scholarly journals Comparison of the Phenolic Profiles of Juice and Cider Derived from Machine- and Hand-harvested ‘Brown Snout’ Specialty Cider Apples in Northwest Washington

2019 ◽  
Vol 29 (4) ◽  
pp. 423-433
Author(s):  
Travis R. Alexander ◽  
Thomas S. Collins ◽  
Carol A. Miles
Keyword(s):  
High Performance ◽  
Lower Cost ◽  
Labor Costs ◽  
Redox Titration ◽  
Phenolic Profile ◽  
Flight Mass Spectrometry ◽  
Phenolic Profiles ◽  
Titration Assay ◽  
Harvest Method ◽  
Similar Yield

‘Brown Snout’ cider apple (Malus ×domestica) is desired by cider makers for its relatively high levels of phenolics, and over-the-row machine harvesting of ‘Brown Snout’ has been demonstrated to provide similar yield to hand harvest at a significantly lower cost. The purpose of this study was to determine if there is a measurable impact of harvest method on the phenolic profile of ‘Brown Snout’ juice and cider to better inform equipment adoption recommendations. Using a redox titration assay, the titratable tannin content (± SE) of juice (0.19% ± 0.01%) and cider (0.19% ± 0.01%) were found not to differ due to harvest method. Using a protein precipitation assay, juice from machine-harvested fruit was found to have lower levels of total tannins [231 ± 36 mg·L−1 catechin equivalents (CE)] than juice from hand-harvested fruit (420 ± 14 mg·L−1 CE). However, the total tannins of cider did not differ due to harvest method, the overall average for machine and hand harvest was 203 ± 22 mg·L−1 CE. The total phenolics of juice and cider did not differ due to harvest method (1415 ± 98 mg·L−1 CE and 1431 ± 73 mg·L−1 CE, respectively). Discriminant analysis based on an average of 33 tentatively identified phenolic compounds, as measured by ultra-high performance liquid chromatography coupled with quadrupole time of flight mass spectrometry, showed no separation due to harvest method in juice or cider. In conclusion, over-the-row machine harvesting of ‘Brown Snout’ resulted in a final product of similar quality at reduced labor costs, and thus shows potential for increasing the commercial sustainability of cider apple operations.

New insights into the qualitative phenolic profile of Ficus carica L. fruits and leaves from Tunisia using ultra-high-performance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry and their antioxidant activity

RSC Advances ◽  
2015 ◽  
Vol 5 (26) ◽  
pp. 20035-20050 ◽  
Author(s):  
Sonda Ammar ◽  
María del Mar Contreras ◽  
Olfa Belguith-Hadrich ◽  
Mohamed Bouaziz ◽  
Antonio Segura-Carretero
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Ficus Carica ◽  
Phenolic Profile ◽  
Flight Mass Spectrometry ◽  
Phenolic Constituents ◽  
Antioxidant Potency

RP-UHPLC-DAD-QTOF-MS gives new insights into the fig phenolic constituents that correlate with their antioxidant potency.

scholarly journals Comparison of the Phenolic Profiles of Soaked and Germinated Peanut Cultivars via UPLC-QTOF-MS

Antioxidants ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 47 ◽  
Author(s):  
Qiong-Qiong Yang ◽  
Lin Cheng ◽  
Zhi-Yuan Long ◽  
Hua-Bin Li ◽  
Anil Gunaratne ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Antioxidant Activities ◽  
Natural Antioxidants ◽  
Total Phenolic ◽  
Human Consumption ◽  
Flavonoid Content ◽  
Flight Mass Spectrometry ◽  
Phenolic Profiles ◽  
Peanut Cultivars

Diverse peanut varieties are widely cultivated in China. However, few studies have investigated the effects of germination on the phenolic profiles and antioxidant activities of specific Chinese peanut cultivars. Therefore, this study was conducted to evaluate the effects of germination on total phenolic content (TPC), total flavonoid content (TFC), antioxidant activity, and phenolic profiles of seven peanut cultivars in China. The TPC, TFC, and antioxidant activities were determined by spectrophotometry, while phenolic profiles were analyzed by using ultra-high performance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UPLC-QTOF-MS). The results found that germination significantly increased TPC, TFC, and antioxidant activity. Antioxidant activity was found to be closely related to TPC in germinated peanut extracts, which indicates that phenolics are the main contributors of antioxidants in germinated peanuts. In addition, germination induced significant changes in polyphenolic profiles. In the analyzed samples, 36 phenolic compounds were identified in which most were flavonoids. Overall, these findings highlight that germinated peanuts can be a good natural source of natural antioxidants for human consumption and functional food development.

scholarly journals Uptake and bio-transformation of telmisartan by cress (Lepidium sativum) from sewage treatment plant effluents using high-performance liquid chromatography/drift-tube ion-mobility quadrupole time-of-flight mass spectrometry

2021 ◽  
Author(s):  
Tamara Lang ◽  
Markus Himmelsbach ◽  
Franz Mlynek ◽  
Wolfgang Buchberger ◽  
Christian W. Klampfl
Keyword(s):  
High Performance ◽  
Waste Water Treatment ◽  
Sewage Treatment ◽  
Sewage Treatment Plant ◽  
Treatment Plant ◽  
Parent Drug ◽  
Water Treatment Plant ◽  
Lepidium Sativum ◽  
Waste Water Treatment Plant ◽  
Flight Mass Spectrometry

AbstractIn the present study, the uptake and metabolization of the sartan drug telmisartan by a series of plants was investigated. Thereby for seven potential metabolites, modifications on the telmisartan molecule such as hydroxylation and/or glycosylation could be tentatively identified. For two additional signals detected at accurate masses m/z 777.3107 and m/z 793.3096, no suggestions for molecular formulas could be made. Further investigations employing garden cress (Lepidium sativum) as a model plant were conducted. This was done in order to develop an analytical method allowing the detection of these substances also under environmentally relevant conditions. For this reason, the knowledge achieved from treatment of the plants with rather high concentrations of the parent drug (10 mg L−1) was compared with results obtained when using solutions containing telmisartan in the μg - ng L−1 range. Thereby the parent drug and up to three tentative drug-related metabolites could still be detected. Finally cress was cultivated in water taken from a local waste water treatment plant effluent containing 90 ng L−1 of telmisartan and harvested and the cress roots were extracted. In this extract, next to the parent drug one major metabolite, namely telmisartan-glucose could be identified.

scholarly journals Metabolomics and DNA-Based Authentication of Two Traditional Asian Medicinal and Aromatic Species of Salvia subg. Perovskia

Cells ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 112
Author(s):  
Monika Bielecka ◽  
Bartosz Pencakowski ◽  
Marta Stafiniak ◽  
Klemens Jakubowski ◽  
Mehdi Rahimmalek ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
High Performance ◽  
Carnosic Acid ◽  
Limited Information ◽  
Metabolomic Analysis ◽  
Separate Species ◽  
Flight Mass Spectrometry ◽  
Aerial Parts ◽  
Central Asian ◽  
Aromatic Species

Subgenus Perovskia of the extended genus of Salvia comprises several Central Asian medicinal and aromatic species, of which S. yangii and S. abrotanoides are the most widespread. These plants are cultivated in Europe as robust ornamentals, and several cultivars are available. However, their medicinal potential remains underutilized because of limited information about their phytochemical and genetic diversity. Thus, we combined an ultra-high performance liquid chromatography quadrupole time of flight mass spectrometry (UHPLC-QTOF-MS) based metabolomics with DNA barcoding approach based on trnH-psbA and ITS2 barcodes to clarify the relationships between these two taxa. Metabolomic analysis demonstrated that aerial parts are more similar than roots and none of the major compounds stand out as distinct. Sugiol in S. yangii leaves and carnosic acid quinone in S. abrotanoides were mostly responsible for their chemical differentiation, whereas in roots the distinction was supported by the presence of five norditerpenoids in S. yangii and two flavonoids and one norditerpenoid in S. abrotanoides. To verify the metabolomics-based differentiation, we performed DNA authentication that revealed S. yangii and S. abrotanoides to be very closely related but separate species. We demonstrated that DNA barcoding coupled with parallel LC-MS profiling constitutes a powerful tool in identification of taxonomically close Salvia species.

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