Human Neural Cell-Based Biosensor

2013 ◽  
Author(s):  
Steven L. Stice ◽  
Jamie Chilton
Keyword(s):  
Neural Cell ◽  
Human Neural Cell

Human Neural Cell-Based Biosensor

2012 ◽  
Author(s):  
Steven L. Stice ◽  
Jamie Chilton
Keyword(s):  
Neural Cell ◽  
Human Neural Cell

Acute and Persistent Infection of Human Neural Cell Lines by Human Coronavirus OC43

1999 ◽  
Vol 73 (4) ◽  
pp. 3338-3350 ◽  
Author(s):  
Nathalie Arbour ◽  
Geneviève Côté ◽  
Claude Lachance ◽  
Marc Tardieu ◽  
Neil R. Cashman ◽  
...  
Keyword(s):  
Nervous System ◽  
Cell Lines ◽  
Persistent Infection ◽  
Viral Antigen ◽  
Primary Cultures ◽  
Point Mutations ◽  
Neural Cell ◽  
Persistently Infected ◽  
Human Neural Cell ◽  
Neural Cell Lines

ABSTRACT Human coronaviruses (HuCV) are recognized respiratory pathogens. Data accumulated by different laboratories suggest their neurotropic potential. For example, primary cultures of human astrocytes and microglia were shown to be susceptible to an infection by the OC43 strain of HuCV (A. Bonavia, N. Arbour, V. W. Yong, and P. J. Talbot, J. Virol. 71:800–806, 1997). We speculate that the neurotropism of HuCV will lead to persistence within the central nervous system, as was observed for murine coronaviruses. As a first step in the verification of our hypothesis, we have characterized the susceptibility of various human neural cell lines to infection by HuCV-OC43. Viral antigen, infectious virus progeny, and viral RNA were monitored during both acute and persistent infections. The astrocytoma cell lines U-87 MG, U-373 MG, and GL-15, as well as neuroblastoma SK-N-SH, neuroglioma H4, oligodendrocytic MO3.13, and the CHME-5 immortalized fetal microglial cell lines, were all susceptible to an acute infection by HuCV-OC43. Viral antigen and RNA and release of infectious virions were observed during persistent HuCV-OC43 infections (∼130 days of culture) of U-87 MG, U-373 MG, MO3.13, and H4 cell lines. Nucleotide sequences of RNA encoding the putatively hypervariable viral S1 gene fragment obtained after 130 days of culture were compared to that of initial virus input. Point mutations leading to amino acid changes were observed in all persistently infected cell lines. Moreover, an in-frame deletion was also observed in persistently infected H4 cells. Some point mutations were observed in some molecular clones but not all, suggesting evolution of the viral population and the emergence of viral quasispecies during persistent infection of H4, U-87 MG, and MO3.13 cell lines. These results are consistent with the potential persistence of HuCV-OC43 in cells of the human nervous system, accompanied by the production of infectious virions and molecular variation of viral genomic RNA.

scholarly journals Amyloid-β42/40 ratio drives tau pathology in 3D human neural cell culture models of Alzheimer’s disease

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Sang Su Kwak ◽  
Kevin J. Washicosky ◽  
Emma Brand ◽  
Djuna von Maydell ◽  
Jenna Aronson ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Cell Culture ◽  
Neural Cell ◽  
Tau Pathology ◽  
Culture Models ◽  
Cell Culture Models ◽  
Human Neural Cell

Interleukin-15, a T-cell growth factor, is expressed in human neural cell lines and tissues

1998 ◽  
Vol 155 (2) ◽  
pp. 170-177 ◽  
Author(s):  
Jun-ichi Satoh ◽  
Kazuhiro Kurohara ◽  
Motohiro Yukitake ◽  
Yasuo Kuroda
Keyword(s):  
T Cell ◽  
Growth Factor ◽  
Cell Growth ◽  
Cell Lines ◽  
Neural Cell ◽  
Interleukin 15 ◽  
T Cell Growth ◽  
Human Neural Cell ◽  
Neural Cell Lines ◽  
T Cell Growth Factor

CD24, a glycosylphosphatidylinositol-anchored molecule, is transiently expressed during the development of human central nervous system and is a marker of human neural cell lineage tumors

1996 ◽  
Vol 91 (4) ◽  
pp. 400-408 ◽  
Author(s):  
Christian Poncet ◽  
Véronique Frances ◽  
Roberto Gristina ◽  
Claudie Scheiner ◽  
Jean-François Pellissier ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Cell Lineage ◽  
Neural Cell ◽  
Human Central Nervous System ◽  
Human Neural Cell
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