scholarly journals Sample Container and Storage Temperature for Paclobutrazol Monitoring in Irrigation Water

2015 ◽  
Vol 25 (6) ◽  
pp. 769-773 ◽  
Author(s):  
James E. Altland ◽  
Leslie Morris ◽  
Jennifer Boldt ◽  
Paul Fisher ◽  
Rosa Raudales

Paclobutrazol is a plant growth retardant commonly used on greenhouse crops. Residues from paclobutrazol applications can accumulate in recirculated irrigation water. Given that paclobutrazol has a long half-life and potential biological activity in parts per billion concentrations, it would be desirable to measure paclobutrazol concentration in captured irrigation supplies. However, there are no standard protocols for collecting this type of sample. The objective of this research was to determine if sample container material or storage temperature affect paclobutrazol stability over time. In two experiments, paclobutrazol was mixed in concentrations ranging from 0.04 to 0.2 mg·L−1 and stored in polyethylene, clear glass, or amber glass containers at temperatures of either 4 or 20 °C. Paclobutrazol concentration was measured at 3, 14, and 30 days after the start of each experiment. Across the two experiments, there were no consistent trends in reduction of paclobutrazol concentration with respect to container material or storage temperature. In the first experiment, there was an average of 5% reduction across all treatments from day 0 to 30, whereas in the second experiment, concentration did not decrease over the 30-day time period. These data suggest that paclobutrazol is stable in collected water samples for at least 30 days, and that either glass or polyethylene containers are suitable for collecting greenhouse water samples for analysis of paclobutrazol concentration. A minimum volume of 100 mL was determined to be the optimum to analyze water samples with diverse paclobutrazol concentrations.

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
AJAY KUMAR RAJAWAT ◽  
PRAVEEN KUMAR

An attempt has been made to study the Physico-chemical condition of water of Yamuna River at Gokul Barrage, Mathura, (UP). The time period of study was July 2015 to June 2016. Three water samples were selected from different sites in each month for study. The parameters studied were Temperature, Turbidity, pH, DO, BOD, COD, Total Dissolved Solids and Suspended Solids. Almost all the parameters were found above the tolerance limit.


Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2339
Author(s):  
So-Yul Yun ◽  
Jee-Young Imm

Age gelation is a major quality defect in ultra-high-temperature (UHT) pasteurized milk during extended storage. Changes in plasmin (PL)-induced sedimentation were investigated during storage (23 °C and 37 °C, four weeks) of UHT skim milk treated with PL (2.5, 10, and 15 U/L). The increase in particle size and broadening of the particle size distribution of samples during storage were dependent on the PL concentration, storage period, and storage temperature. Sediment analysis indicated that elevated storage temperature accelerated protein sedimentation. The initial PL concentration was positively correlated with the amount of protein sediment in samples stored at 23 °C for four weeks (r = 0.615; p < 0.01), whereas this correlation was negative in samples stored at 37 °C for the same time (r = −0.358; p < 0.01) due to extensive proteolysis. SDS-PAGE revealed that whey proteins remained soluble over storage at 23 °C for four weeks, but they mostly disappeared from the soluble phase of PL-added samples after two weeks’ storage at 37 °C. Transmission electron micrographs of PL-containing UHT skim milk during storage at different temperatures supported the trend of sediment analysis well. Based on the Fourier transform infrared spectra of UHT skim milk stored at 23 °C for three weeks, PL-induced particle size enlargement was due to protein aggregation and the formation of intermolecular β-sheet structures, which contributed to casein destabilization, leading to sediment formation.


Agriculture ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 374
Author(s):  
Teresa Szczęsna ◽  
Ewa Waś ◽  
Piotr Semkiw ◽  
Piotr Skubida ◽  
Katarzyna Jaśkiewicz ◽  
...  

The aim of this study was to determine the influence of storage temperature and time on physicochemical parameters of starch syrups recommended for the winter feeding of bee colonies. The studies included commercially available three starch syrups and an inverted saccharose syrup that were stored at different temperatures: ca. 20 °C, 10–14 °C, and ca. 4 °C. Physicochemical parameters of fresh syrups (immediately after purchase) and syrups after 3, 6, 9, 12, 15, 18, 21, and 24 months of storage at the abovementioned temperatures were measured. It was observed that the rate of unfavorable changes in chemical composition of starch syrups and the inverted saccharose syrup, mainly the changes in the 5-hydroxymethylfurfural (HMF) content, depended on the type of a syrup and storage conditions (temperature, time). Properties of tested starch syrups intended for winter feeding of bees stored at ca. 20 °C maintained unchanged for up to 6 months, whereas the same syrups stored at lower temperatures (10–14 °C) maintained unchanged physicochemical parameters for about 12 months. In higher temperatures, the HMF content increased. To date, the influence of this compound on bees has not been thoroughly investigated.


Catalysts ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 527
Author(s):  
Katarzyna Czyzewska ◽  
Anna Trusek

The current requirements of industrial biocatalysis are related to economically beneficial and environmentally friendly processes. Such a strategy engages low-temperature reactions. The presented approach is essential, especially in food processes, where temperature affects the quality and nutritional value foodstuffs. The subject of the study is the hydrolysis of lactose with the commercial lactase NOLA™ Fit 5500 (NOLA). The complete decomposition of lactose into two monosaccharides gives a sweeter product, recommended for lactose intolerant people and those controlling a product’s caloric content. The hydrolysis reaction was performed at 15 °C, which is related to milk transportation and storage temperature. The enzyme showed activity over the entire range of substrate concentrations (up to 55 g/L lactose). For reusability and easy isolation, the enzyme was encapsulated in a sodium alginate network. Its stability allows carrying out six cycles of the complete hydrolysis of lactose to monosaccharides, lasting from two to four hours. During the study, the kinetic description of native and encapsulated NOLA was conducted. As a result, the model of competitive galactose inhibition and glucose mixed influence (competitive inhibition and activation) was proposed. The capsule size does not influence the reaction rate; thus, the substrate diffusion into capsules can be omitted from the process description. The prepared 4 mm capsules are easy to separate between cycles, e.g., using sieves.


2020 ◽  
Vol 29 (10) ◽  
pp. 1433-1438
Author(s):  
Ho-Seok Chang ◽  
Kwang-Young Song ◽  
Young-Ji Kim ◽  
Jung-Whan Chon ◽  
Hyunsook Kim ◽  
...  

2017 ◽  
Vol 229 ◽  
pp. 610-620 ◽  
Author(s):  
Laura T. Rodriguez Furlán ◽  
Yanina Baracco ◽  
Javier Lecot ◽  
Noemi Zaritzky ◽  
Mercedes E. Campderrós

2005 ◽  
Vol 36 (12) ◽  
pp. 1197-1206 ◽  
Author(s):  
Lars Helge Stien ◽  
Elisabeth Hirmas ◽  
Marit Bjornevik ◽  
Orjan Karlsen ◽  
Ragnar Nortvedt ◽  
...  

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