Differential effects of single-walled carbon nanotubes on cell viability of human lung and pharynx carcinoma cell lines

2011 ◽  
Vol 36 (3) ◽  
pp. 379-387 ◽  
Author(s):  
Kotaro Hitoshi ◽  
Miki Katoh ◽  
Tomoko Suzuki ◽  
Yoshinori Ando ◽  
Masayuki Nadai
Keyword(s):  
Carbon Nanotubes ◽  
Cell Viability ◽  
Cell Lines ◽  
Human Lung ◽  
Carcinoma Cell ◽  
Single Walled Carbon Nanotubes ◽  
Differential Effects ◽  
Single Walled Carbon ◽  
Carcinoma Cell Lines ◽  
Walled Carbon Nanotubes

scholarly journals Single-Walled Carbon Nanotubes Attenuate Cytotoxic and Oxidative Stress Response of Pb in Human Lung Epithelial (A549) Cells

2020 ◽  
Vol 17 (21) ◽  
pp. 8221
Author(s):  
Maqusood Ahamed ◽  
Mohd Javed Akhtar ◽  
M. A. Majeed Khan
Keyword(s):  
Carbon Nanotubes ◽  
Human Lung ◽  
A549 Cells ◽  
Single Walled Carbon Nanotubes ◽  
Ambient Air ◽  
Combined Effects ◽  
X Ray ◽  
Single Walled Carbon ◽  
Lung Epithelial ◽  
Walled Carbon Nanotubes

Combined exposure of single-walled carbon nanotubes (SWCNTs) and trace metal lead (Pb) in ambient air is unavoidable. Most of the previous studies on the toxicity of SWCNTs and Pb have been conducted individually. There is a scarcity of information on the combined toxicity of SWCNTs and Pb in human cells. This work was designed to explore the combined effects of SWCNTs and Pb in human lung epithelial (A549) cells. SWCNTs were prepared through the plasma-enhanced vapor deposition technique. Prepared SWCNTs were characterized by x-ray diffraction, x-ray photoelectron spectroscopy, scanning electron microscopy, and dynamic light scattering. We observed that SWCNTs up to a concentration of 100 µg/mL was safe, while Pb induced dose-dependent (5–100 µg/mL) cytotoxicity in A549 cells. Importantly, cytotoxicity, cell cycle arrest, mitochondrial membrane potential depletion, lipid peroxidation, and induction of caspase-3 and -9 enzymes following Pb exposure (50 µg/mL for 24 h) were efficiently attenuated by the co-exposure of SWCNTs (10 µg/mL for 24 h). Furthermore, generation of Pb-induced pro-oxidants (reactive oxygen species and hydrogen peroxide) and the reduction of antioxidants (antioxidant enzymes and glutathione) were also mitigated by the co-exposure of SWCNTs. Inductively coupled plasma-mass spectrometry results suggest that the adsorption of Pb on the surface of SWCNTs could attenuate the bioavailability and toxicity of Pb in A549 cells. Our data warrant further research on the combined effects of SWCNTs and Pb in animal models.

Interactions and effects of BSA-functionalized single-walled carbon nanotubes on different cell lines

2016 ◽  
Vol 27 (15) ◽  
pp. 155704 ◽  
Author(s):  
Laura Muzi ◽  
Franco Tardani ◽  
Camillo La Mesa ◽  
Adalberto Bonincontro ◽  
Alberto Bianco ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Cell Lines ◽  
Single Walled Carbon Nanotubes ◽  
Single Walled Carbon ◽  
Walled Carbon Nanotubes

A Comparable Study on the Effects of Pristine and Functionalized Single-Walled Carbon Nanotubes on Schwann Cells

2014 ◽  
Vol 886 ◽  
pp. 63-65
Author(s):  
Tong Qiu
Keyword(s):  
Carbon Nanotubes ◽  
Antioxidant Activity ◽  
Cell Viability ◽  
Schwann Cells ◽  
Single Walled Carbon Nanotubes ◽  
Antioxidant Ability ◽  
Redox States ◽  
Single Walled Carbon ◽  
Walled Carbon Nanotubes ◽  
Carboxyl Modification

A comparable study on the effects of pristine and functionalized single-walled carbon nanotubes on schwann cells were conducted. Schwann cells were co-cultured with three types of SWNTs, purified raw SWNTs (C), hydroxyl purified SWNTs (C-OH) and carboxyl purified SWNTs (C-COOH) at 25μg/mL. C-COOH promoted the cell viability after 72h exposure and sustained its redox states by enhancing GPx expression. On the contrary, C and C-OH weaken the antioxidant activity due to the inhibition in main antioxidant enzymes and further led to the decline in cell viability. Carboxyl modification of the SWNTs helps to improve the biocompatibility of the SWNTs by strengthening the antioxidant ability.

In Vitro Cytotoxicity of Multiwalled and Single-Walled Carbon Nanotubes on Human Cell Lines

2014 ◽  
Vol 23 (5) ◽  
pp. 377-382 ◽  
Author(s):  
Shweta Arora ◽  
Harmanmeet Kaur ◽  
Rajendra Kumar ◽  
Rupinder Kaur ◽  
Deepa Rana ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Cell Lines ◽  
Human Cell ◽  
Single Walled Carbon Nanotubes ◽  
In Vitro Cytotoxicity ◽  
Human Cell Lines ◽  
Single Walled Carbon ◽  
Walled Carbon Nanotubes

scholarly journals Poly-dispersed-acid-functionalized-single-walled carbon nanotubes (AF-SWCNTs) are potent inhibitor of BCG induced inflammatory response in macrophage cell lines

2020 ◽  
Author(s):  
Deepika Bhardwaj ◽  
Rajiv K. Saxena
Keyword(s):  
Carbon Nanotubes ◽  
Inflammatory Response ◽  
Cell Lines ◽  
Single Walled Carbon Nanotubes ◽  
Transcriptional Level ◽  
Macrophage Cell ◽  
Single Walled Carbon ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Walled Carbon Nanotubes

AbstractPresent study is focussed on the modulation of Mycobacterium bovis BCG induced inflammatory response by poly-dispersed acid-functionalized single-walled carbon nanotubes (AF-SWCNTs) in macrophages. Flow cytometric and confocal microscopy studies indicated that both BCG and AF-SWCNTs were efficiently internalized by RAW 264.7 and MH-S macrophage cell lines and were essentially localized in the cytoplasmic area. The results indicated strong antioxidant activity of AF-SWCNTs in mitigating BCG induced oxidative and nitrosative stress. We also found a marked decline in expression of BCG induced pro-inflammatory genes like COX-2, iNOS, TNF-α, IL-6 and IL-1β on treatment with AF-SWCNTs at transcriptional level. Decline in expression of BCG induced COX-2 by AF-SWCNTs was also confirmed at protein level using Western blotting. Anti-inflammatory activity of AF-SWCNTs was further validated by our results showing that AF-SWCNTs treatment induced a precipitous decline in BCG induced release of Matrix Metalloproteinases MMP-2 and MMP-9 by macrophage cell lines, by using Gelatin zymography. Taken together, our results demonstrate potent anti-inflammatory role of AF-SWCNTs in alleviating BCG induced inflammation.

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