scholarly journals Winter Stem Cutting Propagation of Heller’s Japanese Holly with and without Use of a Conventional Auxin Treatment

2012 ◽  
Vol 22 (6) ◽  
pp. 771-773 ◽  
Author(s):  
Eugene K. Blythe ◽  
Jeff L. Sibley
Keyword(s):  
Slow Growth ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Department Of Agriculture ◽  
Slow Growth Rate ◽  
Cutting Propagation ◽  
Auxin Treatment ◽  
Landscape Plant ◽  
Dark Green ◽  
High Humidity Environment

Heller’s japanese holly [Ilex crenata ‘Helleri’ (synonym: Ilex crenata f. helleri)] is a popular landscape plant in U.S. Department of Agriculture hardiness zones 5b to 8a because of its dwarf habit, slow growth rate, and dark green leaves. Plants can be propagated readily by stem cuttings and use of an auxin treatment is generally recommended to promote rooting. This study was conducted to determine if auxin treatment could be eliminated, thus reducing labor and chemical requirements in the cutting propagation process. In three experiments, terminal stem cuttings of Heller’s japanese holly were taken in winter, prepared both with and without use of a basal quick-dip in an auxin solution [2500 ppm indole-3-butyric acid (IBA) + 1250 ppm 1-naphthaleneacetic acid (NAA)], and rooted in a warm, high-humidity environment. Both nontreated cuttings and cuttings receiving a 1-second basal quick-dip in the auxin solution rooted at, or near, 100%. However, treatment of cuttings with auxin resulted in larger root systems on the rooted cuttings, which could allow earlier transplanting into larger nursery containers. No inhibition of new spring growth was exhibited by cuttings treated with auxin in comparison with nontreated cuttings.

scholarly journals Hardwood Cutting Propagation of Confederate Rose Using Auxin and Wounding Treatments

2012 ◽  
Vol 22 (4) ◽  
pp. 476-478
Author(s):  
Eugene K. Blythe
Keyword(s):  
Root Length ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Total Root Length ◽  
Department Of Agriculture ◽  
Rooted Cuttings ◽  
Cutting Propagation ◽  
Nursery Production ◽  
Hardwood Cutting ◽  
High Humidity Environment

Confederate rose (Hibiscus mutabilis), a native of southeastern China, is an old-fashioned, ornamental plant often found in older gardens in the southern United States. Current breeding programs aim at developing selections with improved garden performance, thus providing new cultivars for nursery production. Hardy in U.S. Department of Agriculture (USDA) zones 7 to 9, plants grow as large shrubs or small trees in warmer areas, but generally die back to a woody base or short trunk in colder areas of their range. Stems from the past growing season that remain on plants during the winter in the warmer regions may be used to prepare hardwood stem cuttings. The current study examined hardwood cutting propagation of confederate rose in response to a 1-second basal quick-dip in auxin [1000 ppm indole-3-butyric acid (IBA), 3000 ppm IBA, 1000 ppm IBA + 500 ppm 1-naphthaleneacetic acid (NAA), and 3000 ppm IBA + 1500 ppm NAA] and a basal wound (along with 1000 ppm IBA only). Cuttings were rooted in a warm, high-humidity environment within a greenhouse. Auxin treatments improved overall rooting percentage and total root length, with 1000 ppm IBA (without and with a basal wound) providing the highest rooting percentages (about 70%) and nontreated cuttings the lowest (44%). A significant increase in total root length on rooted cuttings resulted with the use of 3000 ppm IBA (211 cm) and use of a basal wound plus 1000 ppm IBA (193 cm) compared with nontreated cuttings (87 cm). Auxin and wounding treatments did not have any significant inhibitory effects on budbreak and growth of new shoots on rooted cuttings.

scholarly journals Winter Stem Cutting Propagation of ‘Dwarf Burford’ Holly without Use of a Conventional Auxin Treatment

2009 ◽  
Vol 19 (1) ◽  
pp. 130-132 ◽  
Author(s):  
Eugene K. Blythe ◽  
Jeff L. Sibley
Keyword(s):  
Root Length ◽  
Stem Cutting ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Total Root Length ◽  
Growth Responses ◽  
Cutting Propagation ◽  
Auxin Treatment ◽  
Primary Roots ◽  
High Humidity Environment

‘Dwarf Burford’ holly (Ilex cornuta ‘Dwarf Burford’) is a significant nursery crop and is widely used in landscapes in U.S. Department of Agriculture hardiness zones 7 to 9. Stem cuttings can be rooted at multiple times during the year, provided cutting wood is sufficiently mature, with auxin treatments traditionally used to encourage rooting. This study was conducted to determine if auxin treatment could be eliminated, thus reducing labor and chemical requirements in the cutting propagation process. In three experiments, terminal stem cuttings of ‘Dwarf Burford’ holly were taken in winter, prepared with and without use of a basal quick-dip in an auxin solution, and rooted in a warm, high-humidity environment. Rooting percentages for nontreated cuttings and cuttings treated with 2500 ppm indole-3-butyric acid (IBA) + 1250 ppm 1-naphthaleneacetic acid (NAA) were similar, while treatment of cuttings with 5000 ppm IBA + 2500 ppm NAA resulted in a decrease in rooting percentage. The number of primary roots and total root length were similar among the three treatments, except in one experiment where total root length was greater with auxin-treated cuttings than with nontreated cuttings. Initial shoot growth responses were variable among the three experiments. The treatment of cuttings with auxin was not required for successful rooting and can be eliminated from the process for winter stem cutting propagation of ‘Dwarf Burford’ holly.

scholarly journals Rooting of ‘Brown Velvet’ Southern Magnolia Stem Cuttings as Influenced by Medium and Auxin Treatment

1996 ◽  
Vol 14 (3) ◽  
pp. 158-159
Author(s):  
T. Patrick McCracken ◽  
Christopher J. Catanzaro ◽  
Ted E. Bilderback
Keyword(s):  
Pine Bark ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Magnolia Grandiflora ◽  
Auxin Treatment ◽  
Primary Roots ◽  
Intermediate Response ◽  
Bottom Heat ◽  
Medium Selection ◽  
Secondary Roots

Abstract Terminal stem cuttings of ‘Brown Velvet’ Southern magnolia (Magnolia grandiflora L. ‘Brown Velvet’) were treated with 0.3% indole-3-butyric acid (IBA) in talc, or 0.5% naphthaleneacetic acid (NAA) quick dip + 0.3% IBA in talc, or 0.5% NAA + 1.0% IB A quick dip. Rooting media included pine bark, perlite, or combinations of bark and perlite at the following ratios: 3:1, 1:1, 1:3 (by vol). Cuttings were rooted in a greenhouse mist bed supplied with bottom heat. Rooting percentage was unaffected by media with the exception of a decreased response in perlite. Root length and secondary root formation was greater with pine bark than with perlite, while the bark/perlite blends provided an intermediate response. Increased auxin concentrations produced more primary roots but reduced formation of secondary roots. However, rooting percentage was not affected by auxin treatment. Results suggest that medium selection is more important than auxin source or delivery method in the rooting of ‘Brown Velvet’ Southern magnolia.

scholarly journals Responses to Propagation Substrate and Rooting Hormone Products to Facilitate Asexual Propagation of Silene chalcedonica and Silene coronaria

2016 ◽  
Vol 34 (3) ◽  
pp. 80-83
Author(s):  
Li Jiang ◽  
Bruce L. Dunn ◽  
Yun-wen Wang ◽  
Carla L. Goad
Keyword(s):  
Butyric Acid ◽  
Cost Effective ◽  
Adventitious Rooting ◽  
Stem Cuttings ◽  
Genetic Purity ◽  
Asexual Propagation ◽  
Cutting Propagation ◽  
Auxin Treatment ◽  
Napthaleneacetic Acid ◽  
Rooting Of Cuttings

Many ornamentals are commercially propagated by cuttings, which is an easy and cost-effective propagation method. Silene L. is a promising genus for extensive use as a landscape ornamental due to its drought tolerance, profuse flowering, and perennial characteristic. Silene is readily cross pollinated, so to ensure genetic purity for breeding and cultivar release, an asexual cutting propagation protocol needs to be established. Adventitious rooting of cuttings of Silene chalcedonica (L.) E.H.L. Krause and Silene coronaria (L.) Clairv. were evaluated using two different propagation substrates and three different root-promoting hormone products. Rooting percentage of S. chalcedonica cuttings was improved by Dip ‘N’ Grow treatment (1,000 mg·L−1), which contains 1% indole-3-butyric acid and 0.5% 1-napthaleneacetic acid, and Hormex Rooting Powder No. 1 (1,000 mg·L−1), which contains 0.10% indole-3-butyric acid; however, 89% of S. coronaria cuttings rooted readily in vermiculite without an auxin treatment. Therefore, rooting success of stem cuttings was highly species related. Because of the relatively low rooting percentage seen in vermiculite (46%) and in perlite (63%) using Dip ’N Grow auxin treatment for S. chalcedonica, an effective stem cutting propagation method still needs to be explored for this species.

Time-Energy Budgets During Reproduction and the Evolution of Single Parenting in the Superb Lyrebird

1986 ◽  
Vol 34 (3) ◽  
pp. 351 ◽  
Author(s):  
A Lill
Keyword(s):  
Growth Rate ◽  
Brood Size ◽  
Slow Growth ◽  
Bird Species ◽  
Egg Laying ◽  
Energy Budgets ◽  
Brood Care ◽  
Single Parenting ◽  
Slow Growth Rate ◽  
Small Brood

Estimated expenditures on brood-care by unassisted female superb lyrebirds, obtained through time-energy budgeting, were compared with published values for other bird species. With the exception of nestbuilding, estimated daily expenditures were relatively low, due mainly to the small brood size and low level of parental attentiveness. It is suggested that the traits which reduce daily brood-care expenditures, particularly the small brood size and extremely slow growth rate, may have evolved as adaptations which enabled deserted females operating close to maximal capacity to cope with single-parenting. Male parental involvement could probably increase the growth rate of the young, but not brood size; moreover, egg- laying was sufficiently asynchronous to afford multiple mating opportunities to parentally emancipated males. However, the slow growth rate of the young results in relatively large overall brood-care expenditures for females. Investments by males of up to 50% of daylight hours and 45% of BMR on singing at the height of the mating season were comparable with those of partially emancipated, polygynous males of other species. They probably reflect the high level of competition to control good display areas and to advertise status and quality to widely spaced females.

scholarly journals Methods of Auxin Application in Cutting Propagation: A Review of 70 Years of Scientific Discovery and Commercial Practice

2007 ◽  
Vol 25 (3) ◽  
pp. 166-185 ◽  
Author(s):  
Eugene K. Blythe ◽  
Jeff L. Sibley ◽  
Ken M. Tilt ◽  
John M. Ruter
Keyword(s):  
Scientific Discovery ◽  
Adventitious Rooting ◽  
Worker Safety ◽  
Stem Cuttings ◽  
Cutting Propagation ◽  
Commercial Practice ◽  
The Past ◽  
Application Techniques ◽  
Application Methods ◽  
Basic And Applied Research

Abstract The discovery of auxins as plant growth regulating chemicals in the 1930s and their ability to stimulate adventitious rooting in stem cuttings marked a major milestone in the modern history of plant propagation. Basic and applied research conducted during the past 70 years has provided information regarding absorption and translocation of auxin in cuttings, as well as the effects of timing of auxin application, methods of auxin application, type of auxin, and concentration of auxin on the rooting response of cuttings. The basal quick-dip method, the powder application method, and the dilute soak method have been the most commonly used methods for applying auxin to cuttings in commercial horticulture over the past 7 decades, with the first two methods still in common use today. In addition, a wide variety of other auxin application methods have been reported beginning in the 1930s and continuing to the present. Some of these methods remain primarily of historical interest, several find limited use in commercial horticulture, and others show potential for greater use in the future. Opportunities exist for further development of auxin application techniques that can enhance plant quality, efficiency and productivity of the cutting propagation process, and worker safety. This review provides horticulturists, from the academic researcher to the commercial propagator, with an exploration of auxin application methods over the past 70 years, along with an examination of the physiological response of cuttings to applied auxin and an overview of issues leading to future opportunities.

scholarly journals Effect of physiological age of stem and IBA treatment on rooting of branch cuttings of Taxus baccata L.

1970 ◽  
pp. 01-07
Author(s):  
Saumitro Das ◽  
L.K. Jha
Keyword(s):  
Growth Rate ◽  
Large Scale ◽  
Slow Growth ◽  
Physiological Age ◽  
Taxus Baccata ◽  
Practical Solution ◽  
Slow Growth Rate ◽  
Rooting Response ◽  
Himalayan Yew ◽  
Rooting Capacity

The natural population of Taxus baccata L. (Himalayan Yew) throughout the Indian Himalayan Region is greatly reduced due to its extensive and reckless exploitation for “Taxol” an anticancer drug. The effects of overexploitation are exacerbated by the species poor regeneration process, slow growth rate and prolonged seed dormancy. Therefore vegetative propagation by branch cuttings seems to be only practical solution for its large scale multiplication. A study was conducted on six candidate trees (CTs) to examine the effect genotype, physiological age of stem, IBA treatment on rooting of Taxus baccata cuttings. Results revealed that rooting behaviour of cuttings was significantly affected by all the factors under study. Among the six CTs studied, CT 2 (from BSI, Shillong) had given the highest rooting response (46.28%). The juvenile cuttings have the higher rooting capacity; however the callusing was more prominent in mature cutting. The influence of IBA treatment was also significant for rooting where 1000 was most effective for stimulating rooting juvenile cuttings and 2000 ppm in mature cuttings.

scholarly journals The mitochondrial AAA protease FTSH3 regulates Complex I abundance by promoting its disassembly

2021 ◽  
Author(s):  
Aneta Ivanova ◽  
Abi S Ghifari ◽  
Oliver Berkowitz ◽  
James Whelan ◽  
Monika W Murcha
Keyword(s):  
Complex I ◽  
Nadh Dehydrogenase ◽  
Slow Growth ◽  
Nadh:Ubiquinone Oxidoreductase ◽  
Temperature Sensitive ◽  
Wild Type ◽  
Forward Genetic Screen ◽  
Slow Growth Rate ◽  
Partial Restoration ◽  
Screen Approach

Abstract ATP is generated in mitochondria by oxidative phosphorylation. Complex I (NADH:ubiquinone oxidoreductase or NADH dehydrogenase) is the first multisubunit protein complex of this pathway, oxidising NADH and transferring electrons to the ubiquinone pool. Typically Complex I mutants display a slow growth rate compared to wild-type plants. Here, using a forward genetic screen approach for restored growth of a Complex I mutant, we have identified the mitochondrial ATP dependent metalloprotease, Filamentous Temperature Sensitive H 3 (FTSH3), as a factor that is required for the disassembly of Complex I. An ethyl methanesulfonate-induced mutation in FTSH3, named rmb1 (restoration of mitochondrial biogenesis 1), restored Complex I abundance and plant growth. Complementation could be achieved with FTSH3 lacking proteolytic activity, suggesting the unfoldase function of FTSH3 has a role in Complex I disassembly. The introduction of the rmb1 to an additional, independent, and extensively characterised Complex I mutant, ndufs4, resulted in similar increases to Complex I abundance and a partial restoration of growth. These results show that disassembly or degradation of Complex I plays a role in determining its steady-state abundance and thus turnover may vary under different conditions.

Ustilago bullata. [Descriptions of Fungi and Bacteria].

1981 ◽  
Author(s):  
J. E. M. Mordue
Keyword(s):  
Geographical Distribution ◽  
Latent Infection ◽  
Slow Growth ◽  
Poor Survival ◽  
Head Smut ◽  
Slow Growth Rate ◽  
North And South America ◽  
North And South ◽  
Leaf Distortion ◽  
Infection Hypha

Abstract A description is provided for Ustilago bullata. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: On Agropyron, Bromus, Brachypodium, Elymus, Festuca, Hordeum, Sitanion. DISEASE: Causes head smut of grasses particularly species of Bromus and Agropyron. Sori develop at the base of spikelets and usually involve the ovary but glumes are usually unaffected, phyllody of floral parts also occurs. Latent infection with very reduced sori production can also occur. Infected seedlings show stunting and poor survival, older plants show slow growth rate and leaf distortion has been observed (56, 265). GEOGRAPHICAL DISTRIBUTION: Europe, North and South America, W. Asia (USSR, Poland, Iraq), India, Kenya, Australia, New Zealand. TRANSMISSION: Teliospores are released from the inflorescence sori to contaminate soil and seed. Teliospores have remained viable (in artificial storage) for 12 years. Germination results in the production of a metabasidium and sporidia, plasmogamy then produces a dikaryotic infection hypha. Seedlings and older shoots become infected, the former producing completely infected plants, but the latter producing separately diseased tillers (see Falloon, 1979).

Embryonic oxygen consumption and growth of Laysan and black-footed albatross

1982 ◽  
Vol 242 (1) ◽  
pp. R121-R128 ◽  
Author(s):  
T. N. Pettit ◽  
G. S. Grant ◽  
G. C. Whittow ◽  
H. Rahn ◽  
C. V. Paganelli
Keyword(s):  
Oxygen Consumption ◽  
Slow Growth ◽  
Egg Mass ◽  
Low Oxygen ◽  
O2 Uptake ◽  
Prolonged Incubation ◽  
Slow Growth Rate ◽  
Air Space ◽  
Embryonic Metabolism ◽  
Pulmonary Respiration

The constraints placed on diffusive gas exchange by the eggshell and the adaptive features of embryonic respiration and metabolism in large Laysan and black-footed albatross eggs (300 g) during prolonged incubation (65 days) were examined in naturally incubated eggs on Sand Island, Midway, in the Northwestern Hawaiian Islands. A low eggshell gas conductance and slow growth rate were associated with a relatively low oxygen consumption (MO2) throughout incubation. Just prior to internal pipping (IP) of the inner shell membrane and penetration of the air space, the MO2 (pre-IP MO2) was approximately 1,250 ml O2 (STPD).day-1 for both species, resulting in air cell O2 and CO2 tensions of 106 and 40 Torr, respectively. During the 4- to 5-day pipping-to-hatching interval, O2 uptake increases rapidly as pulmonary respiration is initiated. Hatchling O2 consumption averaged 3,700 ml O2 (STPD).day-1 or about three times the pre-IP MO2. Data support the hypothesis that embryonic metabolism among Procellariiformes is related to the extent to which the incubation period deviates from the expected value based on initial egg mass.

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