Managing Sulphate Reducing Bacteria (SRB) Problem Associated With Produced Water In One Of The Oil Fields Of Oil India Limited (Oil) - A Case Study

2010 ◽  
Author(s):  
Mool Chand Nihalani ◽  
S. Verma ◽  
J. Kumar ◽  
H. Dubey ◽  
Nripendra Kumar Bharali ◽  
...  
Keyword(s):  
Produced Water ◽  
Oil Fields ◽  
Sulphate Reducing Bacteria ◽  
Reducing Bacteria

scholarly journals Sulphate-reducing bacterial community structure from produced water of the Periquito and Galo de Campina onshore oilfields in Brazil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Samyra Raquel Gonçalves Tiburcio ◽  
Andrew Macrae ◽  
Raquel Silva Peixoto ◽  
Caio Tavora Coelho da Costa Rachid ◽  
Felipe Raposo Passos Mansoldo ◽  
...  
Keyword(s):  
Bacterial Community Structure ◽  
Oil And Gas ◽  
Produced Water ◽  
Microbial Community Composition ◽  
Gas Production ◽  
Oil Fields ◽  
Accession Number ◽  
Oil And Gas Production ◽  
16S Rdna Pcr ◽  
Reducing Bacteria

AbstractSulphate-reducing bacteria (SRB) cause fouling, souring, corrosion and produce H2S during oil and gas production. Produced water obtained from Periquito (PQO) and Galo de Campina (GC) onshore oilfields in Brazil was investigated for SRB. Produced water with Postgate B, Postgate C and Baars media was incubated anaerobically for 20 days. DNA was extracted, 16S rDNA PCR amplified and fragments were sequenced using Illumina TruSeq. 4.2 million sequence reads were analysed and deposited at NCBI SAR accession number SRP149784. No significant differences in microbial community composition could be attributed to the different media but significant differences in the SRB were observed between the two oil fields. The dominant bacterial orders detected from both oilfields were Desulfovibrionales, Pseudomonadales and Enterobacteriales. The genus Pseudomonas was found predominantly in the GC oilfield and Pleomorphominas and Shewanella were features of the PQO oilfield. 11% and 7.6% of the sequences at GC and PQO were not classified at the genus level but could be partially identified at the order level. Relative abundances changed for Desulfovibrio from 29.8% at PQO to 16.1% at GC. Clostridium varied from 2.8% at PQO and 2.4% at GC. These data provide the first description of SRB from onshore produced water in Brazil and reinforce the importance of Desulfovibrionales, Pseudomonadales, and Enterobacteriales in produced water globally. Identifying potentially harmful microbes is an important first step in developing microbial solutions that prevent their proliferation.

Investigation into Mechanisms of Microbial Effects on Iron and Manganese Transformations in Artificially Recharged Groundwater

1988 ◽  
Vol 20 (3) ◽  
pp. 47-53 ◽  
Author(s):  
Yan Bao-rui
Keyword(s):  
Groundwater Quality ◽  
Biochemical Reaction ◽  
Reaction Products ◽  
Injection Wells ◽  
Iron Bacteria ◽  
Sulphate Reducing Bacteria ◽  
Prevention And Treatment ◽  
Reducing Bacteria ◽  
Iron And Manganese ◽  
Microbial Effects

After artificial recharging of groundwater some problems occurred, such as changes in groundwater quality, the silting up of recharge (injection) wells, etc. Therefore, the mechanisms of microbial effects on groundwater quality after artificial recharging were studied in Shanghai and the district of Changzhou. These problems were approached on the basis of the amounts of biochemical reaction products generated by the metabolism of iron bacteria, sulphate-reducing bacteria, Thiobacillusthioparus, and Thiobacillusdenitrificans. The experiments showed that in the transformations occurring and the siltation of recharge wells, microorganisms play an important role, due to the various chemical and biochemical activities. A water-rock-microorganisms system is proposed, and some methods for the prevention and treatment of these effects are given.

Corrosion of mild steel under diesel oil by sulphate-reducing bacteria

1984 ◽  
Vol 10 (1) ◽  
pp. 91-105 ◽  
Author(s):  
D.J. Crombie ◽  
G.J. Moody ◽  
J.D.R. Thomas
Keyword(s):  
Mild Steel ◽  
Diesel Oil ◽  
Sulphate Reducing Bacteria ◽  
Reducing Bacteria

Copper remediation by Eichhornia spp. and sulphate-reducing bacteria

2010 ◽  
Vol 173 (1-3) ◽  
pp. 231-235 ◽  
Author(s):  
Shailesh Dave ◽  
Maitry Damani ◽  
Devayani Tipre
Keyword(s):  
Sulphate Reducing Bacteria ◽  
Reducing Bacteria

The role of colonic sulphate-reducing bacteria in the pharmacology of heavy metals

1994 ◽  
Vol 3 (4) ◽  
pp. 357-360
Author(s):  
L Bolt ◽  
D C Ellwood ◽  
M J Hill ◽  
S Wootton ◽  
J H P Watson
Keyword(s):  
Heavy Metals ◽  
Sulphate Reducing Bacteria ◽  
Reducing Bacteria

Metagenomics Microbial Characterization of Production and Process Fluids in the Powder River Basin: Identification and Sources of Problematic Microorganisms Associated with SWD Facilities

2021 ◽  
Author(s):  
Michael Enzien ◽  
Sadie Starustka ◽  
Michael Gurecki ◽  
Trinity Fincher-Miller ◽  
Bryce Kuhn ◽  
...  
Keyword(s):  
Produced Water ◽  
Salt Water ◽  
Microbiologically Influenced Corrosion ◽  
Process Equipment ◽  
Drilling Fluids ◽  
Well Performance ◽  
Field Sample ◽  
Degrading Bacteria ◽  
Produced Waters ◽  
Reducing Bacteria

Abstract Inconsistent bacterial control and monitoring led to variability in Salt Water Disposal (SWD) well performance and injectivity creating excess costs in biocide applications and remedial work. A metagenomics study using Whole Genome Sequencing (WGS) was conducted to determine the source(s) of problematic microorganisms throughout the process life cycle: Freshwater> Drilling> Completion> Flowback> Produced water> SWD. A total of 30 metagenomes were collected from the 6 process stages and identification and quantification of the major microbial taxa from each of these stages were identified. "Taxonomy to Function" associations were identified for all the major taxa found in the SWD fluids. WGS was performed on positive Sulfate Reducing Bacteria (SRB) and Acid Producing Bacteria (APB) media bottles inoculated in the field for a Flowback sample. Four of the six major taxa found in SWD samples are considered groups of microorganisms known to cause microbiologically influenced corrosion (MIC): Clostridia, methanogens, SRB and Iron Reducing bacteria. Thermovirga and Thermotagae, were the two most abundant taxa found in SWD samples, both thermophilic halophilic fermenting bacteria. The Fe reducing bacteria Shewanella was only detected in Drilling and SWD fluids suggesting its source was Drilling fluids. Completion fluid metagenome profiles from two separate sites followed similar patterns. During middle of completions Proteobacteria phyla were dominant taxa represented mostly by Pseudomonas. Other abundant phyla were all characteristic of polymer degrading bacteria. None of these taxa were dominant populations identified in SWD waters. Fresh water only shared similar taxa with Drilling and Completion fluids. A few minor taxa from Drilling and Completion stages show up as significant taxa in SWD fluids. The majority of taxa found in SWD samples appear to originate from Flowback and Produced waters, although at lower abundances than found in SWD samples. It cannot be determined if the microorganisms found in Flowback and Produced waters were endemic to the formation or come from contaminated source waters, i.e. process equipment used to store and transport water sources. Petrotoga mobilis was the dominant population of bacteria that grew in both media bottles, 96% and 77% for SRB and APB, respectively, while Petrotoga was detected at 14% in the field sample. The most abundant bacteria detected in field sample were Clostridia (38%) while only 2.7% were detected in APB media. SRB media bottle had 0.18% SRB detected by WGS; APB media had 9% SRB population abundance. No SRB were detected in corresponding field sample or below detectable limits (BDL) for WGS methods (<0.01%). WGS was forensically used to successfully identify type and source of problematic microorganism in SWD facilities. Results from media bottle and field sample comparisons stress the importance of developing improved field monitoring techniques that more accurately detect the dominant microorganisms.

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