Quantitation of human mammary epithelial stem cells with in vivo regenerative properties using a subrenal capsule xenotransplantation assay

2010 ◽  
Vol 5 (12) ◽  
pp. 1945-1956 ◽  
Author(s):  
Peter Eirew ◽  
John Stingl ◽  
Connie J Eaves
Keyword(s):  
Stem Cells ◽  
Mammary Epithelial ◽  
Epithelial Stem Cells ◽  
Human Mammary Epithelial ◽  
Subrenal Capsule

A method for quantifying normal human mammary epithelial stem cells with in vivo regenerative ability

2008 ◽  
Vol 14 (12) ◽  
pp. 1384-1389 ◽  
Author(s):  
Peter Eirew ◽  
John Stingl ◽  
Afshin Raouf ◽  
Gulisa Turashvili ◽  
Samuel Aparicio ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mammary Epithelial ◽  
Epithelial Stem Cells ◽  
Human Mammary Epithelial ◽  
Normal Human ◽  
Regenerative Ability

scholarly journals Dental Epithelial Stem Cells as a Source for Mammary Gland Regeneration and Milk Producing Cells In Vivo

Cells ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 1302 ◽  
Author(s):  
Jimenez-Rojo ◽  
Pagella ◽  
Harada ◽  
Mitsiadis
Keyword(s):  
Stem Cells ◽  
Mammary Gland ◽  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Genetic Lineage ◽  
Epithelial Stem Cells ◽  
Continuous Growth ◽  
Dental Tissues

The continuous growth of rodent incisors is ensured by clusters of mesenchymal and epithelial stem cells that are located at the posterior part of these teeth. Genetic lineage tracing studies have shown that dental epithelial stem cells (DESCs) are able to generate all epithelial cell populations within incisors during homeostasis. However, it remains unclear whether these cells have the ability to adopt alternative fates in response to extrinsic factors. Here, we have studied the plasticity of DESCs in the context of mammary gland regeneration. Transplantation of DESCs together with mammary epithelial cells into the mammary stroma resulted in the formation of chimeric ductal epithelial structures in which DESCs adopted all the possible mammary fates including milk-producing alveolar cells. In addition, when transplanted without mammary epithelial cells, DESCs developed branching rudiments and cysts. These in vivo findings demonstrate that when outside their niche, DESCs redirect their fates according to their new microenvironment and thus can contribute to the regeneration of non-dental tissues.

scholarly journals Intestinal epithelial cell-specific IGF1 promotes the expansion of intestinal stem cells during epithelial regeneration and functions on the intestinal immune homeostasis

2018 ◽  
Vol 315 (4) ◽  
pp. E638-E649 ◽  
Author(s):  
Yu Zheng ◽  
Yongli Song ◽  
Qi Han ◽  
Wenjie Liu ◽  
Jiuzhi Xu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Cell ◽  
Intestinal Epithelial Cell ◽  
High Throughput Sequencing ◽  
Secretory Cells ◽  
Intestinal Epithelial ◽  
Epithelial Stem Cells ◽  
Microbial Composition ◽  
Epithelial Regeneration

It is well known that insulin-like growth factor 1 (IGF1) acts as a trophic factor in small intestine under both physiological and pathophysiological conditions. However, it still lacks direct in vivo evidence of the functions of intestinal epithelial cell (IEC)-specific IGF1 under both normal and pathological conditions. Using IEC-specific IGF1-knockout (cKO) mice and Lgr5-eGFP-CreERT mice, we demonstrate that IEC-specific IGF1 can enhance nutrient uptake, reduce protein catabolism and energy consumption, and promote the proliferation and expansion of intestinal epithelial cells, including intestinal epithelial stem cells and intestinal secretory cells. Next, we showed that IEC-specific IGF1 renders IECs resistant to irradiation and promotes epithelial regeneration. Strikingly, transcriptome profiling assay revealed that many differentially expressed genes involved in the differentiation and maturation of lymphoid lineages were significantly suppressed in the cKO mice as compared with the control mice. We demonstrated that deletion of IGF1 in IECs enhances bacterial translocation to the mesenteric lymph nodes and liver. Furthermore, high-throughput sequencing of 16S ribosomal RNA genes of gut microbiota revealed that IEC-specific IGF1 loss profoundly affected the gut microbial composition at various levels of classification. Therefore, our findings shed light on the in vivo roles of IEC-specific IGF1 in intestinal homeostasis, epithelial regeneration, and immunity, broadening our current insights on IGF1 functions.

scholarly journals Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells.

1986 ◽  
Vol 103 (1) ◽  
pp. 49-62 ◽  
Author(s):  
A Schermer ◽  
S Galvin ◽  
T T Sun
Keyword(s):  
Stem Cells ◽  
Strong Support ◽  
Transitional Zone ◽  
Basal Cells ◽  
Epithelial Stem Cells ◽  
Corneal Epithelial ◽  
Differentiated Cells ◽  
Limbal Epithelium ◽  
Cell Layers

In this paper we present keratin expression data that lend strong support to a model of corneal epithelial maturation in which the stem cells are located in the limbus, the transitional zone between cornea and conjunctiva. Using a new monoclonal antibody, AE5, which is highly specific for a 64,000-mol-wt corneal keratin, designated RK3, we demonstrate that this keratin is localized in all cell layers of rabbit corneal epithelium, but only in the suprabasal layers of the limbal epithelium. Analysis of cultured corneal keratinocytes showed that they express sequentially three major keratin pairs. Early cultures consisting of a monolayer of "basal" cells express mainly the 50/58K keratins, exponentially growing cells synthesize additional 48/56K keratins, and postconfluent, heavily stratified cultures begin to express the 55/64K corneal keratins. Cell separation experiments showed that basal cells isolated from postconfluent cultures contain predominantly the 50/58K pair, whereas suprabasal cells contain additional 55/64K and 48/56K pairs. Basal cells of the older, postconfluent cultures, however, can become AE5 positive, indicating that suprabasal location is not a prerequisite for the expression of the 64K keratin. Taken together, these results suggest that the acidic 55K and basic 64K keratins represent markers for an advanced stage of corneal epithelial differentiation. The fact that epithelial basal cells of central cornea but not those of the limbus possess the 64K keratin therefore indicates that corneal basal cells are in a more differentiated state than limbal basal cells. These findings, coupled with the known centripetal migration of corneal epithelial cells, strongly suggest that corneal epithelial stem cells are located in the limbus, and that corneal basal cells correspond to "transient amplifying cells" in the scheme of "stem cells----transient amplifying cells----terminally differentiated cells."

scholarly journals Isolation and Culture of Bovine Mammary Epithelial Stem Cells

2009 ◽  
Vol 71 (1) ◽  
pp. 15-19 ◽  
Author(s):  
Ji-Xia LI ◽  
Yong ZHANG ◽  
Li-Bing MA ◽  
Jian-Hong SUN ◽  
Bao-Ying YIN
Keyword(s):  
Stem Cells ◽  
Mammary Epithelial ◽  
Epithelial Stem Cells
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