Single-walled carbon nanotubes (SWCNTs) inhibit heat shock protein 90 (HSP90) signaling in human lung fibroblasts and keratinocytes

2017 ◽  
Vol 329 ◽  
pp. 347-357 ◽  
Author(s):  
Li-Chu Ong ◽  
Yuen-Fen Tan ◽  
Boon Shing Tan ◽  
Felicia Fei-Lei Chung ◽  
Soon-Keng Cheong ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Human Lung ◽  
Heat Shock Protein 90 ◽  
Single Walled Carbon Nanotubes ◽  
Lung Fibroblasts ◽  
Human Lung Fibroblasts ◽  
Single Walled Carbon ◽  
Walled Carbon Nanotubes

scholarly journals Single-Walled Carbon Nanotubes Attenuate Cytotoxic and Oxidative Stress Response of Pb in Human Lung Epithelial (A549) Cells

2020 ◽  
Vol 17 (21) ◽  
pp. 8221
Author(s):  
Maqusood Ahamed ◽  
Mohd Javed Akhtar ◽  
M. A. Majeed Khan
Keyword(s):  
Carbon Nanotubes ◽  
Human Lung ◽  
A549 Cells ◽  
Single Walled Carbon Nanotubes ◽  
Ambient Air ◽  
Combined Effects ◽  
X Ray ◽  
Single Walled Carbon ◽  
Lung Epithelial ◽  
Walled Carbon Nanotubes

Combined exposure of single-walled carbon nanotubes (SWCNTs) and trace metal lead (Pb) in ambient air is unavoidable. Most of the previous studies on the toxicity of SWCNTs and Pb have been conducted individually. There is a scarcity of information on the combined toxicity of SWCNTs and Pb in human cells. This work was designed to explore the combined effects of SWCNTs and Pb in human lung epithelial (A549) cells. SWCNTs were prepared through the plasma-enhanced vapor deposition technique. Prepared SWCNTs were characterized by x-ray diffraction, x-ray photoelectron spectroscopy, scanning electron microscopy, and dynamic light scattering. We observed that SWCNTs up to a concentration of 100 µg/mL was safe, while Pb induced dose-dependent (5–100 µg/mL) cytotoxicity in A549 cells. Importantly, cytotoxicity, cell cycle arrest, mitochondrial membrane potential depletion, lipid peroxidation, and induction of caspase-3 and -9 enzymes following Pb exposure (50 µg/mL for 24 h) were efficiently attenuated by the co-exposure of SWCNTs (10 µg/mL for 24 h). Furthermore, generation of Pb-induced pro-oxidants (reactive oxygen species and hydrogen peroxide) and the reduction of antioxidants (antioxidant enzymes and glutathione) were also mitigated by the co-exposure of SWCNTs. Inductively coupled plasma-mass spectrometry results suggest that the adsorption of Pb on the surface of SWCNTs could attenuate the bioavailability and toxicity of Pb in A549 cells. Our data warrant further research on the combined effects of SWCNTs and Pb in animal models.

Purity Evaluation of Single-Walled Carbon Nanotubes Using Thermogravimetric Analysis

2013 ◽  
Vol 51 (2) ◽  
pp. 137-144
Author(s):  
Naesung Lee ◽  
Jeung Choon Goak ◽  
Tae Yang Kim ◽  
Jongwan Jung ◽  
Young-Soo Seo ◽  
...  
Keyword(s):  
Carbon Nanotubes ◽  
Thermogravimetric Analysis ◽  
Single Walled Carbon Nanotubes ◽  
Single Walled Carbon ◽  
Purity Evaluation ◽  
Walled Carbon Nanotubes
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