A Competitive Displacement Assay to Detect Saxitoxin and Tetrodotoxin

1983 ◽  
Author(s):  
Stephen R. Davio ◽  
Paulito A. Fontelo
Keyword(s):  
Competitive Displacement ◽  
Displacement Assay

scholarly journals A colorimetric competitive displacement assay for the evaluation of catalytic peptides

2017 ◽  
Vol 15 (48) ◽  
pp. 10160-10163 ◽  
Author(s):  
Anneliese M. M. Gest ◽  
Erika M. Aguiluz ◽  
Malik T. Mays ◽  
Xinyu Liu ◽  
Eliza K. Neidhart ◽  
...  
Keyword(s):  
Competitive Displacement ◽  
Displacement Assay ◽  
Catalytic Peptides

An assay based on competitive dye displacement was adapted to detect the formation of aldol product in crude reaction mixtures, and was used to evaluate minimal peptide aldol catalysts.

A competitive displacement assay to detect saxitoxin and tetrodotoxin

1984 ◽  
Vol 141 (1) ◽  
pp. 199-204 ◽  
Author(s):  
Stephen R. Davio ◽  
Paulito A. Fontelo
Keyword(s):  
Competitive Displacement ◽  
Displacement Assay

scholarly journals Impairment of Methotrexate Transport Is Common in Osteosarcoma Tumor Samples

Sarcoma ◽  
2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Rebecca Sowers ◽  
Bethanne D. Wenzel ◽  
Condon Richardson ◽  
Paul A. Meyers ◽  
John H. Healey ◽  
...  
Keyword(s):  
High Frequency ◽  
High Dose ◽  
Reduced Folate Carrier ◽  
High Grade ◽  
Competitive Displacement ◽  
Conventional Dose ◽  
Dose Methotrexate ◽  
Displacement Assay ◽  
High Doses ◽  
High Grade Osteosarcoma

Osteosarcoma does not respond well to conventional dose methotrexate but does respond to high-dose methotrexate. Previous work has indicated that this resistance may be due to impaired transport of methotrexate across the cell membrane. In this study, the PT430 competitive displacement assay was adapted to evaluate methotrexate transport in 69 high-grade osteosarcoma tumor samples. All samples studied were shown to have relatively impaired methotrexate transport by PT430 assay. Ninety-nine percent of the samples had less than 20% PT430 displacement by methotrexate. Eighty-eight percent exhibited displacement by methotrexate at less than 50% of the displacement by trimetrexate. The high frequency of impaired transport suggests the presence of decreased functionality of the reduced folate carrier protein. The overwhelming presence of impaired transport may explain why methotrexate needs to be given in high doses to be effective in osteosarcoma therapy and suggests that reduced folate carrier-independent antifolates should be explored.

scholarly journals Cellular metabolic activity marker via selective turn-ON detection of serum albumin using an NBD-based fluoroprobe

2019 ◽  
Author(s):  
Tanoy Dutta ◽  
Kaushik Pal ◽  
Apurba Lal Koner
Keyword(s):  
Serum Albumin ◽  
Metabolic Activity ◽  
Fluorescence Enhancement ◽  
Site Specificity ◽  
Competitive Displacement ◽  
Site Specific ◽  
Turn On ◽  
Cellular Metabolic Activity ◽  
Displacement Assay ◽  
Activity Marker

AbstractA nitrobenzoxadiazole-based fluoroprobe (NBD-Bu) is designed to probe cellular metabolic activity in cancer and normal cells. NBD-Bu shows a significant fluorescence enhancement upon selective binding to serum albumin. The site specificity of NBD-Bu has been explored through a competitive displacement assay in the presence of site-specific markers such as warfarin and ibuprofen. Subsequently, high-resolution fluorescence microscopy results consolidated the potential of NBD-Bu for cellular imaging and detection of abnormal cellular metabolic activity.

scholarly journals Public service media in the age of SVoDs: A comparative study of PSM strategic responses in Flanders, Italy and the UK

2021 ◽  
pp. 016344372097290
Author(s):  
Alessandro D’Arma ◽  
Tim Raats ◽  
Jeanette Steemers
Keyword(s):  
New Media ◽  
Public Service ◽  
Government Support ◽  
Market Size ◽  
Online Video ◽  
Competitive Displacement ◽  
Strategic Responses ◽  
Streaming Services ◽  
The Uk ◽  
Different Levels

Netflix and other transnational online video streaming services are disrupting long-established arrangements in national television systems around the world. In this paper we analyse how public service media (PSM) organisations (key purveyors of societal goals in broadcasting) are responding to the fast-growing popularity of these new services. Drawing on Philip Napoli’s framework for analysing strategic responses by established media to threats of competitive displacement by new media, we find that the three PSM organisations in our study exhibit commonalities. Their responses have tended to follow a particular evolution starting with different levels of complacency and resistance before settling into more coherent strategies revolving around efforts to differentiate PSM offerings, while also diversifying into activities, primarily across new platforms, that mimic SVoD approaches and probe production collaborations. Beyond these similarities, however, we also find that a range of contextual factors (including path-dependency, the role and status of PSM in each country, the degree of additional government support, cultural factors and market size) help explain nuances in strategic responses between our three cases.

scholarly journals Estimating the cooperativity of PROTAC-induced ternary complexes using 19F NMR displacement assay

2021 ◽  
Author(s):  
Guilherme Castro ◽  
Alessio Ciulli
Keyword(s):  
Ternary Complexes ◽  
19F Nmr ◽  
Fluorine Nmr ◽  
Competition Binding ◽  
Displacement Assay

Cooperativity is an important parameter to understand the ternary complexes formed by protein degraders. We developed fluorine NMR competition binding experiments to determine cooperativity of PROTACs. We show applicability to...

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