Molecular Mechanism of Action of Genistein and Related Phytoestrogens in Estrogen Receptor Dependent and Independent Growth of Breast Cancer Cells

2000 ◽  
Author(s):  
T. Thomas
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Cancer Cells ◽  
Molecular Mechanism ◽  
Mechanism Of Action ◽  
Breast Cancer Cells ◽  
Molecular Mechanism Of Action

scholarly journals Molecular Mechanism of Inhibitory Aryl Hydrocarbon Receptor—Estrogen Receptor/Sp1 Cross Talk in Breast Cancer Cells

2006 ◽  
Vol 20 (9) ◽  
pp. 2199-2214 ◽  
Author(s):  
Shaheen Khan ◽  
Rola Barhoumi ◽  
Robert Burghardt ◽  
Shengxi Liu ◽  
Kyounghyun Kim ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Aryl Hydrocarbon Receptor ◽  
Cancer Cells ◽  
Molecular Mechanism ◽  
Cross Talk ◽  
Breast Cancer Cells ◽  
Aryl Hydrocarbon

scholarly journals Estrogen receptor-α, RBCK1, and protein kinase C β 1 cooperate to regulate estrogen receptor-α gene expression

2012 ◽  
Vol 49 (3) ◽  
pp. 277-287 ◽  
Author(s):  
Nina Gustafsson Sheppard ◽  
Nina Heldring ◽  
Karin Dahlman-Wright
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Protein Kinase C ◽  
Protein Kinase ◽  
Cancer Cells ◽  
Molecular Mechanism ◽  
Breast Cancer Cells ◽  
Target Genes ◽  
Estrogen Receptor Α ◽  
Kinase C

Estrogen receptor α (ERα) is initially overexpressed in two-thirds of all breast cancers and is involved in its development and proliferation. We previously reported that the RanBP-type and C3HC4-type zinc finger containing 1 (RBCK1) interacts with the ERα promoter and that RBCK1 expression positively correlates with ERα levels, expression of ERα downstream target genes, and proliferation of breast cancer cells. Based on this, and that RBCK1 positively correlates with ERα expression in breast cancer samples, we propose RBCK1 as a potential therapeutic target in breast cancer acting as a modulator of ERα expression. To further explore this, the molecular mechanism by which RBCK1 regulates ERα expression has to be defined. Here, we show that ERα, RBCK1, and the RBCK1-interacting protein protein kinase C β 1 (PKCβI) co-occupy a previously identified ERα binding region in the proximal ERα promoter. We describe a number of mechanistic details of this complex including that RBCK1 recruitment to the ERα promoter B is facilitated by ERα, which in turn facilitates PKCβI recruitment and PKCβI-dependent histone modifications. Furthermore, ERα regulation of its own mRNA expression is facilitated by RBCK1 recruitment, suggesting an ERα coactivator function of RBCK1. The interaction between RBCK1 and ERα was dependent on the E3 ubiquitin ligase domain of RBCK1 and the activating function-1 domain of ERα. The ligand-binding function of ERα does not influence the interaction with RBCK1. In summary, our data provide insight into the molecular mechanism by which ERα expression is modulated in breast cancer cells.

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