scholarly journals Strain differences in hepatic cytochrome P450 1A and 3A expression between Sprague-Dawley and Wistar rats

2008 ◽  
Vol 33 (4) ◽  
pp. 447-457 ◽  
Author(s):  
Tomoyuki Kishida ◽  
Shin-ichi Muto ◽  
Morimichi Hayashi ◽  
Masaru Tsutsui ◽  
Satoru Tanaka ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Wistar Rats ◽  
Strain Differences ◽  
Sprague Dawley ◽  
Cytochrome P450 1A ◽  
Hepatic Cytochrome

Strain Differences Occurring in an Experimental Study of Punishment

1965 ◽  
Vol 16 (2) ◽  
pp. 531-536 ◽  
Author(s):  
Morton H. Kleban
Keyword(s):  
Experimental Study ◽  
Empirical Evidence ◽  
Wistar Rats ◽  
Strain Differences ◽  
Training Procedure ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Y Maze ◽  
Minimum Number ◽  
The Difference

Forty-three Sprague-Dawley and 43 Wistar rats were given reward training for 40 trials in a Y-maze. On the next 20 trials, control groups were continued under the same training procedure, and 50% shock trials were introduced in the training of the remaining rats. For the extinction training, the reward was shifted to the opposite arm and 50% shock was continued for the no-delay and 30-sec. delay shock groups. The most significant results were that in the 30-sec. delay groups, the delay helped the Sprague-Dawley rats reverse in a minimum number of trials, whereas the Wistar rats showed strong indications of response stereotypy. The findings with respect to the Sprague-Dawley rats supported the empirical evidence on the effectiveness of delay in overcoming response persistence and the findings on the Wistar rats supported the empirical evidence on omission in punishment. The difference in response to punishment between the two albino strains emphasizes the need for experimental study of strain factors. Experiments should be repeated with several animal strains to remedy over-generalization from single strains and to help elaborate our understanding of the interaction present between punishment and strains.

scholarly journals Carbonated soft drinks alter hepatic cytochrome P450 isoform expression in Wistar rats

2016 ◽  
Vol 5 (5) ◽  
pp. 607-612 ◽  
Author(s):  
Adel Alkhedaide ◽  
Mohamed Mohamed Soliman ◽  
Zein Shaban Ibrahim
Keyword(s):  
Cytochrome P450 ◽  
Wistar Rats ◽  
Soft Drinks ◽  
Carbonated Soft Drinks ◽  
Isoform Expression ◽  
Hepatic Cytochrome

Induction of cytochrome p450 1a and 2b by α-and β-lonone in sprague dawley rats

2002 ◽  
Vol 25 (2) ◽  
pp. 197-201 ◽  
Author(s):  
Hye Gwang Jeong ◽  
Young-Jin Chun ◽  
Chul-Ho Yun ◽  
Chang-Kiu Moon ◽  
Hye-Sook Lee ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Cytochrome P450 1A

scholarly journals Strain differences in purified rat hepatic 3 α-hydroxysteroid UDP-glucuronosyltransferase

1985 ◽  
Vol 230 (2) ◽  
pp. 403-409 ◽  
Author(s):  
M D Green ◽  
C N Falany ◽  
R B Kirkpatrick ◽  
T R Tephly
Keyword(s):  
Individual Differences ◽  
Chenodeoxycholic Acid ◽  
Wistar Rats ◽  
Glucuronic Acid ◽  
Strain Differences ◽  
Sprague Dawley ◽  
Qualitative And Quantitative ◽  
Sprague Dawley Rats ◽  
Different Populations ◽  
Udp Glucuronosyltransferase

Qualitative and quantitative differences of purified hepatic 3 α-hydroxysteroid UDP-glucuronosyltransferase were investigated in Wistar and Sprague-Dawley rats. Individual differences in the glucuronidation rate of androsterone and chenodeoxycholic acid were observed in hepatic microsomal fractions from Wistar but not Sprague-Dawley rats. No individual variation was observed in the glucuronidation of testosterone, p-nitrophenol or oestrone. The 3 α-hydroxysteroid UDP-glucuronosyltransferases from livers of Wistar and Sprague-Dawley rats were isolated and highly purified by using Chromatofocusing and affinity chromatography. The amount of 3 α-hydroxysteroid UDP-glucuronosyltransferase in the liver of Wistar rats exhibiting low rates for androsterone glucuronidation is about 10% or less than that found in hepatic microsomal fractions obtained from Wistar rats having high rates for androsterone glucuronidation. The apparent Km for androsterone with purified 3 α-hydroxysteroid UDP-glucuronosyltransferase from Wistar rats with high glucuronidation activity (6 microM) was not different from that observed for the enzyme purified from Sprague-Dawley animals, whereas that for the enzyme purified from Wistar rats with low glucuronidation activity was substantially higher (120 microM). Despite the differences in apparent Km values for androsterone, the apparent Km for UDP-glucuronic acid (0.3 mM) was not different in the different populations of rats.

scholarly journals Effects of Δ9-tetrahydrocannabinol (THC) vapor inhalation in Sprague-Dawley and Wistar rats

2019 ◽  
Author(s):  
Michael A. Taffe ◽  
K. M. Creehan ◽  
Sophia A. Vandewater ◽  
Tony M. Kerr ◽  
Maury Cole
Keyword(s):  
Propylene Glycol ◽  
Plasma Levels ◽  
Intraperitoneal Injection ◽  
Wistar Rats ◽  
Indirect Comparison ◽  
Strain Differences ◽  
Sprague Dawley ◽  
Differential Impact ◽  
Sd Rats ◽  
Dose Dependent

AbstractA novel inhalation system based on e-cigarette technology has been recently shown to produce hypothermic and anti-nociceptive effects of Δ9-tetrahydrocannabinol (THC) in rats. Indirect comparison of some prior investigations suggested differential impact of inhaled THC between Wistar (WI) and Sprague-Dawley (SD) rats, thus this study was conducted to directly compare the strains.Groups (N=8 per strain) of age matched male SD and WI rats were prepared with radiotelemetry devices to measure temperature and then exposed to vapor from the propylene glycol (PG) vehicle or THC (25, 100, 200 mg/mL of PG) for 30 or 40 minutes. Additional studies evaluated plasma THC levels and anti-nociceptive effects after THC inhalation as well as the thermoregulatory effect of intraperitoneal injection of THC (5-30 mg/kg).Hypothermic effects of inhaled THC was more pronounced in SD rats however plasma levels of THC were identical across strains under either fixed inhalation conditions or injection of a mg/kg equivalent dose. Strain differences in hypothermia were even more pronounced after i.p. injection of THC with SD rats exhibiting dose-dependent temperature reduction after 5 or 10 mg/kg, i.p. and the WI rats only exhibiting significant hypothermia after 20 mg/kg, i.p. The anti-nociceptive effects of inhaled THC (100, 200 mg/mL) did not differ significantly across the strains. These studies confirm an insensitivity of WI rats, compared with SD rats, to the hypothermia induced by THC following inhalation conditions that produced identical plasma THC and anti-nociception. Thus strain differences were not due to differential THC delivery via vapor inhalation.

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