scholarly journals Brief Exposure to Boiling Water Combined with Cold-moist Stratification Enhances Seed Germination of New Jersey Tea

2010 ◽  
Vol 20 (3) ◽  
pp. 623-625 ◽  
Author(s):  
J. Ryan Stewart ◽  
Irene McGary
Keyword(s):  
New Jersey ◽  
Sulfuric Acid ◽  
Seed Germination ◽  
Tallgrass Prairie ◽  
Germination Percentage ◽  
Boiling Water ◽  
High Quality ◽  
Natural Landscapes ◽  
Chilling Temperatures ◽  
Ceanothus Americanus

Although there is increasing interest in propagating prairie plants native to the midwestern United States for managed and natural landscapes, several species, including new jersey tea (Ceanothus americanus), are difficult to germinate from seeds. New jersey tea, which is an attractive, compact woody shrub, is found in high-quality prairie remnants throughout the tallgrass prairie region. Developing a protocol to increase the uniformity of seed germination in this species would allow for more widespread horticultural cultivation of this stress-resistant, nitrogen-fixing species. We hypothesized that the germination response of seeds of new jersey tea would be enhanced by replicating conditions that mimic their natural environment, which included treatments under controlled conditions exposing seeds to chilling temperatures, sulfuric acid, and boiling water. Two minutes of exposure to boiling water followed by 60 days of cold-moist stratification at 4 °C resulted in the highest germination percentage (48%) and mean daily germination (2.18 seeds/day). Scarification with 98% sulfuric acid for 15 min followed by 60 days of cold-moist stratification resulted in significant, but lower levels of germination percentage than seeds exposed to boiling water and cold-moist stratification. Cold-moist stratification in darkness and in an 18-hour photoperiod at 4 °C did not stimulate germination to a level suitable for production purposes. However, tetrazolium tests indicated that 79% of the seeds were viable. We conclude that cold-moist stratification should be used with boiling-water or acid-scarification to uniformly produce germinated seedlings of new jersey tea. However, nonresponsive seeds should not be discarded because they may germinate in later years if kept under appropriate conditions.

scholarly journals Dormancy break in seeds of "quina" (Strychnos pseudoquina A. St.-Hil.)

2011 ◽  
Vol 13 (4) ◽  
pp. 507-511 ◽  
Author(s):  
J.M. Vasconcelos ◽  
M.A. Rodrigues ◽  
S.C. Vasconcelos Filho ◽  
J.F. Sales ◽  
F.G. Silva ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Medicinal Plant ◽  
Gibberellic Acid ◽  
Plant Species ◽  
Germination Percentage ◽  
Boiling Water ◽  
Medicinal Plant Species ◽  
Distilled Water ◽  
Brazilian Cerrado ◽  
Germination Speed

"Quina" (Strychnos pseudoquina A. St. Hil) is a medicinal plant species from the Brazilian Cerrado. As its seeds show dormancy, they were subjected to the treatments pre-cooling at 5ºC during 7 days, pre-heating at 40ºC during 7 days, pre-soaking in sulfuric acid PA during 5 and 15 min, pre-soaking in boiling water during 5 and 15 min, pre-soaking in 100 and 200 ppm gibberellic acid during 48 h, pre-soaking in distilled water during 24 and 48 h, and mechanical scarification to break dormancy. Counts were daily conducted from the 2nd day after the experiment implementation until the germination stabilization at the 65th day. The germination speed index (GSI) and the germination percentage were evaluated. Germination rates above 96% were reached in seeds pre-soaked in water during 48 h and substrate moistened with water or KNO3.

scholarly journals Chemical Methods to Improve Seed Germination and Seedling Growth of Honey Locust (Gleditsia triacanthos L.)

2021 ◽  
Vol 78 (1) ◽  
pp. 63
Author(s):  
Liviu HOLONEC ◽  
Oana VIMAN ◽  
Steluta M. SÎNGEORZAN ◽  
Petru TRUȚA ◽  
Alina M. TRUȚA
Keyword(s):  
Sulfuric Acid ◽  
Seed Germination ◽  
Potassium Permanganate ◽  
Copper Sulphate ◽  
Germination Percentage ◽  
Physical Dormancy ◽  
Gleditsia Triacanthos ◽  
Chemical Treatments ◽  
Honey Locust ◽  
Concentrated Solution

The present study aimed to improve the seed germination and plantlet development of honey locust (Gleditsia triacanthos) using two types of edaphic substrate (peat and peat+sand) and different chemical substances. Breaking physical dormancy for some forest seeds is a challenge for researchers and forest managers in order to obtain a homogeneous germination on larger areas. Germination percentage of honey locust seeds were investigated by using different chemical treatments as follows: calcium hydroxide; concentrated solution of sodium 4-nitrophenolate; solution with a content of auxines; copper sulphate; potassium permanganate and concentrated sulfuric acid. The highest percentage of seed germination was obtained when the seeds were immersed in an aqueous solution of sulfuric acid (14%), followed by 12.5% (concentrated solution of sodium 4-nitrophenolate) and 12.3% (solution with a content of auxins). The lowest values (5%) were recorded when copper sulphate was used. Regarding the different types of studied substrates, the highest percentage of germination was obtained on peat + sand. As regards to seedling development, the highest values were obtained on the peat + sand substrate, with seeds treated with sulfuric acid (15.0 cm). The concentrated solution of sodium 4-nitrophenolate and solution with auxins recorded also high values, 15.2 cm, respectively 14.9 cm.  The lowest values for seedling height (11 cm) was noted when seeds were treated with potassium permanganate.

scholarly journals Effect of H2O-Based Low-Pressure Plasma (LPP) Treatment on the Germination of Bambara Groundnut Seeds

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 338
Author(s):  
Naeem Ahmed ◽  
Asad Masood ◽  
Kim S. Siow ◽  
M. F. Mohd Razip Wee ◽  
Rahmat Zaki Auliya ◽  
...  
Keyword(s):  
Food Security ◽  
Seed Germination ◽  
Chemical Modification ◽  
Low Pressure ◽  
Germination Percentage ◽  
Bambara Groundnut ◽  
Pressure Plasma ◽  
Low Pressure Plasma ◽  
Amine Groups

In general, seed germination is improved by low-pressure plasma (LPP) treatment using precursors such as air, nitrogen, argon, or water (H2O). Here, H2O-based LPP treatment using the optimized parameters of 10 W and 10 s improves the germination of Bambara groundnut seeds by 22%. LPP increases the wettability and roughness of the seed hilum while oxidizing the surface with carboxyl and amine groups. In this H2O-based treatment of Bambara groundnut seeds, combinatory etching and chemical modification facilitated the imbibition process and increased the germination percentage. The success of this method has the potential to be scaled up to solve food security with seeds otherwise facing germination-related issues.

scholarly journals Asymbiotic germination of Vanilla planifolia in relation to the timing of seed collection and seed pretreatments

2021 ◽  
Vol 62 (1) ◽  
Author(s):  
Chih-Hsin Yeh ◽  
Kai-Yi Chen ◽  
Yung-I. Lee
Keyword(s):  
Seed Germination ◽  
Seed Development ◽  
Seed Coat ◽  
Germination Percentage ◽  
Vanilla Planifolia ◽  
Outermost Layer ◽  
Seed Collection ◽  
Seed Pretreatment ◽  
Immature Seeds ◽  
Mature Seeds

Abstract Background Vanilla planifolia is an important tropical orchid for production of natural vanilla flavor. Traditionally, V. planifolia is propagated by stem cuttings, which produces identical genotype that are sensitive to virulent pathogens. However, propagation with seed germination of V. planifolia is intricate and unstable because the seed coat is extremely hard with strong hydrophobic nature. A better understanding of seed development, especially the formation of impermeable seed coat would provide insights into seed propagation and conservation of genetic resources of Vanilla. Results We found that soaking mature seeds in 4% sodium hypochlorite solution from 75 to 90 min significantly increased germination. For the culture of immature seeds, the seed collection at 45 days after pollination (DAP) had the highest germination percentage. We then investigated the anatomical features during seed development that associated with the effect of seed pretreatment on raising seed germination percentage. The 45-DAP immature seeds have developed globular embryos and the thickened non-lignified cell wall at the outermost layer of the outer seed coat. Seeds at 60 DAP and subsequent stages germinated poorly. As the seed approached maturity, the cell wall of the outermost layer of the outer seed coat became lignified and finally compressed into a thick envelope at maturity. On toluidine blue O staining, the wall of outer seed coat stained greenish blue, indicating the presence of phenolic compounds. As well, on Nile red staining, a cuticular substance was detected in the surface wall of the embryo proper and the innermost wall of the inner seed coat. Conclusion We report a reliable protocol for seed pretreatment of mature seeds and for immature seeds culture based on a defined time schedule of V. plantifolia seed development. The window for successful germination of culturing immature seed was short. The quick accumulation of lignin, phenolics and/or phytomelanins in the seed coat may seriously inhibit seed germination after 45 DAP. As seeds matured, the thickened and lignified seed coat formed an impermeable envelope surrounding the embryo, which may play an important role in inducing dormancy. Further studies covering different maturity of green capsules are required to understand the optimal seed maturity and germination of seeds.

scholarly journals Effect of Environmental Factors on the Germination and Emergence of Drunken Horse Grass (Achnatherum inebrians)

Weed Science ◽  
2020 ◽  
pp. 1-29
Author(s):  
Yonghuan Yue ◽  
Guili Jin ◽  
Weihua Lu ◽  
Ke Gong ◽  
Wanqiang Han ◽  
...  
Keyword(s):  
Seed Germination ◽  
Environmental Factors ◽  
Abiotic Factors ◽  
Seedling Emergence ◽  
Management Strategies ◽  
Germination Percentage ◽  
Western China ◽  
Burial Depth ◽  
Achnatherum Inebrians ◽  
Water Ph

Abstract Drunken horse grass [Achnatherum inebrians (Hance) Keng] is a perennial poisonous weed in western China. A comprehensive understanding of the ecological response of A. inebrians germination to environmental factors would facilitate the formulation of better management strategies for this weed. Experiments were conducted under laboratory conditions to assess the effects of various abiotic factors, including temperature, light, water, pH and burial depth, on the seed germination and seedling emergence of A. inebrians. The seeds germinated at constant temperatures of 15, 20, 25, 30, 35°C and in alternating-temperature regimes of 15/5, 20/10, 25/15, 30/20, 35/25, 40/30°C, and the seed germination percentages under constant and alternating temperatures ranged from 51% to 94% and 15% to 93%, respectively. Maximum germination occurred at a constant temperature of 25°C, and germination was prevented at 45/35°C. Light did not appear to affect seed germination. The germination percentage of seeds was more than 75% in the pH range of 5 to 10, with the highest germination percentage at pH 6. The seeds germinated at osmotic potentials of 0 MPa to -1.0 MPa, but decreasing osmotic potential inhibited germination, with no germination at -1.2MPa. After 21 d of low osmotic stress, the seeds that did not germinate after rehydration had not lost their vitality. The seedling emergence percentage was highest (90%) when seeds were buried at 1 cm but declined with increasing burial depth and no emergence at 9 cm. Deep tillage may be effective in limiting the seed germination and emergence of this species. The results of this study provide useful information on the conditions necessary for A. inebrians germination and provide a theoretical basis for science-based prediction, prevention and control of this species.

The Effect of High Concentrations of Ethylene on Seed Germination of Douglas Fir (Pseudotsugamenziesii (Mirb.) Franco)

1975 ◽  
Vol 5 (3) ◽  
pp. 419-423 ◽  
Author(s):  
Carey Borno ◽  
Iain E. P. Taylor
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Inhibitory Effect ◽  
Germination Percentage ◽  
Douglas Fir ◽  
Control Treatment ◽  
Continuous Exposure ◽  
Short Term ◽  
Short Term Exposure ◽  
High Concentrations

Stratified, imbibed Douglas fir (Pseudotsugamenziesii (Mirb.) Franco) seeds were exposed to 100% ethylene for times between 0 and 366 h. Germination rate and germination percentage were increased by treatments up to 48 h. The 12-h treatment gave largest stimulation; 30% enhancement of final germination percentage over control. Treatment for 96 h caused increased germination rate for the first 5 days but reduced the germination percentage. Germinants were subject to continuous exposure to atmospheres containing 0.1 – 200 000 ppm ethylene in air, but it did not stimulate growth, and the gas was inhibitory above 100 ppm. Although some effects of high concentrations of ethylene may have been due to the lowering of oxygen supplies, this alone was insufficient to account for the full inhibitory effect. The mechanism of stimulation by short-term exposure to ethylene is discussed.

Factors affecting red-osier dogwood seed germination

1992 ◽  
Vol 70 (5) ◽  
pp. 1012-1016 ◽  
Author(s):  
S. N. Acharya ◽  
C. B. Chu ◽  
R. Hermesh ◽  
G. B. Schaalje
Keyword(s):  
Seed Germination ◽  
Germination Percentage ◽  
Collection Site ◽  
Moisture Condition ◽  
Seed Formation ◽  
Factors Affecting ◽  
Year Effect ◽  
Wide Range ◽  
Cornus Stolonifera ◽  
Moisture Conditions

Seeds from 55 populations of native Alberta red-osier dogwood (Cornus stolonifera Michx.) were collected over 2 years (1984 and 1985) to determine the range of variability for seed germination and the effect of ecoregion and moisture conditions in the collection sites on this trait. Each year the unscarified seeds were stored at 3 °C for 30 days, stratified at 3 °C for an additional 30 days and then incubated on an 8 h light: 16 h dark cycle at a temperature of 25:10 °C (light:dark) for germination. A wide range of germination percentages was observed among populations collected in any 1 year. However, the variability was not due to the ecoregion or moisture condition of the collection site. Germination percentage of populations collected in 1984 and 1985 formed five and seven groups, respectively. A study involving eight sites that were common to 1984, 1985, and 1986 revealed a significant effect of population on seed germination. This population effect was not obscured by the combined effects of year, precipitation, temperature, and seed size. The year effect was significant only when population was used as a covariate. Precipitation during seed formation significantly influenced germination performance even after adjustments were made for population and year. Seed germination in red-osier dogwood appears to be influenced by the population from which the seeds are collected and by yearly environmental fluctuations. Therefore, germination studies in this species must include seeds from diverse populations collected over several years. Key words: red-osier dogwood, Cornus stolonifera Michx., germination, population, environment.

Chitosan coating on bean and maize seeds: release of agrochemical fungicide and post-storage condition

2021 ◽  
Vol 43 ◽  
Author(s):  
Nancy Araceli Godínez-Garrido ◽  
Juan Gabriel Ramírez-Pimentel ◽  
Jorge Covarrubias-Prieto ◽  
Francisco Cervantes-Ortiz ◽  
Artemio Pérez-López ◽  
...  
Keyword(s):  
Seed Germination ◽  
Defense Mechanisms ◽  
Germination Rate ◽  
Storage Condition ◽  
Germination Percentage ◽  
Negative Effects ◽  
Retention Capacity ◽  
Chitosan Coating ◽  
Maize Seeds ◽  
Germination Speed

Abstract: Chitosan is a biopolymer obtained from deacetylation of chitin; it has multiple applications in agriculture as an antifungal, soil conditioner, inducer of defense mechanisms, fruits postharvest coating, leaves and seeds, among others. The objective in this research was to evaluate the effect of chitosan coatings mixed with fungicide (dithiocarbamate) on the germination and germination speed of bean and maize seeds in storage and to determine the retention capacity of the fungicide in the coated seeds under different times of imbibition. Two coating treatments at concentrations of 0.1 and 0.5% chitosan in water, two coatings treatments at 0.1 and 0.5% chitosan supplemented with 0.5% fungicide and a coating without chitosan using only 0.5% fungicide in water were used in bean and maize seed; and as control seeds imbibed in distilled water were used; after treatments, germination percentage and germination speed were determined, also fungicide release were determined at 0, 1, 2 and 6 h of imbibition, and the effect of storage time on germination and germination speed was determined at 30, 60, 90, 120, 150 and 180 days of storage at 4 °C and 45% relative humidity. The fungicide release effect was determined by inhibiting Fusarium oxysporum conidia germination. There were no negative effects of coatings on seed germination after storage. The treatment that provided both greater retention of the fungicidal agent and released it gradually, was 0.5% chitosan mixed with fungicide concentration. Chitosan coating seeds mixed with fungicide do not cause negative changes in seed germination or germination rate.

Temperature Effect on Seed Germination Performance of a Local Variety of Onion in Bangladesh

2021 ◽  
Vol 1 (01) ◽  
pp. 27-30
Author(s):  
IRANI KHATUN ◽  
RIYAD HOSSEN
Keyword(s):  
Seed Germination ◽  
Temperature Effect ◽  
Performance Test ◽  
Germination Rate ◽  
Germination Percentage ◽  
Optimum Range ◽  
Local Variety ◽  
Different Temperatures ◽  
Local Farmers

Seed germination performance test of Taherpuri onion (a local variety of onion) under six different temperatures (15, 20, 25, 30, 35 and 40°C) was the main goal of this experiment. Germination percentage (GP) was calculated at highest 60.25% at 25°C, and the highest germination rate 20.08 was observed in the same temperature condition. The lowest germination performance (13.25 % germi-nation and 3.32 seeds per day as germination rate) was found at 40°C temperature. Finally, the authors mentioned the temperature 20 to 30°C as optimum range, and suggested the temperature 25°C as best suited for obtaining highest results in case of both germination percentage and germination rate of these seeds. To produce maximum seedlings of the local variety of onion, the mentioned temperature should be followed by the local farmers.

scholarly journals Acid and Temperature Treatments Result in Increased Germination of Seeds of Three Fescue Species

2012 ◽  
Vol 40 (2) ◽  
pp. 220 ◽  
Author(s):  
Rade S. STANISAVLJEVIC ◽  
Savo M. VUCKOVIC ◽  
Aleksandar S. SIMIC ◽  
Jordan P. MARKOVIC ◽  
Zelijco P. LAKIC ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Seed Germination ◽  
Acid Solution ◽  
Sulfuric Acid Solution ◽  
Germination Rate ◽  
Festuca Rubra ◽  
Time Intervals ◽  
Different Temperatures ◽  
Successful Establishment ◽  
Seed Collecting

Efficient germination of fescue seeds is essential for successful establishment of meadows and pastures. This research was conducted to ascertain the effects of various acid and temperature treatments on seed germination in three fescue species: Festuca rubra, F. ovina, and F. pratensis. Seeds from different cultivars, populations, or lots were exposed either to four concentrations of sulfuric acid at three different time intervals (12 treatments) or six different temperatures at three different time intervals (18 treatments). Despite all belonging to the genus Festuca, the seed from different species responded differently to the treatments. The three optimum treatments for F. rubra seed involved soaking in a 75% solution of sulfuric acid for 20 minutes (improved the germination rate by 19%), soaking in a 50% solution of sulfuric acid for 30 minutes (improved the germination rate by 18%) and exposure to either 60°C or 70°C for 90 minutes (improved the germination rate by 17%). For F. ovina seed, optimal treatments included soaking seeds for either 10 or 20 minutes in a 50% sulfuric acid solution (both treatments improved germination rates by 13%) or exposing seeds for 30 minutes in a 25% sulfuric acid solution and 80°C for 60 minutes (improved germination rate by 12%). Two optimal treatments were identified for F. pratensis seed. Whereas the first involved soaking the seeds in a 75% sulfuric acid solution for 30 minutes (improved germination rates by 22%), the second involved either exposing the seeds to 90°C for 90 or 60 minutes, or exposing the seeds to 80°C for 90 minutes (improved germination rate by 21%). Our findings indicate that if fescue seed is to be sown during the autumn (two to three months after seed collecting), treating it with acid and temperature can significantly enhance its germination.

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