scholarly journals Flutolanil for Control of Necrotic Fairy Rings on Bermudagrass Putting Greens

2002 ◽  
Vol 12 (4) ◽  
pp. 656-659 ◽  
Author(s):  
Monica L. Elliott ◽  
Robert B. Hickman ◽  
Mark Hopkins

Type 1 (necrotic) fairy rings in turfgrass result in dead or badly damaged grass. This type of fairy ring is a severe problem on golf course greens as they interfere with the aesthetics and playability of the putting surface. In Florida, Lycoperdon spp., basidiomycetes that produce puffball mushrooms, have been implicated as a common cause of Type 1 fairy rings on hybrid bermudagrass (Cynodon dactylon × C. transvaalensis) putting greens. The fungicide flutolanil has basidiomycetes as the sole fungal target. It is also the only carboxin-related fungicide registered for use on turfgrass. Two experiments were conducted to examine the effect of flutolanil as a curative and preventive treatment for fairy ring caused by Lycoperdon. One experiment, established after the rings were present, determined that flutolanil significantly reduced mushroom production. The second experiment was conducted on a golf course that had experienced Type 1 fairy rings previously. One-half of each of nine putting greens was treated with flutolanil on a preventive basis. The other half of each green served as an untreated control. Type 1 fairy rings, due to Lycoperdon, developed only on the untreated control half of each green. These experiments confirm that flutolanil does have curative and preventive activity against Lycoperdon spp. that cause Type 1 fairy rings.

HortScience ◽  
2008 ◽  
Vol 43 (2) ◽  
pp. 514-518 ◽  
Author(s):  
Monica L. Elliott ◽  
J.A. McInroy ◽  
K. Xiong ◽  
J.H. Kim ◽  
H.D. Skipper ◽  
...  

Taxonomic diversity of bacteria associated with golf course putting greens is a topic that has not been widely explored. The purpose of this project was to isolate and identify culturable bacteria from the rhizosphere of creeping bentgrass (Agrostris palustris Huds.) at two sites (Alabama and North Carolina) and hybrid bermudagrass [Cynodon dactylon (L.) Pers. × C. transvaalensis Burtt-Davy] at two sites (Florida and South Carolina) for a minimum of 3 years with sampling initiated after the construction process. Randomly selected colonies were identified using gas chromatography for analysis of fatty acid methyl ester profiles. Over 9000 isolates were successfully analyzed. When a similarity index of 0.300 or higher was used, the average number of unidentifiable isolates was 38.6%. The two dominant genera in both bentgrass and bermudagrass rhizospheres were Bacillus and Pseudomonas with Bacillus dominant in bermudagrass and Pseudomonas dominant or equal to Bacillus in bentgrass. Other genera that comprised at least 1% of the isolates at all four sites were Clavibacter, Flavobacterium, and Microbacterium. Arthrobacter also comprised a significant portion of the bacterial isolates in the bentgrass rhizosphere, but not the bermudagrass rhizosphere. Overall, there were 40 genera common to all four sites. At the species level, there were five that comprised at least 1% of the isolates at each location: B. cereus, B. megaterium, C. michiganensis, F. johnsoniae, and P. putida. As has been reported for many grasses, we found considerable taxonomic diversity among the culturable bacterial populations from the rhizospheres of bentgrass and bermudagrass grown in sand-based putting greens.


Plant Disease ◽  
2013 ◽  
Vol 97 (4) ◽  
pp. 561-561 ◽  
Author(s):  
W. Zhang ◽  
Z. B. Nan ◽  
G. D. Liu

Hybrid bermudagrass (Cynodon dactylon (L.) Pers. × C. transvaalensis Burtt-Davy) is widely used on golf course putting greens in southern China. In September 2011, circular pink patches ranging from 10 to 20 cm in diameter were observed on putting greens established with cv. ‘Tifgreen’ on a golf course in Haikou, Hainan Province. There were approximately 50 pink patches on a putting green. Infected leaves were covered with pink, gelatinous fungal mycelium, which resulted in the production of chlorotic lesions. Lesions expanded, became water-soaked, and leaves died basipetally. A pink fungus, characterized by the presence of clamp connections, was consistently isolated from leaves of infected plants on a potato dextrose agar amended with 0.01% gentamicin sulfate. Based on morphological characteristics, the fungus was preliminary identified as Limonomyces roseipellis Stalpers & Loerakker, the causal agent of pink patch of turfgrass (2,3). To verify the identity, the internal transcribed spacer (ITS) of rDNA was amplified and sequenced using primers ITS1 and ITS4. Comparison with sequences in the GenBank database revealed that the ITS sequence (Accession No. KC193592) showed 98% homology with the sequence of L. roseipellis (EU622846). For pathogenicity tests, inoculum was prepared by culturing the fungus on an autoclaved mixture of 100 g of rye grain and 20 ml water for 3 weeks at 25°C. Six-week-old C. dactylon plants in 10-cm pots were inoculated by placing 2 g of infested grain in the center of the turf canopy, or 2 g sterilized, uninfested grain as a control, with four replications of each treatment. After inoculation, pots were covered with translucent plastic bags and placed in a greenhouse at 24 ± 2°C with a 12-h photoperiod (1). After 3 weeks, more than 70% of leaves in the infested pots showed symptoms identical to those observed under natural conditions while control plants remained asymptomatic. The fungus was reisolated from symptomatic plants. To our knowledge, this is the first report of L. roseipellis causing pink patch on hybrid bermudagrass in China. References: (1) L. L. Burpee and L. G. Goulty. Phytopathology 74:692, 1986. (2) J. D. Kaplan and N. Jackson. Plant Dis. 67:159, 1983. (3) J. A. Stalpers and W. M. Loerakker. Can. J. Bot. 60:529, 1982.


Planta ◽  
2016 ◽  
Vol 244 (4) ◽  
pp. 761-773 ◽  
Author(s):  
Eric H. Reasor ◽  
James T. Brosnan ◽  
Robert N. Trigiano ◽  
J. Earl Elsner ◽  
Gerald M. Henry ◽  
...  

Plant Disease ◽  
2006 ◽  
Vol 90 (8) ◽  
pp. 1114-1114 ◽  
Author(s):  
J. E. Kaminski ◽  
T. Hsiang

Dead spot, also known as bentgrass dead spot or bermudagrass dead spot, is a relatively new disease of golf course putting greens and is caused by the pathogen Ophiosphaerella agrostis (1). The disease first was reported on a creeping bentgrass (Agrostis stolonifera) putting green in Maryland (2) and since has been identified on putting greens of creeping bentgrass and hybrid bermudagrass (Cynodon dactylon × C. transvaalensis) in the eastern and southern United States (3,4). In June 2004, disease symptoms resembling dead spot were observed on a golf course in southern Ontario. Small (≤3 cm) spots first appeared approximately 14 months after establishment of the sand-based, ‘L-93’ creeping bentgrass putting greens. The disease became more severe during the summer months and patches increased in size to as much as 5 to 8 cm in diameter. Dead spot infection centers remained visible throughout the winter months and the disease again became active during the spring of 2005. Bentgrass tissues growing adjacent to the periphery of active infection centers were orange-red to reddish-brown. Although dark brown ectotrophic hyphae were observed on bentgrass stolons, none were found on the roots. Few new infection centers occurred in 2005 and pseudothecia embedded within necrotic tissue only were observed in small numbers. No mature ascospores were observed when samples were collected during September 2005. A single fungal morphotype consistently was isolated from leaves and stolons with a rose-quartz color when grown for several days on potato dextrose agar. To demonstrate pathogenicity, ‘L-93’ creeping bentgrass seedlings were grown for 28 days in 10-cm-diameter pots containing an autoclaved greens-mix with a mechanical analysis of 94% sand, 5% silt, and 1% clay. Inoculum was prepared by placing mycelia from a hyphal-tipped isolate on an autoclaved mix of seed of tall fescue (Festuca arundinacea) and wheat (Triticum aestivum) bran (50% [vol/vol]), and grown at 24°C for 14 days. The inoculum (5 g) was embedded a few milliliters into the sand in the center of each pot (n = 5), and uninfested inoculum served as the untreated control. Pots were placed in enclosed plastic containers and incubated at room temperature (13 to 26°C) under natural light (replication 1) or under 14 h of light per day from fluorescent lights (replication 2). After 7 days, tissue along the periphery of each inoculation point became covered in a pink mycelium, and newly infected leaves appeared tan or brownish-red. Most plants were dead after 22 to 28 days of incubation. Reisolation of the pathogen from necrotic leaves produced fungal colonies similar in color, morphology, and growth rate to the original isolates. Few pseudothecia developed on infected tissue but were present in large numbers on infested tall fescue seed. Bitunicate asci containing spirally twisted filiform ascospores were observed. Light brown ascospores (n = 50) were 7 to 15 septate and measured 1.9 to 3.6 μm × 60.7 to 147.9 μm. On the basis of field symptoms, morphological characteristics, and pathogenicity tests, the pathogen was identified as O. agrostis. To our knowledge, this is the first report of dead spot on creeping bentgrass in Canada and of O. agrostis outside the United States. References: (1) M. P. S. Câmara et al. Mycologia 92:317, 2000. (2) P. H. Dernoeden et al. Plant Dis. 83:397, 1999. (3) J. E. Kaminski and P. H. Dernoeden. Plant Dis. 86:1253, 2002. (4) J. P. Krausz et al. Plant Dis. 85:1286, 2001.


2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Yan Wang ◽  
Lin Xing ◽  
Hui Yu ◽  
LiJuan Zhao

Abstract Background Dental caries and type 1 diabetes are responsible for a large burden of global disease; however, the exact prevalence of dental caries among children and adolescents with type 1 diabetes remains controversial, and no quantitative meta-analysis exists. Thus, we performed a meta-analysis to evaluate the prevalence of dental caries among children and adolescents with type 1 diabetes. Methods We performed a systematic search strategy using PubMed, EMBASE and China National Knowledge Infrastructure for relevant studies investigating the prevalence of dental caries in children and adolescents with type 1 diabetes from July 1971 until December 2018. The pooled prevalence with 95% confidence intervals (95%CIs) and subgroup analyses were calculated using a random effects model. Results After screening 358 non-duplicated articles, a total of 10 articles involving 538 individuals were included. The overall prevalence of dental caries among children and adolescents with type 1 diabetes was 67% (95% CI: 0.56–0.77%; I2 = 83%). The prevalence was highest in South America (84%) and lowest in diabetic patients with good metabolic control (47%). Conclusions The prevalence of dental caries was high among children and adolescents with type 1 diabetes. Screening and preventive treatment should be included in dental clinical routines for diabetic children and adolescents, especially in those with poor metabolic control.


2005 ◽  
Vol 2 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Brian P. Boesch ◽  
Nathaniel A. Mitkowski

Plant Disease ◽  
2007 ◽  
Vol 91 (7) ◽  
pp. 905-905 ◽  
Author(s):  
N. A. Mitkowski

In the fall of 2006, a golf course in Snoqualmie, WA renovated five putting greens with commercially produced Poa annua L. sod from British Columbia, Canada. Prior to the renovation, the greens had been planted with Agrostis stolonifera L. cv. Providence, which was removed during the renovation. In February of 2007, chlorotic patches were observed on the newly established P. annua greens. When the roots were examined, extensive galling was observed throughout plant roots. Galls were slender and twisted in appearance and less than one millimeter long. Upon dissection of washed galls, hundreds of eggs were exuded into the surrounding water droplet and both mature male and female nematodes were observed. Further morphometric examination of males, females, and juvenile nematodes demonstrated that they were Subanguina radicicola (Greef 1872) Paramanov 1967 (1). Amplification of nematode 18S, ITS1, and 5.8S regions, using previously published primers (2), resulted in a 100% sequence match with the publicly available sequence for S. radicicola, GenBank Accession No. AF396366. Each P. annua plant had an average of six galls (with a range of 1 to 8), primarily located within the top 2 cm of the soil. All five new P. annua putting greens at the golf course were infested with the nematode. Additionally, P. annua from two A. stolonifera cv. Providence greens that had not been renovated was infected, suggesting that the population occurred onsite and was not imported from the Canadian sod. S. radicicola has been identified as causing severe damage in New Brunswick, Canada on P. annua putting greens and in wild P. annua in the northwestern United States, but to our knowledge, this is the first report of the nematode affecting P. annua on a golf course in the United States. References: (1) E. L. Krall. Wheat and grass nematodes: Anguina, Subanguina, and related genera. Pages 721–760 in: Manual of Agricultural Nematology. Marcel Dekker, New York, 1991. (2) N. A. Mitkowski et al. Plant Dis. 86:840, 2002.


2020 ◽  
Vol 26 (3) ◽  
pp. 422-431 ◽  
Author(s):  
Caroline de Moura D’Andréa Mateus ◽  
Regina Maria Monteiro de Castilho ◽  
Patrick Luan Ferreira dos Santos ◽  
Flávia Diniz Mota ◽  
Leandro José Grava de Godoy ◽  
...  

Abstract One of the management techniques used in greens (final areas of the holes) of golf course is the fertilizer. To correct fertilization program of these areas should know the consumption of nutrients throughout the plant cycle. The objective was to determine the export of nutrients by clipping of Tifdwarf bermudagrass (Cynodon dactylon (L.) Pers. x Cynodon transvaalensis Burtt-Davey) used in golf courses greens in the four weather seasons, as information to improve nutritional management. The research project was conducted in two golf courses in Sao Paulo State. Four greens were studied in each field, for a period of one year, using a 4x2 factorial (weather seasons x golf courses) in a completely randomized design. The production of dry matter and nutrient concentration were evaluated, and the absorption and exportation of nutrients by leaf clippings was subsequently calculated. The amount of nutrients exported by turfgrass clippings was different between the two golf courses. The summer was the season of greatest accumulation and export of nutrients in FG, except for Fe. Autumn was the season of greatest accumulation and export of nutrients in FCA, except for Mn and Zn. Considering the amount of nutrients exported by the greens clippings of the Tifdwarf bermudagrass from the two golf courses studied, the macronutrients were exported in the following order: N>K>Ca>P>S>Mg (21.8, 4.3, 2.0, 1.9, 1.8, 0.7 g m-2) and the micronutrients were exported in the following order: Fe>Zn>Mn>Cu>B (257.8, 27.4, 23.6, 7.8, 6.0 mg m-2).


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