Characterization of Human Mammary Epithelial Stem Cells

2008 ◽  
Author(s):  
Peter D. Eirew
Keyword(s):  
Stem Cells ◽  
Mammary Epithelial ◽  
Epithelial Stem Cells ◽  
Human Mammary Epithelial

A method for quantifying normal human mammary epithelial stem cells with in vivo regenerative ability

2008 ◽  
Vol 14 (12) ◽  
pp. 1384-1389 ◽  
Author(s):  
Peter Eirew ◽  
John Stingl ◽  
Afshin Raouf ◽  
Gulisa Turashvili ◽  
Samuel Aparicio ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mammary Epithelial ◽  
Epithelial Stem Cells ◽  
Human Mammary Epithelial ◽  
Normal Human ◽  
Regenerative Ability

scholarly journals Derivation and Characterization of EGFP-Labeled Rabbit Limbal Mesenchymal Stem Cells and Their Potential for Research in Regenerative Ophthalmology

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1134
Author(s):  
Julia I. Khorolskaya ◽  
Daria A. Perepletchikova ◽  
Daniel V. Kachkin ◽  
Kirill E. Zhurenkov ◽  
Elga I. Alexander-Sinkler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Fluorescent Protein ◽  
Rabbit Model ◽  
Stem Cell Markers ◽  
Epithelial Stem Cells ◽  
Limbal Stem Cell ◽  
Green Fluorescent

The development of cell-based approaches to the treatment of various cornea pathologies, including limbal stem cell deficiency (LSCD), is an area of current interest in regenerative biomedicine. In this context, the shortage of donor material is urgent, and limbal mesenchymal stem cells (L-MSCs) may become a promising cell source for the development of these novel approaches, being established mainly within the rabbit model. In this study, we obtained and characterized rabbit L-MSCs and modified them with lentiviral transduction to express the green fluorescent protein EGFP (L-MSCs-EGFP). L-MSCs and L-MSCs-EGFP express not only stem cell markers specific for mesenchymal stem cells but also ABCG2, ABCB5, ALDH3A1, PAX6, and p63a specific for limbal epithelial stem cells (LESCs), as well as various cytokeratins (3/12, 15, 19). L-MSCs-EGFP have been proven to differentiate into adipogenic, osteogenic, and chondrogenic directions, as well as to transdifferentiate into epithelial cells. The possibility of using L-MSCs-EGFP to study the biocompatibility of various scaffolds developed to treat corneal pathologies was demonstrated. L-MSCs-EGFP may become a useful tool for studying regenerative processes occurring during the treatment of various corneal pathologies, including LSCD, with the use of cell-based technologies.

scholarly journals Isolation and Culture of Bovine Mammary Epithelial Stem Cells

2009 ◽  
Vol 71 (1) ◽  
pp. 15-19 ◽  
Author(s):  
Ji-Xia LI ◽  
Yong ZHANG ◽  
Li-Bing MA ◽  
Jian-Hong SUN ◽  
Bao-Ying YIN
Keyword(s):  
Stem Cells ◽  
Mammary Epithelial ◽  
Epithelial Stem Cells
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