Detection of Papaya ringspot virus Type W Infecting the Cucurbit Weed Cucumis melo var. dudaim in Florida

2014 ◽  
Vol 15 (1) ◽  
pp. 29-30 ◽  
Author(s):  
Yaowapa Tantiwanich ◽  
Carlye A. Baker ◽  
William W. Turechek ◽  
Scott Adkins
Keyword(s):  
Virus Type ◽  
Cucumis Melo ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
First Report

This is the first report of Papaya ringspot virus type W infecting Cucumis melo var. dudaim, a cucurbit weed, in Florida. It provides an overview of this virus reservoir for growers, extension workers, crop consultants, and research and regulatory scientists. Accepted for publication 21 January 2014. Published 18 March 2014.

Nature of resistance in Cucumis melo cvs to papaya ringspot virus type W

1994 ◽  
Vol 45 (3) ◽  
pp. 633 ◽  
Author(s):  
KS Gibb ◽  
AC Padovan ◽  
ME Herrington
Keyword(s):  
Virus Type ◽  
Comparative Studies ◽  
Cucumis Melo ◽  
Ringspot Virus ◽  
Symptom Expression ◽  
Papaya Ringspot Virus ◽  
True Leaf ◽  
Infected Cells ◽  
Systemic Symptoms ◽  
Lines Of Evidence

Comparative studies were made of the reactions of a 'resistant' (Cinco), a susceptible (Planters Jumbo) and a Cinco x Planters Jumbo (Cinbo) cultivar of muskmelon to papaya ringspot virus-watermelon strain (PRSV-W). Neither Cinbo nor Cinco supported levels of virus that were detectable by biotin-ELISA, but PRSV-W was detected in individual cells of the three cultivars. In inoculated leaves of Planters Jumbo most cells were infected, but in both Cinbo and Cinco only 3-7% of cells were infected. When cotyledons of Cinbo were inoculated, mild systemic symptoms were observed, but plants remained symptomless when true leaves were inoculated. Virus was recovered from both cotyledon - inoculated Cinbo grown at 25 or 40�C and true leaf-inoculated Cinbo, but only when the latter were grown at 25�C. There was no difference in the percentage of infected cells in these leaves despite the differences in symptom expression. Cinco was highly resistant and only occasionally showed mild systemic symptoms on cotyledon-inoculated plants grown at 25�C. Unlike those of Cinbo, leaves with symptoms were not infective, but there was no difference in the percentage of infected cells in these leaves compared to those in Cinbo. For both Cinbo and Cinco, several lines of evidence are presented that suggest PRSV-W is restricted to a few cells throughout the plant.

scholarly journals First Report of Papaya ringspot virus-Type W Infecting Fevillea Species (Cucurbitaceae) in South America

Plant Disease ◽  
2016 ◽  
Vol 100 (12) ◽  
pp. 2540-2540 ◽  
Author(s):  
G. M. de Castro ◽  
M. F. Lima ◽  
M. E. N. Fonseca ◽  
L. S. Boiteux ◽  
F. P. Lobo ◽  
...  
Keyword(s):  
South America ◽  
Virus Type ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
First Report

Screening the Watermelon Germplasm Collection for Resistance to Papaya Ringspot Virus Type‐W

Crop Science ◽  
2002 ◽  
Vol 42 (4) ◽  
pp. 1324-1330 ◽  
Author(s):  
E. Bruton Strange ◽  
Nihat Guner ◽  
Zvezdana Pesic‐VanEsbroeck ◽  
Todd C. Wehner
Keyword(s):  
Virus Type ◽  
Germplasm Collection ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus

scholarly journals Danos causados pelo Zucchini lethal chlorosis virus (ZLCV) sobre a produção de frutos comerciais de abobrinha de moita 'Caserta'

2009 ◽  
Vol 35 (3) ◽  
pp. 223-225 ◽  
Author(s):  
José Segundo Giampan ◽  
Jorge Alberto Marques Rezende ◽  
Sônia Maria De Stefano Piedade
Keyword(s):  
Mosaic Virus ◽  
Virus Type ◽  
Zucchini Yellow Mosaic Virus ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Papaya Ringspot Virus

O ZLCV é um tospovírus encontrado com freqüência causando severos danos em cucurbitáceas. Nesse trabalho avaliaram-se os danos causados pelo ZLCV em abobrinha de moita 'Caserta', em campo na ESALQ/USP, Piracicaba-SP, onde esse vírus é freqüente. Plantas obtidas pela semeadura direta foram monitoradas periodicamente quanto à infecção pelo ZLCV por meio dos sintomas e por PTA-ELISA. Monitorou-se ainda a contaminação com Papaya ringspot virus - type W e Zucchini yellow mosaic virus, desconsiderando a produção dessas plantas. As plantas foram agrupadas em função da época de aparecimento dos sintomas do ZLCV, avaliando a produção de frutos comerciais (FC) e não comerciais (FNC) de cada grupo e comparando com a de plantas que permaneceram sem sintomas até o final do experimento. As plantas que apresentaram sintomas até os 23 dias após a emergência (DAE) não produziram qualquer tipo de frutos. FC foram colhidos de plantas que apresentaram sintomas a partir dos 42 DAE. Mesmo assim, houve redução de 78,5 % na produção de FC. Plantas que mostraram sintomas por ocasião da última colheita (55 DAE) apresentaram redução na produção de FC de 9,6 %. A infecção com o ZLCV até o início da frutificação inviabiliza a produção de FC de abobrinha de moita 'Caserta'.

scholarly journals Biomass, virus concentration, and symptomatology of cucurbits infected by mild and severe strains of Papaya ringspot virus

2003 ◽  
Vol 60 (4) ◽  
pp. 691-698 ◽  
Author(s):  
Davi Andrade Pacheco ◽  
Jorge Alberto Marques Rezende ◽  
Sônia Maria de Stefano Piedade
Keyword(s):  
Virus Type ◽  
Aerial Part ◽  
Mosaic Disease ◽  
Ringspot Virus ◽  
Virus Concentration ◽  
Papaya Ringspot Virus ◽  
Severe Strain ◽  
Dry Biomass ◽  
Marketable Fruit ◽  
Cotyledonary Stage

Pre-immunization with mild strains of Papaya ringspot virus - type W (PRWV-W) has allowed the mosaic disease to be controlled in different cucurbit species, with increases in marketable fruit yield. The objective of this study was to compare virus concentration, biomass and symptomatology of 'Caserta' zucchini squash, 'Menina Brasileira' long-neck squash and 'Crimson Sweet' watermelon plants infected by three mild strains and one severe strain of PRSV-W. Plants were inoculated at the cotyledonary stage, under greenhouse conditions, sampled at 7, 14, 21, 28 and 35 days after inoculation (DAI), and analyzed by PTA-ELISA. The severity of the symptoms was scored according to a scale from 1 to 5, and the fresh and dry biomass of the aerial part of the plants were evaluated at 40 DAI. Concentrations of the mild strains, based on absorbance values of the PTA-ELISA, were lower than the concentration of the severe strain for all species. The mild strains did not cause mosaic in infected plants of all species. Plants of zucchini squash and watermelon infected by the severe strain exhibited severe mosaic symptoms, but the same was not noticed for infected long-neck squash plants. Biomass values from zucchini squash and watermelon plants infected by the mild strains were 1.7 % to 12.4 % lower as compared to healthy plants. Biomass values of zucchini squash and watermelon plants infected by the severe strain presented greater reduction, varying from 29 % to 74 %. However, biomass values of long-neck squash plants infected by the mild and severe strains were similar for all treatments.

scholarly journals First Report of Papaya ringspot virus Associated With a Ringspot Disease of Zucchini in Northern China

Plant Disease ◽  
2017 ◽  
Vol 101 (5) ◽  
pp. 847 ◽  
Author(s):  
D.-J. Cheng ◽  
X.-D. Huang ◽  
J.-W. Zhang ◽  
Y.-P. Tian ◽  
X.-Y. Wang ◽  
...  
Keyword(s):  
Northern China ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
First Report

Screening Carica papaya  ×  C. cauliflora hybrids for resistance to papaya ringspot virus‐type P

1997 ◽  
Vol 46 (6) ◽  
pp. 837-841 ◽  
Author(s):  
P. M. MAGDALITA ◽  
D. M. PERSLEY ◽  
I. D. GODWIN ◽  
R. A. DREW ◽  
S. W. ADKINS
Keyword(s):  
Virus Type ◽  
Carica Papaya ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus

scholarly journals First Report of Papaya ringspot virus and Zucchini yellow mosaic virus in Luohanguo (Siraitia grosvenorii) in China

Plant Disease ◽  
2005 ◽  
Vol 89 (5) ◽  
pp. 530-530 ◽  
Author(s):  
Y.-M. Liao ◽  
X.-J. Gan ◽  
B. Chen ◽  
J.-H. Cai
Keyword(s):  
Mosaic Virus ◽  
Zucchini Yellow Mosaic Virus ◽  
Viral Disease ◽  
Ringspot Virus ◽  
Yellow Mosaic Virus ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Rt Pcr ◽  
First Report ◽  
Siraitia Grosvenorii

Luohanguo, Siraitia grosvenorii (Swingle) C. Jeffrey, is a perennial cucurbitaceous plant that is an economically important medicinal and sweetener crop in Guangxi province, China. Surveys conducted during the summer to fall seasons of 2003-2004 in northern Guangxi showed symptoms typical of a viral disease, including leaf mottling, mosaic, vein clearing, curling, and shoestring-like distortion in the field. Mechanical inoculation of sap from leaves of symptomatic plants collected from the surveyed areas caused similar symptoms on tissue culture-derived healthy Luohanguo plants. Two sequences of 0.7 and 1.6 kb with 88 and 97% identity to Papaya ringspot virus (PRSV) and Zucchini yellow mosaic virus (ZYMV) were amplified using reverse transcription-polymerase chain reaction (RT-PCR) with purified flexuous viral particles or total RNA extracted from the symptomatic Luohanguo leaves as templates with conserved degenerate potyvirus primers (1). To confirm the results, primers specific for PRSV (PP1/PP2, genome coordinates 4064-4083/5087-5069, GenBank Accession No X97251) and ZYMV (ZP1/ZP2, genome coordinates 5540-5557/7937-7920, GenBank Accession No L31350) were used to perform RT-PCR from the same RNA templates. The expected 1.0- and 2.3-kb fragments were amplified and they were 90 and 95% identical to PRSV and ZYMV in sequence, respectively. Watermelon mosaic virus was not detected. To our knowledge, this is the first report of the occurrence of PRSV and ZYMV in Luohanguo. Reference: (1) A. Gibbs et al. J. Virol. Methods 63:9, 1997.

scholarly journals 149 DIFFERENTIAL REACTIONS OF MELONS TO ISOLATES OF PAPAYA RINGSPOT VIRUS WATERMELON STRAIN

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 450b-450
Author(s):  
Patti Fashing-Burdette ◽  
James D. McCreight
Keyword(s):  
Mosaic Virus ◽  
Cucumis Melo ◽  
Ringspot Virus ◽  
Watermelon Mosaic Virus ◽  
Papaya Ringspot Virus ◽  
Plant Introductions ◽  
Local Lesions ◽  
Necrotic Spots ◽  
Incompatible Reaction

In 1963, melon (Cucumis melo L.) plant introductions (PI) 124112 and PI 180280 were reported variable in response to inoculation with the T-1 or Freitag's isolates of watermelon mosaic virus. Most plants were symptomless, but some had small pinpoint necrotic lesions on cotyledons and leaves. The T-1 and Freitag's isolates of watermelon mosaic were later designated watermelon mosaic virus 1, and more recently renamed papaya ringspot virus watermelon strain (PRSV-W). When inoculated with California or Florida isolates of PRSV-W in 1993, WMR 29 a western U.S. shipping type melon derived from PI 180280 was symptomless (incompatible reaction) and SDS-immunodiffusion assays were negative. In contrast when inoculated with the same PRSV-W isolates, PI 124112 had incompatible reactions characterized by wilting, local lesions, systemic necrotic spots and necrosis and negative SDS-immunodiffusion assays.

scholarly journals Melon PI 414723 Is Resistant to Papaya Ringspot Virus Watermelon Strain

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 602b-602
Author(s):  
James D. McCreight
Keyword(s):  
Cucumis Melo ◽  
Powdery Mildew Resistance ◽  
Aphis Gossypii ◽  
Dominant Gene ◽  
Uttar Pradesh ◽  
Virus Multiplication ◽  
Ringspot Virus ◽  
Papaya Ringspot Virus ◽  
Systemic Necrosis ◽  
Melon Aphid

PI 414723 has received much attention from melon (Cucumis melo L.) breeders, pathologists, and entomologists for resistances to zucchini yellow mosaic and watermelon mosaic viruses, including resistances to virus multiplication and subsequent transmission by the melon aphid, powdery mildew resistance, and melon aphid (Aphis gossypii Glover). PI 414723 was derived from PI 371795, which was a contaminant in cucumber (Cucumis sativus L.) PI 175111 collected in 1948 by Walter N. Koelz in Mussoorie, Uttar Pradesh, India (altitude 1829 m). Its fruit, which have soft flesh and rind that split at maturity, are used in soups and stews, and the seeds are roasted and eaten. PI 414723, PI 371795, and the related Ames 20219 and progeny 92528a were resistant to California and Florida isolates of papaya ringspot virus watermelon strain (PRSV-W). Plants were either symptomless, or they exhibited local lesions, systemic necrosis, or systemic spots. Resistance to PRSV-W is conditioned by a single dominant gene. Allelism with Prv1 (PI 180280, Rajkot, Gujarat, India), Prv2 (PI 180283, Bhavnagar, Gujarat, India), Nm (`Vedrantais, Fance), and a recently described gene for PRSV-W resistance in PI 124112 (Calcutta, India) is yet to be determined.

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