas. Nesse trabalho avaliaram-se os danos causados pelo ZLCV em abobrinha de moita 'Caserta', em campo na ESALQ/USP, Piracicaba-SP, onde esse vírus é freqüente. Plantas obtidas pela semeadura direta foram monitoradas periodicamente quanto à infecção pelo ZLCV por meio dos sintomas e por PTA-ELISA. Monitorou-se ainda a contaminação com Papaya ringspot virus - type W e Zucchini yellow mosaic virus, desconsiderando a produção dessas plantas. As plantas foram agrupadas em função da época de aparecimento dos sintomas do ZLCV, avaliando a produção de frutos comerciais (FC) e não comerciais (FNC) de cada grupo e comparando com a de plantas que permaneceram sem sintomas até o final do experimento. As plantas que apresentaram sintomas até os 23 dias após a emergência (DAE) não produziram qualquer tipo de frutos. FC foram colhidos de plantas que apresentaram sintomas a partir dos 42 DAE. Mesmo assim, houve redução de 78,5 % na produção de FC. Plantas que mostraram sintomas por ocasião da última colheita (55 DAE) apresentaram redução na produção de FC de 9,6 %. A infecção com o ZLCV até o início da frutificação inviabiliza a produção de FC de abobrinha de moita 'Caserta'.

scholarly journals Biomass, virus concentration, and symptomatology of cucurbits infected by mild and severe strains of Papaya ringspot virus

2003 ◽  
Vol 60 (4) ◽  
pp. 691-698 ◽  
Author(s):  
Davi Andrade Pacheco ◽  
Jorge Alberto Marques Rezende ◽  
Sônia Maria de Stefano Piedade
Keyword(s):  
Virus Type ◽  
Aerial Part ◽  
Mosaic Disease ◽  
Ringspot Virus ◽  
Virus Concentration ◽  
Papaya Ringspot Virus ◽  
Severe Strain ◽  
Dry Biomass ◽  
Marketable Fruit ◽  
Cotyledonary Stage

Pre-immunization with mild strains of Papaya ringspot virus - type W (PRWV-W) has allowed the mosaic disease to be controlled in different cucurbit species, with increases in marketable fruit yield. The objective of this study was to compare virus concentration, biomass and symptomatology of 'Caserta' zucchini squash, 'Menina Brasileira' long-neck squash and 'Crimson Sweet' watermelon plants infected by three mild strains and one severe strain of PRSV-W. Plants were inoculated at the cotyledonary stage, under greenhouse conditions, sampled at 7, 14, 21, 28 and 35 days after inoculation (DAI), and analyzed by PTA-ELISA. The severity of the symptoms was scored according to a scale from 1 to 5, and the fresh and dry biomass of the aerial part of the plants were evaluated at 40 DAI. Concentrations of the mild strains, based on absorbance values of the PTA-ELISA, were lower than the concentration of the severe strain for all species. The mild strains did not cause mosaic in infected plants of all species. Plants of zucchini squash and watermelon infected by the severe strain exhibited severe mosaic symptoms, but the same was not noticed for infected long-neck squash plants. Biomass values from zucchini squash and watermelon plants infected by the mild strains were 1.7 % to 12.4 % lower as compared to healthy plants. Biomass values of zucchini squash and watermelon plants infected by the severe strain presented greater reduction, varying from 29 % to 74 %. However, biomass values of long-neck squash plants infected by the mild and severe strains were similar for all treatments.

Screening Carica papaya  ×  C. cauliflora hybrids for resistance to papaya ringspot virus‐type P

1997 ◽  
Vol 46 (6) ◽  
pp. 837-841 ◽  
Author(s):  
P. M. MAGDALITA ◽  
D. M. PERSLEY ◽  
I. D. GODWIN ◽  
R. A. DREW ◽  
S. W. ADKINS
Keyword(s):  
Virus Type ◽  
Carica Papaya ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus

scholarly journals Inheritance of Resistance to the Watermelon Strain of Papaya Ringspot Virus in Watermelon

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 811C-811
Author(s):  
Nihat Guner* ◽  
Zvezdana Pesic-VanEsbroeck ◽  
Todd Wehner
Keyword(s):  
New Hampshire ◽  
Citrullus Lanatus ◽  
Recessive Gene ◽  
Germplasm Collection ◽  
Ringspot Virus ◽  
Inheritance Of Resistance ◽  
Papaya Ringspot Virus ◽  
Single Recessive Gene ◽  
Susceptible Parent ◽  
Sources Of Resistance

Sources of resistance to the watermelon strain of papaya ringspot virus (PRSV-W) have been identified within the watermelon (Citrullus lanatus) germplasm collection. Inheritance of resistance to papaya ringspot virus-watermelon strain was studied in three C. lanatus var. citroides accessions: PI 244017, PI 244019, and PI 485583. The susceptible parent lines `Allsweet', `Calhoun Gray', and `New Hampshire Midget' were crossed with resistant accessions to develop F1, F2, and BC1 generations for six families. A single recessive gene was found to control resistance to PRSV-W. The gene symbol `prv' is proposed for PRSV-W resistance in watermelon. Additional work is needed to determine whether the genes in PI 244017, PI 244019, and PI 485583 are allelic for resistance to PRSV-W.

scholarly journals 032 Resistance to Papaya Ringspot Virus in Watermelon

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 446D-446
Author(s):  
E. Bruton Strange ◽  
Todd C. Wehner ◽  
Zvezdana Pesic-Van Esbroeck
Keyword(s):  
Citrullus Lanatus ◽  
Genetic Resistance ◽  
Germplasm Collection ◽  
Ringspot Virus ◽  
Watermelon Mosaic Virus ◽  
Screening Methods ◽  
Papaya Ringspot Virus ◽  
Virus Diseases ◽  
Sources Of Resistance ◽  
Effective Screening

Watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] is a major crop in the southern U.S., where the most important virus diseases are papaya ringspot virus (PRSV), watermelon mosaic virus-2, and zucchini yellow mosaic. The most economical control of virus diseases of watermelon is probably through genetic resistance. Watermelon has not been screened extensively for resistance to PRSV. The objective of this research was to develop a suitable method for screening watermelons for resistance to PRSV and then to screen the USDA germplasm collection. To date, we have developed an effective method and have nearly completed the screening. Several of the 1283 accessions have shown resistance to the virus. Methods tests involved 10 isolates of PRSV, several watermelon accessions and multiple inoculation procedures. Seedlings were screened in greenhouse flats with six replications per test. Tests were rated visually on a 0 to 9 scale (0 = no damage, 9 = plant dead), as well as with ELISA to detect the presence of virus. The watermelon germplasm collection was screened in four separate runs of 1283 accessions with `Charleston Gray' as the susceptible check. This research will be useful for those interested in effective screening methods, and sources of resistance for development of improved watermelon cultivars.

Detection of Papaya ringspot virus Type W Infecting the Cucurbit Weed Cucumis melo var. dudaim in Florida

2014 ◽  
Vol 15 (1) ◽  
pp. 29-30 ◽  
Author(s):  
Yaowapa Tantiwanich ◽  
Carlye A. Baker ◽  
William W. Turechek ◽  
Scott Adkins
Keyword(s):  
Virus Type ◽  
Cucumis Melo ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
First Report

This is the first report of Papaya ringspot virus type W infecting Cucumis melo var. dudaim, a cucurbit weed, in Florida. It provides an overview of this virus reservoir for growers, extension workers, crop consultants, and research and regulatory scientists. Accepted for publication 21 January 2014. Published 18 March 2014.

scholarly journals First detection of papaya ringspot virus-type W and zucchini yellow mosaic virus infecting Cucurbita maxima in Paraguay

2019 ◽  
Vol 102 (1) ◽  
pp. 231-231
Author(s):  
Arnaldo Esquivel-Fariña ◽  
Viviana Marcela Camelo-García ◽  
Jorge Alberto Marques Rezende ◽  
Elliot Watanabe Kitajima ◽  
Luis Roberto González-Segnana
Keyword(s):  
Mosaic Virus ◽  
Virus Type ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Cucurbita Maxima

Genome analysis of a severe and a mild isolate of Papaya ringspot virus-type W found in Brazil

Virus Genes ◽  
2006 ◽  
Vol 35 (1) ◽  
pp. 119-127 ◽  
Author(s):  
Alice Kazuko Inoue-Nagata ◽  
Carolina de Mello Franco ◽  
Darren Patrick Martin ◽  
Jorge Alberto Marques Rezende ◽  
Gleydson Barros Ferreira ◽  
...  
Keyword(s):  
Virus Type ◽  
Genome Analysis ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Mild Isolate

scholarly journals Danos na produção da abobrinha de moita causados pelo Papaya ringspot virus type W e Zucchini yellow mosaic virus

2007 ◽  
Vol 33 (2) ◽  
pp. 192-194 ◽  
Author(s):  
Mônica Juliani Zavaglia Pereira ◽  
Angelo Aparecido Barbosa Sussel ◽  
Ricardo Ferreira da Silva ◽  
Odair José Kuhn ◽  
Fernanda Domingues ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Type ◽  
Cucurbita Pepo ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Papaya Ringspot Virus

Este trabalho teve por objetivo avaliar, em condições de casa de vegetação e de campo, os danos causados pelo PRSV-W e ZYMV em abobrinha-de-moita (Cucurbita pepo cv. Caserta). As plantas em casa de vegetação foram inoculadas com os vírus individualmente e em mistura aos 12 e 22 dias após emergência (DAE) e aos 5, 15 e 25 DAE no campo. Em casa de vegetação, as infecções com PRSV-W + ZYMV, PRSV-W e ZYMV, na primeira época de inoculação, ocasionaram reduções de área foliar de 39,6%, 36,8% e 12,1%, respectivamente. As massas fresca e seca também foram significativamente afetadas na primeira época de inoculação. No campo, as plantas com infecções individuais ou mistas dos potyvírus produziram frutos não comerciais em quantidades que variaram de 14 a 861 g/planta, dependendo da idade que foram inoculadas. As plantas tratadas com tampão fosfato aos 5, 15 e 25 DAE produziram em média 573 g, 937 g e 1172 g de frutos comerciais e 282 g, 221 g e 192 g de frutos não comerciais, respectivamente. A redução na massa fresca das plantas foi diretamente relacionada com a época de inoculação, com médias de 60,7% para aquelas inoculadas aos 5 DAE e de 22,7% para aquelas inoculadas aos 15 DAE. Na terceira época de inoculação não houve diferença significativa de massa fresca entre os tratamentos.

Nature of resistance in Cucumis melo cvs to papaya ringspot virus type W

1994 ◽  
Vol 45 (3) ◽  
pp. 633 ◽  
Author(s):  
KS Gibb ◽  
AC Padovan ◽  
ME Herrington
Keyword(s):  
Virus Type ◽  
Comparative Studies ◽  
Cucumis Melo ◽  
Ringspot Virus ◽  
Symptom Expression ◽  
Papaya Ringspot Virus ◽  
True Leaf ◽  
Infected Cells ◽  
Systemic Symptoms ◽  
Lines Of Evidence

Comparative studies were made of the reactions of a 'resistant' (Cinco), a susceptible (Planters Jumbo) and a Cinco x Planters Jumbo (Cinbo) cultivar of muskmelon to papaya ringspot virus-watermelon strain (PRSV-W). Neither Cinbo nor Cinco supported levels of virus that were detectable by biotin-ELISA, but PRSV-W was detected in individual cells of the three cultivars. In inoculated leaves of Planters Jumbo most cells were infected, but in both Cinbo and Cinco only 3-7% of cells were infected. When cotyledons of Cinbo were inoculated, mild systemic symptoms were observed, but plants remained symptomless when true leaves were inoculated. Virus was recovered from both cotyledon - inoculated Cinbo grown at 25 or 40�C and true leaf-inoculated Cinbo, but only when the latter were grown at 25�C. There was no difference in the percentage of infected cells in these leaves despite the differences in symptom expression. Cinco was highly resistant and only occasionally showed mild systemic symptoms on cotyledon-inoculated plants grown at 25�C. Unlike those of Cinbo, leaves with symptoms were not infective, but there was no difference in the percentage of infected cells in these leaves compared to those in Cinbo. For both Cinbo and Cinco, several lines of evidence are presented that suggest PRSV-W is restricted to a few cells throughout the plant.

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