scholarly journals 575 PB 050 EFFICIENT PLANT REGENERATION FROM LEAF AND PETIOLE EXPLANTS IN RED RASPBERRY

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 514b-514 ◽  
Author(s):  
Cynthia Cohen ◽  
H. Mathews ◽  
V. Dewey ◽  
R. Bestwick
Keyword(s):  
Plant Regeneration ◽  
Average Rate ◽  
Leaf Explants ◽  
Rubus Idaeus ◽  
Regeneration System ◽  
Red Raspberry ◽  
Petiole Explants ◽  
Leaf Petiole ◽  
Rooting Medium ◽  
Modified Ms Medium

Raspberry has very cultivar specific requirements for proliferation. Plant regeneration rates from isolated explants are inconsistent and vary widely among cultivars. As a step towards developing a viable transgenic system in red raspberry (Rubus idaeus L.) we first developed an efficient and consistent protocol for plant regeneration from isolated explants. A modified MS medium with cytokinin BA gave vigorous shoots with an average proliferation rate of 3-5 depending on the cultivar. These vigorous shoot proliferants served as an ideal explant source for plant regeneration experiments. The average rate of shoot regeneration from leaf explants was 72, 32, 68. and 72% for cvs. Canby, Chilliwack, Meeker and Heritage respectively. In addition to leaf, petiole explants were equally good sources for inducing shoot organogenesis. In all the above-mentioned cultivars, 44-57% of the petiole explants gave rise lo healthy and vigorous shoot regenerants in culture. The regenerated shoots were induced lo root on a rooting medium and were successfully transplanted to the greenhouse. This regeneration system was successfully applied in our laboratory for developing gene transfer system in red raspberry (see abstract by Mathews, et al).

scholarly journals Establishment of plant regeneration system for young leaf explants of Cassia mimosoides

2021 ◽  
Vol 245 ◽  
pp. 03067
Author(s):  
Yali Yang ◽  
Peiting He ◽  
Shengyuan Zhang ◽  
Dadu Lin ◽  
Zhentao Liu ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Leaf Explants ◽  
Young Leaf ◽  
High Rate ◽  
Adventitious Bud ◽  
Good Growth ◽  
Regeneration System ◽  
Root Regeneration ◽  
Rooting Medium ◽  
Adventitious Bud Regeneration

In the present study, a plant regeneration system was established by using young leaf explants from aseptic seedlings of Cassia mimosoides. The results showed that when the explants were inoculated on the 1/2MS medium contained 2.0 mg/L 6-BA and 0.5 mg/L NAA, high rate of callus induction and good growth status of adventitious bud, and the highest number of buds were obtained. Further studies conclusively suggested that IBA could not promote root formation effectively, but the root regeneration could be induced by NAA. However, although the rooting of regeneration buds could be induced when adventitious buds were placed into 1/2MS medium supplemented with 0.5 mg/L NAA, the rate of rooting was reduced, and the roots became shorter. Therefore, the best medium for adventitious bud regeneration using young leaf as explants in C. mimosoides was 1/2MS medium contained 2.0 mg/L 6-BA and 0.5 mg/L NAA, and the best rooting medium was 1/2MS.

Characterization and plant regeneration of cell suspension cultures of Lycopersicon chilense

1991 ◽  
Vol 69 (10) ◽  
pp. 2257-2260 ◽  
Author(s):  
Ann Francine Greer ◽  
Zohreh Tabaeizadeh
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Suspensions ◽  
Lag Phase ◽  
Petiole Explants ◽  
Lycopersicon Chilense

To produce calli for the establishment of a cell suspension, leaf, stem, and petiole explants of Lycopersicon chilense Dun., grown in vitro and in the soil, were cultured on media containing 15 different combinations of benzylaminopurine, kinetin, and indole acetic acid. Among the three types of tissues, leaf explants showed the best response. Cell suspension cultures of L. chilense were established from leaf callus derived from soil grown plants using Murashige and Skoog's medium supplemented with casein hydrolysate (250 mg/L), coconut water (5%), and 2,4-dichlorophenoxyacetic acid (2 mg/L). Once established, cell suspensions showed a rapid growth rate with no marked lag phase. Shooting via organogenesis occurred from callus derived from cell suspensions on medium containing 2 mg/L benzylaminopurine. Regenerated plants had the same morphology as the original plants. Key words: Lycopersicon chilense, tomato, tissue culture, cell suspensions, organogenesis, plant regeneration.

scholarly journals Development of an Efficient Plant Regeneration System for the Selenium-hyperaccumulator Astragalus racemosus and the Nonaccumulator Astragalus canadensis

HortScience ◽  
2008 ◽  
Vol 43 (7) ◽  
pp. 2138-2142 ◽  
Author(s):  
Chiu-Yueh Hung ◽  
Jiahua Xie
Keyword(s):  
Plant Regeneration ◽  
Basal Medium ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Naphthaleneacetic Acid ◽  
Shoot Induction ◽  
Regeneration System ◽  
Rooting Medium ◽  
Significant Difference

A method of in vitro plant regeneration for both the selenium-hyperaccumulator Astragalus racemosus ‘Cream Milkvetch’ and the nonaccumulator Astragalus canadensis ‘Canadian Milkvetch’ was developed with two induction media, M1 and M2. The M1 and M2 contain Murashige and Skoog basal medium plus vitamins, 8.07 μm N-(2-chloro-4-pyridyl)-N′-phenylurea, 2.5% (w·v−1) sucrose, 0.7% (w·v−1) agar (pH 5.7), and 0.89 μm or 3.12 μm a-naphthaleneacetic acid, respectively. In vitro cultures were initiated on these two types of media with three types of explants: cotyledons, hypocotyls, and roots. More than 93% of cultured explants from both species could form calli or calli with shoots. With regard to shoot formation, A. canadensis could produce multiple shoots from all types of explants more efficiently than A. racemosus. The highest shoot induction was approximately three shoots per explant in A. racemosus, whereas A. canadensis could reach ≈10 shoots per explant. M1 could induce more shoots than M2 no matter what type of explant was used, but the overall induction rates were no significant difference. Among the three types of explants used, the cotyledons were the best explants for shoot induction in A. canadensis, whereas hypocotyls were the best in A. racemosus. In A. racemosus, shoots could also be obtained from calli on the rooting medium containing Murashige and Skoog basal plus vitamins, 2.84 μm indole-3 acetic acid, 2.5% (w·v−1) sucrose, and 0.7% (w·v−1) agar (pH 5.7). Approximately 43% of A. canadensis shoots and 19% of A. racemosus shoots could be rooted on the rooting medium.

scholarly journals Plant regeneration via somatic embryogenesis from immature leaves in Tetrapleura tetraptera (Schum. & Thonn.) Taub.

2011 ◽  
Vol 63 (4) ◽  
pp. 1135-1145 ◽  
Author(s):  
J.T. Opabode ◽  
O.A. Akinyemiju ◽  
O.O. Ayeni
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Leaf Explants ◽  
Basal Medium ◽  
Shoot Elongation ◽  
Leaf Petiole ◽  
Immature Leaf ◽  
Embryogenic Calluses ◽  
Tetrapleura Tetraptera ◽  
Secondary Embryos

Plant regeneration via somatic embryogenesis was assessed using immature leaf, petiole and apical meristem explants in Tetrapleura tetraptera. Somatic embryos were induced in the immature leaf using MS basal medium supplemented with 2,4-D and matured on MS basal medium containing BAP. Medium supplemented with 12 mg/l 2,4-D had the highest (43.1%) percentage of embryogenic calluses from immature leaf explants. Conversion of embryogenic callus to mature primary somatic embryo occurred in the medium that contained 1.2 mg/l BAP. Development of secondary embryogenic calluses to matured secondary embryos was highest (98.0%) in the medium with 0.4 mg/l BAP, while the highest average number of mature secondary embryos (6.0) was obtained in the same medium. Medium supplemented with 1.0 mg/l BAP and 0.5 mg/l IBA had the highest (38.7%) percentage of explants with shootbuds. The highest (18.1%) percentage of shoot elongation was obtained in medium with 1.0 mg/l BAP and 20 mg/l IBA. Shootbuds survived and produced roots on medium free of plant growth regulators. Shoots obtained on medium supplemented with 1.0 mg/l BAP and 20 mg/g IBA recorded the highest number of roots per plantlet (7.5) with no apparent morphological abnormality.

scholarly journals Efficient plant regeneration of bittersweet (Solanum dulcamara L.), a medicinal plant

2011 ◽  
Vol 77 (4) ◽  
pp. 275-280 ◽  
Author(s):  
Arzu Ucar Turker ◽  
Esra Canserver Multu
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Plant Growth ◽  
Medicinal Plant ◽  
Skin Diseases ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Solanum Dulcamara ◽  
Petiole Explants ◽  
Indole 3 Acetic Acid

<em>Solanum dulcamara</em> L. (bittersweet) is a medicinal plant that has been used to treat skin diseases, warts, tumors, felons, arthritis, rheumatism, bronchial congestion, heart ailments, ulcerative colitis, eye inflammations, jaundice and pneumonia. A reliable in vitro culture protocol for bittersweet was established. Explants (leaf and petiole segments) were cultured on Murashige and Skoog minimal organics (MSMO) medium with various plant growth regulator combinations. Leaf explants formed more shoots than petiole explants. Plant regeneration was observed through indirect organogenesis with both explants. Best shoot proliferation was obtained from leaf explants with 3 mg/l BA (benzyladenine) and 0.5 mg/l IAA (indole-3-acetic acid). Regenerated shoots were transferred to rooting media containing different levels of IAA (indole-3-acetic acid), IBA (indole-3-butyric acid), NAA (naphthalene acetic acid) or 2,4-D (2,4 dichlorophenoxyacetic acid). Most shoots developed roots on medium with 0.5 mg/l IBA. Rooted explants were transferred to vermiculate in Magenta containers for acclimatization and after 2 weeks, they were planted in plastic pots containing potting soil and maintained in the plant growth room.

scholarly journals 180 GENETIC TRANSFORMATION OF RED RASPBERRY (RUBUS IDAEUS L.) WITH A GENE TO CONTROL ETHYLENE BIOSYNTHESIS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 454f-454 ◽  
Author(s):  
Helena Mathews ◽  
C. Cohen ◽  
W. Wagoner ◽  
J. Kellogg ◽  
V. Dewey ◽  
...  
Keyword(s):  
Marker Gene ◽  
Ethylene Biosynthesis ◽  
Rubus Idaeus ◽  
Selection Marker ◽  
Red Raspberry ◽  
Leaf Petiole ◽  
Selection Medium ◽  
Recycling Pathway ◽  
Transformation Systems ◽  
First Time

We have developed efficient plant rageneration and transformation systems for red raspberry (Rubus idaeus L.). We have successfully introduced a gene for controlling biosynthesis of ethylene into raspberry for the first time. Leaf and petiole segments were co-cultivated with disarmed Agrobacterium strains EHA 101 or 105 containing plasmids pAG5420, pAG 1452 or pAG1552. The plasmids encoded gene sequences for S-adenosylmethionine hydrolase (SAM ase) driven by the fruit specific or wound and fruit specific tomato SE8 or E4 promoters. SAM ase catalyses the conversion of S-adenosylmethionine (SAM) to methylthioadenosine (MTA) and homoserine which can reenter the methionine recycling pathway. SAM is therefore not available for the synthesis of 1-am inocyclopropane carboxylic acid (ACC), the metabolic precursor for ethylene biosynthesis. Initial shoot regenerants were mostly chimeras containing transformed and non-transformed cells. Solid clones of pure transgenics were developed by repeated culture of leaf, petiole and nodal explants of primary regenerants on higher stringency selection medium. Transformants were screened on medium with kanamycin, geneticin or hygromycin depending on the selection marker gene NPTII or hpt. Genomic integration of transgenes were confirmed by Southern hybridization. Transgenic plants of cultivars Canby, Meeker and Chilliwack have been transplanted to the greenhouse for fruit set and further evaluation of transgenic traits.

scholarly journals Direct and Efficient Plant Regeneration from Different Explants Sources of Potato Cultivars as Influenced by Plant Growth Regulators

2012 ◽  
Vol 12 ◽  
pp. 1-6 ◽  
Author(s):  
Shambhu P. Dhital ◽  
Hak T. Lim ◽  
Hira K. Manandhar
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Solid Medium ◽  
Naphthaleneacetic Acid ◽  
Regeneration System ◽  
Petiole Explants ◽  
Number Of Shoots

Response of widely grown potato cv. Superior and newly developed cvs. Gui valley and Bora valley to plant growth regulators (PGRs) for direct plant regeneration from internode, leaf blade and petiole explants were investigated. The explants were cultured on a MS solid medium supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA), zeatin, indole-3-acetic acid (IAA) and gibberellic acid (GA3). Potato cv. Superior, regenerated direct shoot without callus and root formation on MS solid medium supplemented with BAP or zeatin, proliferous roots were produced on NAA or IAA supplemented medium and only some calli were produced on GA3 supplemented medium. The regeneration response varied with different concentrations of PGRs, singly and also in combinations. In the case of combined application of PGRs, the highest shoot regeneration (75.3%) and number of shoot per explant (11.5) and number of roots per explant (7.0) were obtained from the MS solid medium supplemented with zeatin (2 mg l-1), NAA (0.1 mg l-1) and GA3 0.1 mg l-1). Among the three types of explants evaluated, internodes produced the highest number of shoots and roots for both potato cvs. Gui valley and Bora valley, and petiole produced the least number of shoots and roots. The regenerated shoots were rooted in PGRs-free MS solid medium and successfully established under glasshouse condition. Leaf, flower, and tuber morphology were identical to in vitro control and mother plants in the same conditions. This optimized regeneration system can be used for rapid shoot proliferation and also for gene transformation.DOI: http://dx.doi.org/10.3126/njst.v12i0.6471 Nepal Journal of Science and Technology 12 (2011) 1-6 

scholarly journals Establishment of high frequency plant regeneration system from leaf explants of Pinellia koreana via bulblets formation

2009 ◽  
Vol 36 (2) ◽  
pp. 193-196 ◽  
Author(s):  
Myung-Jin Oh ◽  
Jong-Mi Park ◽  
Bu-Youn Lee ◽  
Pil-Son Choi ◽  
Kyoung-Hwan Tae ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Leaf Explants ◽  
Regeneration System

scholarly journals Plant Regeneration of Chokecherry (Prunus virginiana L.) from in vitro Leaf Tissues

2004 ◽  
Vol 22 (4) ◽  
pp. 225-228 ◽  
Author(s):  
Wenhao Dai ◽  
Victoria Jacques ◽  
James A. Walla ◽  
Zong-Ming Cheng
Keyword(s):  
Plant Regeneration ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Regeneration System ◽  
Prunus Virginiana ◽  
Leaf Tissues ◽  
Half Strength ◽  
Eventual Death ◽  
Roots In Vitro

Abstract An effective plant regeneration system was developed for chokecherry (Prunus virginiana L.) by using in vitro leaf tissues. Adventitious shoots regenerated from in vitro leaf tissues only when cultured on Woody Plant Medium (WPM), but not on Murashige and Skoog medium, supplemented with benzyladenine (BA) or thidiazuron (TDZ). Three chokecherry clones (NN, 10, and 17) responded differently to types and concentrations of cytokinins, ranging from 16.7 to 91.7% leaf explants regenerating shoots. A mean of four shoots was produced from each explant, with the most shoots (&gt; 10) from clone NN on media with 5–10 μM BA. Higher concentrations of TDZ (&gt; 8 μM) caused serious vitrification and eventual death of newly induced shoots. Regenerated shoots (&gt; 1.5 cm) produced roots in vitro in half strength MS medium or ex vitro in Cellular Rooting Sponge (CRS) rooting plugs with or without auxin (NAA or IBA) treatments. Rooting was affected by auxin, genotypes, and the rooting methods.

scholarly journals In Vitro Hardening of Red Raspberry by CO2 Enrichment and Reduced Medium Sucrose Concentration

HortScience ◽  
1993 ◽  
Vol 28 (10) ◽  
pp. 1048-1051 ◽  
Author(s):  
Ribo Deng ◽  
Danielle J. Donnelly
Keyword(s):  
Sucrose Concentration ◽  
Control Plant ◽  
Rubus Idaeus ◽  
Ex Vitro ◽  
Plant Leaves ◽  
Red Raspberry ◽  
Rooting Medium ◽  
Plantlet Growth ◽  
Greenhouse Control

Micropropagated `Festival' red raspberry (Rubus idaeus L.) shoots were rooted in specially constructed plexiglass chambers in ambient (340 ± 20 ppm) or enriched (1500 ±50 ppm) CO2 conditions on a medium containing 0, 10, 20, or 30 g sucrose/liter. Plantlet growth and leaf 14CO2 fixation rates were evaluated before and 4 weeks after ex vitro transplantation. In vitro CO2 enrichment promoted in vitro hardening; it increased root count and length, plantlet fresh weight, and photosynthetic capacity but did not affect other variables such as plantlet height, dry weight, or leaf count and area. No residual effects of in vitro CO2 enrichment were observed on 4-week-old transplants. Sucrose in the medium promoted plantlet growth but depressed photosynthesis and reduced in vitro hardening. Photoautotrophic plantlets were obtained on sucrose-free rooting medium under ambient and enriched CO2 conditions and they performed better ex vitro than mixotrophi plantlets grown with sucrose. Root hairs were more abundant and longer on root tips of photoautotrophic plantlets than on mixotrophic plantlets. The maximum CO2 uptake rate of plantlet leaves was 52% that of greenhouse control plant leaves. This did not change in the persistent leaves up to 4 weeks after ex vitro transplantation. The photosynthetic ability of persistent and new leaves of 4-week-old ex vitro transplants related neither to in vitro CO2 nor medium sucrose concentration. Consecutive new leaves of transplants took up more CO2 than persistent leaves. The third new leaf of transplants had photosynthetic rates up to 90% that of greenhouse control plant leaves. These results indicate that in vitro CO2 enrichment was beneficial to in vitro hardening and that sucrose may be reduced substantially or eliminated from red raspberry rooting medium when CO2 enrichment is used.

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