scholarly journals Increasing Stock Production of Two Herbaceous Perennials with the Application of Plant Growth Regulators

2020 ◽  
Vol 30 (3) ◽  
pp. 421-427
Author(s):  
Sean J. Markovic ◽  
James E. Klett

The objective of these experiments was to evaluate the reaction of ‘Snow Angel’ coral bells (Heuchera sanguinea) and Orange Carpet hummingbird trumpet (Epilobium canum ssp. garrettii ‘PWWG01S’) to repeated foliar applications of three plant growth regulators at two application rates. The plant growth regulators applied during a stock plant study and followed by a propagation study were 200 and 400 ppm ethephon, 250 and 500 ppm benzyladenine, and 50 and 100 ppm gibberellic acid 4 and 7 (GA4+7) + benzyladenine. The stock plant study was conducted to assess the efficacy of plant growth regulators, vegetative growth (height and width growth index), the number of vegetative cuttings, as well as the fresh weight (FW) and dry weight (DW) of the harvested vegetative cuttings. The propagation study was conducted to determine the effects of the plant growth regulator treatments on the rooting of the vegetative cuttings. The stock plant study showed that GA4+7 + benzyladenine (50 and 100 ppm) significantly increased production of ‘Snow Angel’ coral bells cuttings compared with all other treatments. However, no significant differences in FW or DW were observed with ‘Snow Angel’ coral bells between treatments. In the propagation study, no significant difference in rooting percentage was observed after 4 weeks. The Orange Carpet hummingbird trumpet stock plant study resulted in a greater number of vegetative cuttings with GA4+7 + benzyladenine (50 and 100 ppm) and benzyladenine (250 ppm) treatments. Fresh weight of vegetative cuttings harvested from plants treated with GA4+7 + benzyladenine (50 or 100 ppm) were the lowest. The only treatment that showed increased vegetative cutting production with no effect on FW was benzyladenine (250 ppm) on Orange Carpet hummingbird trumpet.

2021 ◽  
pp. 1-7
Author(s):  
Sean J. Markovic ◽  
James E. Klett

This study aimed to assess the effects of plant growth regulators (PGRs) on stock plant production of mojave sage (Salvia pachyphylla) and ‘Avalanche’ cape daisy (Osteospermum hybrid) that received foliar sprays of the following three PGRs: 200 and 400 ppm ethephon; 250 and 500 ppm benzyladenine; and 50 and 100 ppm gibberellic acid 4 and 7 (GA4+7) plus benzyladenine. Vegetative growth [height and width growth index (GI)], the number of vegetative cuttings, and fresh weight (FW) and dry weight (DW) of the harvested vegetative cuttings data were collected. A propagation study was conducted concurrently to determine the effects of the PGR treatments on rooting vegetative cuttings. GA4+7 plus benzyladenine (50 and 100 ppm) increased the production of both mojave sage and ‘Avalanche’ cape daisy cuttings by ≥18% more than the other treatments. The GI, FW, and DW results showed similar trends across experiments 1 and 2 for each perennial. In the propagation study, the rooting percentage did not differ after 4 weeks, indicating that the use of GA4+7 plus benzyladenine in production protocols could benefit producers of both perennials.


2021 ◽  
Vol 39 (2) ◽  
pp. 62-67
Author(s):  
Sean J. Markovic ◽  
James E. Klett

Abstract Moroccan pincushion (Pterocephalus depressus) is a drought-tolerant perennial that is being used in landscapes throughout arid areas of the western United States. This paper describes two experiments researching vegetative cutting production from stock plants. Moroccan pincushion stock plants received foliar applications of gibberellic acid (GA3), benzyladenine, ethephon, or auxin [indole-3-butyric acid (IBA)] plant growth regulators (PGR). Plant growth regulators were applied singularly and in combination with GA3 to determine efficacy on stock plant growth. A propagation study was conducted simultaneously to determine effects of these different PGR treatments applied to stock plants on the rooting of moroccan pincushion cuttings. The stock plant study showed GA3 + benzyladenine application increased cutting production over other PGR treatments. Fresh weight of moroccan pincushion cuttings did not differ among treatments. While cuttings did not differ in dry weight in experiment 1, statistical differences were observed in experiment 2. However, these differences in dry weight did not affect the quality of the cuttings. Cuttings from stock plants treated with GA3 + IBA treatment had the highest numerical growth index [(height + width + width)/3]. Cuttings from stock plants treated with GA3 alone or in combination with another PGR were all greater in average growth index and statistically differed from those without GA3 being applied. PGR treatments did not affect rooting percentages of the cuttings with nontreated stock plant cuttings successfully rooting at an average rate of 95%. However, GA3 + IBA was the only treatment where cuttings had 100% rooting for both experiments, indicating potential rooting benefits. Index words: Plant growth regulator, propagation, Pterocephalus depressus, vegetative cuttings. Species used in this study: Moroccan pincushion [Pterocephalus depressus Archibald]. Chemicals used in this study: gibberellic acid (GA3), benzyladenine, ethephon, indole-3-butyric acid (IBA).


HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 852D-852
Author(s):  
Sabrina L. Shaw ◽  
William F. Hayslett ◽  
Eddie B. Williams

A one-time application of fish emulsion 2 days before the application of plant growth regulators (PGR) showed an overriding effect on the growth of pansies. Blue/blotch shades of `Medallion' pansies were placed on a constant feed program of 100 ppm Peat Lite 20N–10P–20K, with half of the pansies receiving an additional one-time supplement of fish emulsion. PGRs and rates included B-Nine, 0.5% (used as the control); uniconazole, 2 and 4 ppm; and paclobutrazol, 16 and 25 ppm. Parameters taken included plant height, top fresh weight, top dry weight, days to anthesis, and visual appearance. Significant differences were noted in the plants receiving the supplement for plant dry weight, plant height, and visual appearance. Plants receiving fish emulsion grew taller and denser than those on constant feed alone despite the effects of the PGRs.


2017 ◽  
Vol 48 (5) ◽  
Author(s):  
Al-Obaidy & Khierallah

This research was conducted to study the effect of some plant growth regulators on in vitro shoots multiplication of stevia (Stevia rebaudiana Bertoni). The experiments included tests of various combinations of KIN with IBA or IAA in the shoot multiplication. Results indicated that KIN at 1.0 mg. L-1 plus 0.3 mg. L-1 of IBA produced the highest number of shoots (3.5 shoots) while KIN at 1.5 mg. L-1 plus IBA at 1.0 mg. L-1 produced the lowest shoot length (1.14 cm).  Hormone free medium produced the highest rate of the leaves number reached 28.56 leaves. KIN and IBA interaction increased fresh and dry weight significantly.   Treatment contained 2.0 mg -1 KIN plus 0.3 mg. L-1 IBA produced the highest fresh weight (1.739 g) while 0.5 mg. L-1 KIN and 0.3 mg. L-1 IBA produced the highest dry weight (0.822 g). As for the effect of interaction between the IAA and KIN it was significant in the number of shoots formed. Interaction between 1.0 mg. L-1 KIN with 0.1 mg. L-1IAA produced the highest number of shoots (3.8 shoots). Shoots length reached 8.10 cm in the media with 0.3 mg. L-1 IAA only. The highest fresh weight (1.267 g) was achieved with the interaction between 1.0 mg. L-1 KIN and 0.3 mg. L-1 IAA while 0.5 mg. L-1IAA without KIN produced the highest dry weight reached 0.138 g.  Shoots multiplication was improved by incorporation of the cytokinin TDZ in culture media. Shoots number, fresh and dry weights were increased significantly by adding 0.05 mg. L-1 of TDZ at present of 0.3 mg. L-1 of IBA giving 6.6 shoots, 0.974 g and 0.144 g respectively while shoots length decreased significantly as media without TDZ produced the highest shoots length reached 9.32 cm. The above results can adopt for the successful in vitro shoot multiplication of Stevia plants. 


Author(s):  
Dwi Kusuma Wahyuni ◽  
Putri Andriani ◽  
Arif Nur Muhammad Ansori ◽  
Edy Setiti Wida Utami

<p class="IsiAbstrakIndo"><em>Justicia gendarussa </em>Burm.f., a medicinal plant, is Acanthaceae that has many functions. Furthermore, the compounds in gendarussa must be produced in high quantity and quality by applying callus culture method. Accordingly, it is important to study the effects of plant growth regulators (2,4-D, IBA, and BAP) on callus induction of gendarussa leaves. This research design utilized a factorial design with two factors (2,4-D and IBA: 0.5, 1, 1.5 mg/L and BAP: 0.5, 1, 1.5, 2 mg/L). The experiment consisted of 24 treatments, each of which was repeated 3 times. Observation was carried out in 6 weeks. Data on the time of callus formation, percentage of explants formed callus, and callus morphology were analyzed descriptively,while data on fresh and dry weight were analyzed by Two-Way ANOVA (<span>α</span> = 0.5). Interestingly, the results showed that various concentration of plant growth regulators (2,4-D, IBA, and BAP) affected callus induction from leaf explants of gendarussa. We concluded that the most optimal treatment combination of concentration of plant growth regulators in inducing callus from leaf explants of gendarussa is 1.5 mg/L 2,4-D and 2 mg/L BAP with a relatively long period of callus formation at the earliest, i.e. on day 5, 2.247 g of fresh weight, 0.108 gof dry weight, white callus translucent, and friable. <span lang="EN-GB">Moreover, t</span>he optimum treatment will be used to produce secondary metabolite and seed s<span lang="EN-GB">y</span>nthetic by cell suspension culture.</p>


2016 ◽  
Vol 19 (3) ◽  
pp. 43-51
Author(s):  
Bup Kim Nguyen ◽  
Tien Thi Thuy Le ◽  
Viet Trang Bui

The growth of Ta Xanh coconut fruit is divided into four stages: slow growth, rapid growth, mature and ripening. In the mature and ripening stages, lipid accumulation increased at the strongest level in the endosperm. This study was conducted with the purpose of studying the effects of plant growth regulators on the growth and lipid accumulation in copra (solid endosperm) from the mature to ripening stage in Ta Xanh coconut fruit. Fresh weight, dry weight and lipid content in solid endosperm after treatments with 1 mg/L NAA, 10 mg/L BA and 20 mg/L GA3 individually or in combination with ethrel were determined. Results showed that the treatment of 20 mg/L GA3 on the 8-month-old fruits increased the copra fresh weight and dry weight. Treatments of 1 mg/L NAA, 20 mg/l GA3 or 200-250 mg/L ethrel on the 10-month-old fruits increased the ratio of lipid in the copra of each fruit. Treatment of 200 mg/L ethrel had a positive effect on increasing of the fresh weight, dry weight and lipid concent in the solid endosperm of the 10-month-old Ta Xanh coconut after 1 or 2 weeks of combinated treatment of 1 mg/L NAA, 10 mg/L BA and 20 mg/L GA3.


2017 ◽  
Vol 14 (3) ◽  
pp. 461-468
Author(s):  
Baghdad Science Journal

Ricinus communis L. is an important medical plant hence it contains many active compounds. The aim of this research is to study the effect of plant growth regulators on callus induction and Rutin concentration. A combination of Benzyle adenine (BA) and Indol Acetic acid (IAA) at (0.0,1.0,2.0) mg/L was added to the media, the highest fresh weight of the induced callus from stem explant was (4.97) gr . at (1.0,1.0) mg/L BA and IAA consenquently the same combination gave the highest dry weight of callus (0.42) gr. while the combination at (2.0,1.0) mg/L BA and IAA gave the highest fresh weight of induced callus from Leaves explant (5.28) gr., then (2.0,1.0) mg/L BA and IAA gave the highest dry weight for callus induced from leaves at (0.55)gr.Spectrophotometer used to estimate rutin quantity and results showed that the present of rutin at (126.31) ppm in callus induced from stem at (2.0,2.0) mg/L BA and IAA, the highest value of this compound (121.05) ppm on callus induction from the leaves in the same combination as compared with Rutin quantity in intact plant that reached (94.73) ppm in leaves and (68.42) ppm in stem.


EUGENIA ◽  
2012 ◽  
Vol 21 (3) ◽  
Author(s):  
Stanley A.F. Walingkas ◽  
Meity Rantung

ABSTRACT This study aims to determine the effect of plant growth regulators and micro elements to cherelle wilt in cacao plants as well as finding the right business in tackling cherelle wilt in cacao plants. The research was carried out in the garden village folk Eris Eris Minahasa district for 8 months from January to August 2009. Spraying micro-nutrient elements is necessary to reduce cherelle wilt because the various treatments for the first observation is not significantly different, but the observations into two to seven observations to indicate a significant difference between treatments (percentage cherelle wilt on treatment without nutrient elements higher than sprayed). While spraying the treatment factor of plant growth regulators (NAA) is not necessary because the first observation and the two had no significant effect on the percentage of cherelle wilt affected only the third observation and further observations to a fourth, fifth, sixth and seventh again no effect. So is the treatment for fruit length and diameter showed that administration of the micro nutrients necessary for sprayed with micro nutrients result 22.10 cm higher than that is not sprayed is 19.43 cm. As for the diameter of the fruit is affected by plant growth regulators. Treatment is better than 200 ppm 100 ppm and 0 ppm (control). Keywords: NAA, cocoa, mikombi     ABSTRAK   Penelitian ini bertujuan mengetahui pengaruh pemberian zat pengatur tumbuh dan unsur mikro terhadap cherelle wilt pada tanaman kakao serta menemukan usaha yang tepat dalam menanggulangi cherelle wilt pada tanaman kakao. Penelitian ini dilaksanakan di kebun rakyat Desa Eris Kecamatan Eris Kabupaten Minahasa selama 8 bulan sejak Januari – Agustus 2009. Penyemprotan unsur hara mikro perlu dilakukan untuk mengurangi cherelle wilt sebab pada berbagai perlakuan untuk pengamatan pertama tidak berbeda nyata namun pada pengamatan ke dua sampai pengamatan ke tujuh menunjukkan adanya perbedaan nyata antar perlakuan (prosentase cherelle wilt pada perlakuan tanpa unsure hara lebih tinggi dari yang disemprot). Sementara faktor penyemprotan dengan perlakuan zat pengatur tumbuh (NAA) tidak perlu dilakukan sebab pengamatan pertama dan ke dua tidak berpengaruh nyata pada persentase cherelle wilt hanya berpengaruh pada pengamatan ke tiga dan selanjutnya pengamatan ke empat, ke lima, ke enam dan ke tujuh kembali tidak berpengaruh. Begitu juga perlakuan untuk panjang dan diameter buah menunjukkan bahwa pemberian unsur hara mikro perlu dilakukan sebab yang disemprot dengan unsur hara mikro hasilnya 22,10 cm lebih tinggi dari yang tidak disemprot yaitu 19,43 cm. Sementara untuk diameter buah dipengaruhi oleh zat pengatur tumbuh. Perlakuan 200 ppm lebih baik dari 100 ppm dan 0 ppm (kontrol). Eugenia Volume 18 No. 2  Agustus 2012 Kata kunci : NAA, kakao, mikombi


Author(s):  
R. Sivakumar

Background: Horsegram is an important pulse crop grown as Rabi crop in Tamil Nadu. Kharif sown crop could not flowers may be because of more number of tendrils produced as compare to Rabi sown crop. Hence, an attempt was made to reduce the number of tendrils and induce flowering in horsegram during Kharif season by plant growth regulators. Methods: A field experiment was conducted to study the impact of plant growth regulators viz., salicylic acid (100 ppm), chlormequat chloride (CCC - 250 ppm), mepiquat chloride (250 ppm), tri iodo benzoic acid (TIBA - 200 ppm) and nitrobenzene (0.2%) on physiological traits and tendril growth associated with flowering in horsegram during Kharif. Different treatments were applied through foliar application at 25 Days after sowing. Result: Among the PGRs, foliar application of TIBA registered the lowest number of tendrils (2.3) followed by CCC (3.0) compared to other treatments. Higher root length of 16.8 cm and RWC of 82.3% was found in CCC treatment. CCC also registered the highest photosynthetic rate (27.15 µmol m-2 s-1), transpiration rate (18.06 mmol m-2 s-1) and lowest leaf temperature (26°C) compared to other treatments. The highest soluble protein content of 13.1 mg g-1 was also estimated in CCC treatment followed by mepiquat chloride (12.51 mg g-1). Sucrose phosphate synthase (SPS) activity did not show any significant difference between the treatments. Number of flowers buds formed per plant was zero and hence the flowering did not take place in any treatments. Among the plant growth regulators used, TIBA and CCC registered its positive action on reduced the number of tendrils and leaf temperature, but not enough to induce flowering under Kharif season.


Weed Science ◽  
1975 ◽  
Vol 23 (5) ◽  
pp. 428-432
Author(s):  
T. J. Allen ◽  
C. L. Leinweber ◽  
D. K. Prince ◽  
D. F. Bouchard

CBC (2,5-dichlorobenzoyl chloride) and MCB (methyl-2,5-dichlorobenzoate) function as plant growth regulators in a gaseous phase or in an aqueous solution. Sorghum [Sorghum bicolor (L.) Moench. ‘Martin’] and soybean [Glycine bicolor (L.) Merr. ‘Lee’] seeds were temporarily inhibited or delayed from germinating, but overcame the inhibition in time so there was no significant reduction in total germination. Seedlings produced from sorghum and soybean seed germinated and grown in aqueous solution of 35 μg/ml CBC or MCB were shorter and weighed less than control seedlings. Soybean seed treated with the vapors of CBC and MCB produced seedlings significantly reduced in dry weight as compared with that of controls. Foliar application of 500 or 1000 μg/ml of CBC significantly reduced the growth of soybean but not sorghum seedlings. Soybean seedlings treated with the vapors or an aqueous solution produced leaflets more narrow and thicker than control seedlings. A lethal response attributable to CBC or MCB was not recorded.


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