scholarly journals Repeated 28-day and 13-week dose toxicity studies of oils prepared from the internal organs of the Japanese giant scallop (Patinopecten yessoensis) in rats

2020 ◽  
Vol 7 (4) ◽  
pp. 177-188
Author(s):  
Koki Sugimoto ◽  
Eito Shimizu ◽  
Nozomi Hagihara ◽  
Ryota Hosomi ◽  
Kenji Fukunaga ◽  
...  
Keyword(s):  
Internal Organs ◽  
Patinopecten Yessoensis ◽  
Toxicity Studies

scholarly journals Single and Repeated Dose 28-Day and 13-Week Toxicity Studies of Oil Prepared from the Internal Organs of the Japanese Giant Scallop (Patinopecten yessoensis) in Mice

Foods ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 691
Author(s):  
Koki Sugimoto ◽  
Toshifumi Tanizaki ◽  
Eito Shimizu ◽  
Ryota Hosomi ◽  
Kenji Fukunaga ◽  
...  
Keyword(s):  
Oral Dose ◽  
Clinical Signs ◽  
Toxicity Study ◽  
Toxic Substances ◽  
Food Ingredient ◽  
Internal Organs ◽  
Experimental Conditions ◽  
Patinopecten Yessoensis ◽  
Food Ingredients ◽  
Toxicity Studies

Internal organs of discarded scallops are rich in omega-3 polyunsaturated fatty acids, but it is not used as a food ingredient due to the presence of toxic substances. Recently, our research team prepared high-quality scallop oil (SCO) from the internal organs of the Japanese giant scallop (Patinopecten yessoensis), in which cadmium and diarrhetic shellfish toxin are below regulated levels. In this study, SCO was prepared from the internal organs of scallops obtained from Mutsu and Uchiura bays in Japan, and was referred to as SCO-M (scallop oil from Mutsu bay) and SCO-U (scallop oil from Uchiura bay), respectively. Acute and subacute toxicity studies were performed to assess the safety of the prepared SCO. In acute toxicity study, mice were orally administered SCO-M and SCO-U at a single dose of 5,000 mg/kg body weight. In a 28-day repeated oral dose toxicity study, the mice were fed diets containing 1% and 5% SCO-M and SCO-U; and in a 13-week repeated oral dose toxicity study, the mice were fed 5% SCO-M and SCO-U. There were no toxicologically significant changes in clinical signs, hematology, blood chemistry, and organ weights at any dose during the experiment. Therefore, it was concluded that SCO-M and SCO-U are safe for use as food ingredients under the experimental conditions of this study.

scholarly journals Genotoxicity evaluation of oil prepared from the internal organs of the Japanese giant scallop (Patinopecten yessoensis)

2019 ◽  
Vol 6 (4) ◽  
pp. 137-143
Author(s):  
Koki Sugimoto ◽  
Ryota Hosomi ◽  
Kenji Fukunaga ◽  
Takaki Shimono ◽  
Seiji Kanda ◽  
...  
Keyword(s):  
Internal Organs ◽  
Patinopecten Yessoensis

scholarly journals Dietary Phospholipids Prepared From Scallop Internal Organs Attenuate the Serum and Liver Cholesterol Contents by Enhancing the Expression of Cholesterol Hydroxylase in the Liver of Mice

2021 ◽  
Vol 8 ◽  
Author(s):  
Koki Sugimoto ◽  
Ryota Hosomi ◽  
Munehiro Yoshida ◽  
Kenji Fukunaga
Keyword(s):  
Acid Synthesis ◽  
Bile Acid Synthesis ◽  
Farnesoid X Receptor ◽  
Liver Cholesterol ◽  
Internal Organs ◽  
Patinopecten Yessoensis ◽  
Cholesterol Lowering ◽  
Lowering Effect ◽  
Fa Composition ◽  
Cholesterol Lowering Effect

In this study, we successfully prepared scallop oil (SCO), which contains high levels of phospholipids (PL) and eicosapentaenoic acid (EPA), from the internal organs of the Japanese giant scallop (Patinopecten yessoensis), one of the most important underutilized fishery resources in Japan. The intake of SCO lowers the serum and liver cholesterol contents in mice; however, whether the fatty acids (FA) composition or PL of SCO exhibits any cholesterol-lowering effect remains unknown. To elucidate whether the cholesterol-lowering function is due to FA composition or PL of SCO, and investigate the cholesterol-lowering mechanism by SCO, in the present study, mice were fed SCO's PL fraction (SCO-PL), triglyceride (TG)-type oil with almost the same FA composition as SCO-PL, called SCO's TG fraction (SCO-TG), soybean oil (SOY-TG), and soybean's PL fraction (SOY-PL). Male C57BL/6J mice (5-week-old) were fed high-fat and cholesterol diets containing 3% (w/w) experimental oils (SOY-TG, SOY-PL, SCO-TG, and SCO-PL) for 28 days. The SCO-PL diet significantly decreased the serum and liver cholesterol contents compared with the SOY-TG diet, but the intake of SOY-PL and SCO-TG did not show this effect. This result indicated that the serum and liver cholesterol-lowering effect observed in the SCO intake group was due to the effect of SCO-PL. The cholesterol-lowering effect of SCO-PL was in part related to the promotion of liver cholesterol 7α-hydroxylase (CYP7A1) expression, which is the rate-limiting enzyme for bile acid synthesis. In contrast, the expression levels of the ileum farnesoid X receptor (Fxr) and fibroblast growth factor 15 (Fgf15), which inhibit the expression of liver CYP7A1, were significantly reduced in the SCO-PL group than the SOY-TG group. From these results, the increase in the liver CYP7A1 expression by dietary SCO-PL was in part through the reduction of the ileum Fxr/Fgf15 regulatory pathway. Therefore, this study showed that SCO-PL may be a health-promoting component as it lowers the serum and liver cholesterol contents by increasing the liver CYP7A1 expression, which is not seen in SOY-PL and SCO-TG.

Preclinical Toxicity Studies of Naltrexone

1976 ◽  
Author(s):  
Monique C. Braude ◽  
J. Michael Morrison
Keyword(s):  
Toxicity Studies

Human liver cell cultivation for long-term in vitro toxicity studies in a miniaturized multi-compartment 3D bioreactor system

2011 ◽  
Vol 49 (01) ◽  
Author(s):  
SA Hoffmann ◽  
M Lübberstedt ◽  
U Müller-Vieira ◽  
D Knobeloch ◽  
A Nüssler ◽  
...  
Keyword(s):  
Liver Cell ◽  
Human Liver ◽  
In Vitro Toxicity ◽  
Cell Cultivation ◽  
Toxicity Studies ◽  
Human Liver Cell ◽  
Bioreactor System
Sign in / Sign up

Export Citation Format

Share Document